Single-Cell DNA Sequencing Reveals Punctuated and Gradual Clonal Evolution in Hepatocellular Carcinoma

Guo, Lin; Yi, Xianfu; Chen, Lu; Zhang, Ti; Guo, Hua; Chen, Ziye; Cheng, Jinghui; Cao, Qi; Liu, Hengkang; Hou, Chunyu; Qi, Lisha; Zhu, Zhiyan; Liu, Yucun; Kong, Ruirui; Zhang, Chong; Zhou, Xiao‐Hua; Zhang, Zemin; Song, Tianqiang; Xue, Ruyi; Zhang, Ning

doi:10.1053/j.gastro.2021.08.052

Cited by 39 publications

(23 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, SVEP1 has been proven to play an important role in preventing tumor cells from invading bone niches as a key cell adhesion molecule ( Glait-Santar et al, 2012 ). In our previous studies, we found and reported that SVEP1, as a promising novel diagnostic and prognostic biomarker, plays various regulatory roles in the malignant progression of hepatocellular carcinoma ( Chen et al, 2020 ; Gong et al, 2022a ; Guo et al, 2022 ). In this study, we verified that the expression level of SVEP1 was dramatically decreased in the ICC tumor tissues when compared with the corresponding adjacent tissues and that it was negatively correlated with OS and DFS by using IHC in a 47 ICC patient tissue microarray.…”

Section: Discussionmentioning

confidence: 99%

“…SVEP1 is a newly reported secreted multidomain ECM, which is involved in embryonic development, cell adhesion and differentiation, and maintaining tissue microenvironment homeostasis Morooka et al, 2017;Samuelov et al, 2017). More importantly, in our previous studies, we found and reported that SVEP1 also plays a critical role in tumor heterogeneity (Guo et al, 2022), proliferation and metastasis (Chen et al, 2020), and tumor stem cell-like phenotype maintenance (Gong et al, 2022a). In this study, we firstly present the expression profile of SVEP1 and identified abnormal neovascularization in ICC.…”

Section: Introductionmentioning

confidence: 92%

See 1 more Smart Citation

The impact of decreased expression of SVEP1 on abnormal neovascularization and poor prognosis in patients with intrahepatic cholangiocarcinoma

Chen

He²,

Han³

et al. 2023

Front. Genet.

Self Cite

View full text Add to dashboard Cite

Introduction: Intrahepatic cholangiocarcinoma (ICC) is one of the most highly heterogeneous malignant solid tumors; it is generally insensitive to clinical treatment and has a poor prognosis. Evidence suggests that abnormal neovascularization in the tumor microenvironment is an important cause of treatment resistance as well as recurrence and metastasis, but the key regulatory molecules are still largely unknown and should be identified.Method: We assessed the novel extracellular matrix protein (ECM) Sushi, von Willebrand factor type A, EGF and pentraxin containing 1 (SVEP1) expression pattern in the ICC by using immunohistochemistry. Multiplex immunofluorescence and Kaplan-Meier analysis were applied to explore the correlation between the low expression of SVEP1 and abnormal blood vessels and the clinical prognosis of ICC.Results: Our study showed that the expression of SVEP1 in most ICC samples was relatively lower than in the adjacent tissues. Statistical analysis suggested that patients with decreased SVEP1 expression always had shorter overall survival (OS) and disease-free survival (DFS). Moreover, the expression of SVEP1 was negatively correlated with the proportion of abnormal neovascularization in the tumor microenvironment of the ICC. Consistently, the key molecule of promoting vascular normalization, Ang-1, is positively correlated with the SVEP1 expression and prognosis in the ICC. In addition, the proportion of high Ki-67 expression was higher in the ICC samples with low SVEP1 expression, suggesting that the SVEP1 low expressed sample is in a malignant phenotype with high proliferation.Conclusion: This study reveals that SVEP1 is a promising prognostic biomarker for ICC and provides fresh insight into the role and potential new mechanism of abnormal neovascularization in ICC progression.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

