Single cell analysis reveals unexpected growth phenotype of <i>S. cerevisiae</i>

Kortmann, Hendrik; Blank, Lars M.; Schmid, Andreas

doi:10.1002/cyto.a.20684

Cited by 26 publications

(31 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5a, b). For single cell separation, e.g., into a micro-titer plate well (Kortmann et al 2009b) the single cell recovery could depend on cell size. While cells with diameters above 10 lm cannot enter remaining dead volume, bacterial cells with diameters as small as 1 lm have to be recovered with great care.…”

Section: Applicability and Leakage Pressure Of The On-tube-sealmentioning

confidence: 99%

Pressure-resistant and reversible on-tube-sealing for microfluidics

Fritzsch

Kortmann

Lonzynski

et al. 2010

Microfluid Nanofluid

Self Cite

View full text Add to dashboard Cite

We present the design, manufacturing, and characterization of a novel miniaturized on-tube-seal configuration for microfluidic devices. The seal is based on a previously developed world-to-chip spring-based interface by Kortmann et al. (Lab Chip 9:1455-1460, 2009a, which enables rapid and reliable microfluidic connections. In this study, the dead volumes, the contact pressure, the discontinuous fluidic-profile transmission, and the space requirements were significantly optimized by a new on-tube-seal configuration. Maintaining the advantages of the previously described interface, the new on-tube-seal configuration has a dead volume of only 18 nl, withstands pressures higher than 2,800 kPa with only 3.8 N applied contact force, and allows continuous capillary to chip fluidic profile transmission. The on-tube-seal configuration consists of a miniaturized o-ring (0.5 9 0.3 mm) integrated into a 1/16 00 tubing that reduces space requirements to a minimal sealing grid of 1.59 mm and is easily adaptable to any planar channel opening of micro fluidic devices. In summary, we present a novel combination of gasket and tubing, which we termed on-tube-seal that allows simple, rapid, and reliable world-to-chip sealing.

show abstract

Section: Applicability and Leakage Pressure Of The On-tube-sealmentioning

confidence: 99%

Pressure-resistant and reversible on-tube-sealing for microfluidics

Fritzsch

Kortmann

Lonzynski

et al. 2010

Microfluid Nanofluid

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is also useful for single cell study [3]. However, conventional DEP manipulation requires delicate photolithography and thin-film processes to fabricate metal electrodes.…”

Section: Introductionmentioning

confidence: 99%

Optically-induced dielectrophoresis using polymer materials for biomedical applications

Lee

Lin

et al. 2009

TRANSDUCERS 2009 - 2009 International Solid-State Sensors, Actuators and Microsystems Conference

View full text Add to dashboard Cite

This paper briefly demonstrates three kinds of microfluidic modules by using a polymer-based optically-induced dielectrophoresis (ODEP) platform. Polymer materials with excellent optoelectronic efficiency can be mass-produced at room temperature using a spin-coating process. This approach can easily facilitate the mass-fabrication process of the ODEP chip. The first function presented in this platform is a cell lysis device. Experimental result shows that it can selectively lyse a single cell within a group of cells, which is a function cannot be performed using traditional tools. Besides, the function that selectively disrupts the cell membrane without damaging the nucleus can also be achieved. Second, an optically-induced flow cytometry is reported, which enables one to continuously count and to sort microparticles based on the ODEP forces. No complicated fabrication process is needed to achieve the cytometry function. The particles can be focused by the ODEP forces and detect through a pair of optical fibers embedded into fiber channels in real time. Third, a new method which can quickly separate micro-particles with different sizes using this platform is demonstrated. Three operation modes including light wavelength, light intensity and light width are successfully used to separate the microparticles with a high-throughput. The developed polymer-based ODEP platform can be widely applied in biomedical research.

show abstract

“…Growth carried on in a microtiter plate can be monitored at macroscopic scale using optical density (OD) measurements. A preliminary sorting-step is required to inoculate each well with a single cell, involving the use of speciWc equipment such as a sorting cytometer [8] or a labon-chip microXuidic system [9]. An alternative solution is to use serial dilutions of the cell culture sample until a high probability of inoculating each well with a single cell is reached [10].…”

Section: Introductionmentioning

confidence: 99%

Single-cell analysis of S. cerevisiae growth recovery after a sublethal heat-stress applied during an alcoholic fermentation

Tibayrenc

Preziosi-Belloy

Ghommidh

2010

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

Interest in bioethanol production has experienced a resurgence in the last few years. Poor temperature control in industrial fermentation tanks exposes the yeast cells used for this production to intermittent heat stress which impairs fermentation efficiency. Therefore, there is a need for yeast strains with improved tolerance, able to recover from such temperature variations. Accordingly, this paper reports the development of methods for the characterization of Saccharomyces cerevisiae growth recovery after a sublethal heat stress. Single-cell measurements were carried out in order to detect cell-to-cell variability. Alcoholic batch fermentations were performed on a defined medium in a 2 l instrumented bioreactor. A rapid temperature shift from 33 to 43 °C was applied when ethanol concentration reached 50 g l⁻¹. Samples were collected at different times after the temperature shift. Single cell growth capability, lag-time and initial growth rate were determined by monitoring the growth of a statistically significant number of cells after agar medium plating. The rapid temperature shift resulted in an immediate arrest of growth and triggered a progressive loss of cultivability from 100 to 0.0001% within 8 h. Heat-injured cells were able to recover their growth capability on agar medium after a lag phase. Lag-time was longer and more widely distributed as the time of heat exposure increased. Thus, lag-time distribution gives an insight into strain sensitivity to heat-stress, and could be helpful for the selection of yeast strains of technological interest.

show abstract

Single cell analysis reveals unexpected growth phenotype of S. cerevisiae

Cited by 26 publications

References 62 publications

Pressure-resistant and reversible on-tube-sealing for microfluidics

Pressure-resistant and reversible on-tube-sealing for microfluidics

Optically-induced dielectrophoresis using polymer materials for biomedical applications

Single-cell analysis of S. cerevisiae growth recovery after a sublethal heat-stress applied during an alcoholic fermentation

Contact Info

Product

Resources

About