Single-Cell Analysis Using Drop-on-Demand Inkjet Printing and Probe Electrospray Ionization Mass Spectrometry

Chen, Fengming; Lin, Lei; Zhang, Jie; He, Ziyi; Uchiyama, Katsumi; Lin, Jin‐Ming

doi:10.1021/acs.analchem.5b04749

Cited by 132 publications

(101 citation statements)

References 49 publications

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“…For as-mentioned techniques, analytes are required to be activated with corresponding responses for the analysis of complex mixture [104], MS approach is a more powerful tool, since a wide range of molecules could be label-free, specifically and sensitively detected by it with providing information on the molecular weights and chemical structures of the analytes [105][106][107][108]. Recently, ambient ionization technique has been introduced into MS and draw great attentions.…”

Section: Ms Methodsmentioning

confidence: 99%

Chemical and biochemical analysis on lab-on-a-chip devices fabricated using three-dimensional printing

Zhang

2016

TrAC Trends in Analytical Chemistry

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Section: Ms Methodsmentioning

confidence: 99%

Chemical and biochemical analysis on lab-on-a-chip devices fabricated using three-dimensional printing

Zhang

2016

TrAC Trends in Analytical Chemistry

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“…By spraying the solvent vapor to the needle tip, metabolites were electrosprayed from the tip of the probe. Recently, Chen et al developed a method for single-cell analysis and lipid pro ling by combining drop-on-demand inkjet cell printing and PESI-MS. 89) rough inkjet sampling of a cell suspension, droplets including single cells with a volume of ∼500 pL were generated. ey were precisely dripped onto a tungsten-made electrospray ionization needle, and immediately spayed under a high-voltage electric eld.…”

Section: Probe Electrospray Ionizationmentioning

confidence: 99%

Desorption in Mass Spectrometry

et al. 2017

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In mass spectrometry, analytes must be released in the gas phase. ere are two representative methods for the gasi cation of the condensed samples, i.e., ablation and desorption. While ablation is based on the explosion induced by the energy accumulated in the condensed matrix, desorption is a single molecular process taking place on the surface. In this paper, desorption methods for mass spectrometry developed in our laboratory: ash heating/rapid cooling, Leidenfrost phenomenon-assisted thermal desorption (LPTD), solid/solid friction, liquid/solid friction, electrospray droplet impact (EDI) ionization/desorption, and probe electrospray ionization (PESI), will be described. All the methods are concerned with the surface and interface phenomena. e concept of how to desorb less-volatility compounds from the surface will be discussed.Copyright © 2017 Dilshadbek Tursunbayevich Usmanov, Satoshi Ninomiya, Lee Chuin Chen, Subhrakanti Saha, Mridul Kanti Mandal, Yuji Sakai, Rio Takaishi, Ahsan Habib, Kenzo Hiraoka, Kentaro Yoshimura, Sen Takeda, Hiroshi Wada, and Hiroshi Nonami. is is an open access article distributed under the terms of Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.Please cite this article as: Mass Spectrom (Tokyo) 2017; 6(2): S0059 Keywords: desorption, ablation, thermal desorption, Leidenfrost phenomenon, ash heating, electrospray droplet impact ionization, probe electrospray ionization (Received September 20, 2016; Accepted January 4, 2017) RATIONALE: ABLATION VS. DESORPTION e two technical terms, ablation and desorption, should be distinguished rigorously. As shown in Fig. 1, ablation is induced by explosion of the matrix or substrate whereas desorption is a single molecular event, i.e., detachment of a single molecule from the surface. e antonym of desorption is adsorption.In matrix assisted laser desorption/ionization (MALDI), for example, the photon energy is ultimately converted to thermal energy and ablation of the matrix takes place above the critical laser power (Fig. 1(a)). In ablation, the ablated materials are mainly composed of aggregates. us the ions observed in MALDI are only a small part of materials ablated. In this respect, MALDI is more likely matrixassisted laser "ablation" ionization. In secondary ion mass spectrometry (SIMS), primary projectiles such as Ar, and (Ar) n + are used. In SIMS using atomic ion projectiles, the sputtering is caused by the cascade collisions in the sample. us the useful yield de ned as [total gas phase ions detected] divided by [total amount of sample sputtered] is rather low. e small useful yields for MALDI and SIMS (10 −5 -10) originate from the fact that photons or primary beams penetrate into the target materials leading to the sputtering of the substrate. In contrast, the useful yield for desorption-based techniques could be much higher than MALDI and SIMS if the desorbed molecules are ionized by high-e cien...

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“…To better understand and make use of this ionization source, significant efforts have been made over the past decades and have resulted in the developments of a multitude of theories on the mechanism of ionization process, for both direct current (DC) and alternating current (AC) ESI. Based on these theories, a variety of techniques and new ionization sources have also been developed to meet different analytical needs …”

Section: Introductionmentioning

confidence: 99%

Electric modeling and characterization of pulsed high‐voltage nanoelectrospray ionization sources by a miniature ion trap mass spectrometer

Wu²,

Tang

et al. 2019

J Mass Spectrom

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A better understanding of nanoelectrospray ionization (nano‐ESI) would be beneficial in further improving the performances of nano‐ESI. In this work, the pulsed high‐voltage (HV) nano‐ESI has been electrically modeled and then systematically characterized by both voltage‐current and mass spectrometry measurements. First, the equivalent resistance of a nano‐ESI source changes with respect to both emitter tip diameter and the HV applied. Increased voltage could improve both spray current and ionization efficiency of the pulsed HV nano‐ESI. Compared with conventional DC HV method, a pulsed HV has less heating effect on the capillary tip and thus allowing the application of a much higher voltage onto a nano‐ESI source. As a result, a pulsed HV nano‐ESI could further boost the ionization efficiency of nano‐ESI by employing even higher voltages than conventional DC nano‐ESI sources.

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Single-Cell Analysis Using Drop-on-Demand Inkjet Printing and Probe Electrospray Ionization Mass Spectrometry

Cited by 132 publications

References 49 publications

Chemical and biochemical analysis on lab-on-a-chip devices fabricated using three-dimensional printing

Chemical and biochemical analysis on lab-on-a-chip devices fabricated using three-dimensional printing

Desorption in Mass Spectrometry

Electric modeling and characterization of pulsed high‐voltage nanoelectrospray ionization sources by a miniature ion trap mass spectrometer

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