Single‐cell atlas reveals a distinct immune profile fostered by T cell‐B cell crosstalk in triple negative breast cancer

Abstract

Background: Characterizing the unique immune microenvironment of each tumor is of great importance for better predicting prognosis and guiding cancer immunotherapy. However, the unique features of the immune microenvironment of triple negative breast cancer (TNBC) compared with other subtypes of breast cancer remain elusive. Therefore, we aimed to depict and compare the immune landscape among TNBC, human epidermal growth factor receptor 2-positive (HER2 + ) breast cancer, and luminal-like breast cancer.Methods: Single-cell RNA sequencing (scRNA-seq) was performed on CD45 + immune cells isolated from human normal breast tissues and primary breast tumors of various subtypes. By analyzing the scRNA-seq data, immune cell clusters were identified and their proportions as well as transcriptome features were compared among TNBC, human HER2 + breast cancer, and luminal-like breast

Identification of cuproptosis-related miRNAs in triple-negative breast cancer and analysis of the miRNA–mRNA regulatory network

“…VCAM-1 in endothelial cells stimulates TNBC cells to adhere to endothelial cells and mediates the migration of TNBC cells through the endothelial cell layer (mediated by CTGF) [ 49 ]. Regulatory T cells (Treg) are enriched in TNBC tissues, CD8 + T cells are depleted, and some cytotoxic CD8 + T cells are in a state of transition to failure, indicating that their tumor-toxic activity is inhibited [ 50 ]. These cells may be targeted for the treatment of TNBC.…”

Cholesterol efflux from C1QB-expressing macrophages is associated with resistance to chimeric antigen receptor T cell therapy in primary refractory diffuse large B cell lymphoma

“…We then identified all the significant interactions (ligand-receptor pairs) for a list of T cell exhaustion-related pathways and visualized their average expression among different cell subtypes by ComplexHeatmap (version 2.6.2) 75 R package. For a given pair of ligand and receptor, we used a violin plot to visualize their expression levels across groups 76 .…”

Dendritic cells: the yin and yang in disease progression

“…Of all existing types of DCs, pDCs and moDCs seem to have contradictory roles in cancer immunity. Evidence has demonstrated that pDCs infiltrate different types of tumors and are associated with poor outcomes ( 54 ), due to the expression of inhibitory markers including lymphocyte-activation gene (LAG)-3, PD-1, and CTLA-4 ( 55 – 57 ), the release of immunosuppressive cytokines (e.g., IL-10, TGF-β, and prostaglandin E2) ( 58 ) as well as the expansion and Treg accumulation ( 59 ). However, it has been suggested that pDCs may have a lytic ability against tumor cells ( 60 , 61 ).…”