The impact of decreased expression of SVEP1 on abnormal neovascularization and poor prognosis in patients with intrahepatic cholangiocarcinoma

Chen

He²,

Han³

et al. 2023

Front. Genet.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Guo et al. performed scDNA-seq and scRNA-seq on 28,975 cells from 14 patients with HCC, proposing a novel dual-phase copy number evolution (DPCNE) model ( 125 ). Accumulation of Copy number alterations (CNAs), elicited by genome instability, plays a pivotal role in hepatocarcinogenesis.…”

Section: Applications Of Single-cell Sequencing In Liver Cancermentioning

confidence: 99%

Single-Cell Sequencing and Its Applications in Liver Cancer

Tian

2022

Front. Oncol.

View full text Add to dashboard Cite

As one of the most lethal cancers, primary liver cancer (PLC) has high tumor heterogeneity, including the heterogeneity between cancer cells. Traditional methods which have been used to identify tumor heterogeneity for a long time are based on large mixed cell samples, and the research results usually show average level of the cell population, ignoring the heterogeneity between cancer cells. In recent years, single-cell sequencing has been increasingly applied to the studies of PLCs. It can detect the heterogeneity between cancer cells, distinguish each cell subgroup in the tumor microenvironment (TME), and also reveal the clonal characteristics of cancer cells, contributing to understand the evolution of tumor. Here, we introduce the process of single-cell sequencing, review the applications of single-cell sequencing in the heterogeneity of cancer cells, TMEs, oncogenesis, and metastatic mechanisms of liver cancer, and discuss some of the current challenges in the field.

show abstract

“…The spatial heterogeneity of tumors is reflected in that tumor cells at the tumor margin exhibit high invasive and metastatic characteristics, while cells within the tumor tissue maximize self-proliferation by promoting angiogenesis and increasing metabolism. Multi-omics fusion studies have found that the genome, transcriptome, proteome, and metabolome of HCC take the partial area in the microenvironment as the unit, and there is great heterogeneity in different areas ( Guo et al, 2022 ).…”

Section: Heterogeneity Of Liver Cancer Tumor Cellsmentioning

confidence: 99%

Advances in single-cell sequencing technology in the field of hepatocellular carcinoma

Qin

Zhao²,

He³

et al. 2022

Front. Genet.

View full text Add to dashboard Cite

Tumors are a class of diseases characterized by altered genetic information and uncontrolled growth. Sequencing technology provide researchers with a better way to explore specific tumor pathogenesis. In recent years, single-cell sequencing technology has shone in tumor research, especially in the study of liver cancer, revealing phenomena that were unexplored by previous studies. Single-cell sequencing (SCS) is a technique for sequencing the cellular genome, transcriptome, epigenome, proteomics, or metabolomics after dissociation of tissues into single cells. Compared with traditional bulk sequencing, single-cell sequencing can dissect human tumors at single-cell resolution, finely delineate different cell types, and reveal the heterogeneity of tumor cells. In view of the diverse pathological types and complex pathogenesis of hepatocellular carcinoma (HCC), the study of the heterogeneity among tumor cells can help improve its clinical diagnosis, treatment and prognostic judgment. On this basis, SCS has revolutionized our understanding of tumor heterogeneity, tumor immune microenvironment, and clonal evolution of tumor cells. This review summarizes the basic process and development of single-cell sequencing technology and its increasing role in the field of hepatocellular carcinoma.

show abstract

Single-Cell DNA Sequencing Reveals Punctuated and Gradual Clonal Evolution in Hepatocellular Carcinoma

Cited by 39 publications

References 51 publications

The impact of decreased expression of SVEP1 on abnormal neovascularization and poor prognosis in patients with intrahepatic cholangiocarcinoma

The impact of decreased expression of SVEP1 on abnormal neovascularization and poor prognosis in patients with intrahepatic cholangiocarcinoma

Single-Cell Sequencing and Its Applications in Liver Cancer

Advances in single-cell sequencing technology in the field of hepatocellular carcinoma

Contact Info

Product

Resources

About