Single-cell analysis of the methanogenic archaeon Methanosarcina soligelidi from Siberian permafrost by means of confocal Raman microspectrocopy for astrobiological research

Serrano, Paloma; Wagner, Dirk; Böttger, Ute; Vera, Jean‐Pierre de; Hermelink, Antje

doi:10.1016/j.pss.2013.10.002

Cited by 17 publications

(14 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…e RAMAN and FTIR) spectroscopy is a non-destructive and non-invasive method which provides information about the molecular composition and structure within a sample. It has been widely used in astrobiology to characterize either minerals or organic matter 6 , 35 – 37 and the Raman Laser Spectrometer (RLS) is an important part of the payload of the 2018 ExoMars rover 38 . To investigate whether vibrational signals from Yersinia remained detectable after mineralization, we analyzed non-mineralized (control) and mineralized cells using RAMAN and FTIR spectroscopy (Figs 2 and 3 ).…”

Section: Resultsmentioning

confidence: 99%

Mineralization and Preservation of an extremotolerant Bacterium Isolated from an Early Mars Analog Environment

Gaboyer

Milbeau

Bohmeier

et al. 2017

Sci Rep

View full text Add to dashboard Cite

The artificial mineralization of a polyresistant bacterial strain isolated from an acidic, oligotrophic lake was carried out to better understand microbial (i) early mineralization and (ii) potential for further fossilisation. Mineralization was conducted in mineral matrixes commonly found on Mars and Early-Earth, silica and gypsum, for 6 months. Samples were analyzed using microbiological (survival rates), morphological (electron microscopy), biochemical (GC-MS, Microarray immunoassay, Rock-Eval) and spectroscopic (EDX, FTIR, RAMAN spectroscopy) methods. We also investigated the impact of physiological status on mineralization and long-term fossilisation by exposing cells or not to Mars-related stresses (desiccation and radiation). Bacterial populations remained viable after 6 months although the kinetics of mineralization and cell-mineral interactions depended on the nature of minerals. Detection of biosignatures strongly depended on analytical methods, successful with FTIR and EDX but not with RAMAN and immunoassays. Neither influence of stress exposure, nor qualitative and quantitative changes of detected molecules were observed as a function of mineralization time and matrix. Rock-Eval analysis suggests that potential for preservation on geological times may be possible only with moderate diagenetic and metamorphic conditions. The implications of our results for microfossil preservation in the geological record of Earth as well as on Mars are discussed.

show abstract

Section: Resultsmentioning

confidence: 99%

Mineralization and Preservation of an extremotolerant Bacterium Isolated from an Early Mars Analog Environment

Gaboyer

Milbeau

Bohmeier

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Two different approaches of analysis were undertaken: (i) overall metabolic phenotype changes using Rametrix TM chemometric methods and (ii) correlation of specific Raman bands with their corresponding biomolecules and comparisons with data obtained by well-established analytical methods (i.e., UPLC and GC-FID). While more advanced instrumentation and single-cell methodologies exist (Wu et al, 2011;Serrano et al, 2014), the purpose of this research was to develop phenotyping capabilities with a relatively inexpensive and portable Raman microscope (with 10× objective imaging) that could have broad appeal to microbiology labs. Several Raman devices are now capable of field research, and Rametrix TM technologies may be able to be implemented there as well.…”

Section: Discussionmentioning

confidence: 99%

“…The application of Raman spectroscopy is an expanding field and it includes fermentation monitoring (Sivakesava, Irudayaraj & Demirci, 2001;Ewanick et al, 2013;Olson et al, 2016;Zu et al, 2016), detection and identification of microorganisms (Nelson, Manoharan & Sperry, 1992;Kirschner et al, 2001;Pahlow et al, 2015), monitoring the kinetics of germination of individual Clostridium difficile spores (Wang et al, 2015) and detection of its toxins (Koya et al, 2018). It has been also demonstrated that Raman spectroscopy can be used in near-real time phenotyping of Escherichia coli exposed to alcohol (Zu et al, 2014;Zu, Athamneh & Senger, 2016) and antibiotics (Athamneh et al, 2014), single cell phenotyping (Wu et al, 2011;Serrano et al, 2014;Sun et al, 2015;García-Timermans et al, 2019), and characterizing phenotypic differences among E. coli enriched for 1-butanol tolerance (Freedman et al, 2016;Zu et al, 2014). Thus, Raman spectroscopy was selected as an effective method for probing changes in Synechocystis phenotypes in near real-time when exposed to external stimuli.…”

Section: Introductionmentioning

confidence: 99%

Characterizing metabolic stress-induced phenotypes ofSynechocystisPCC6803 with Raman spectroscopy

Tanniche

Collakova

Denbow

et al. 2020

PeerJ

View full text Add to dashboard Cite

Background During their long evolution, Synechocystis sp. PCC6803 developed a remarkable capacity to acclimate to diverse environmental conditions. In this study, Raman spectroscopy and Raman chemometrics tools (RametrixTM) were employed to investigate the phenotypic changes in response to external stressors and correlate specific Raman bands with their corresponding biomolecules determined with widely used analytical methods. Methods Synechocystis cells were grown in the presence of (i) acetate (7.5–30 mM), (ii) NaCl (50–150 mM) and (iii) limiting levels of MgSO4 (0–62.5 mM) in BG-11 media. Principal component analysis (PCA) and discriminant analysis of PCs (DAPC) were performed with the RametrixTM LITE Toolbox for MATLABⓇ. Next, validation of these models was realized via RametrixTM PRO Toolbox where prediction of accuracy, sensitivity, and specificity for an unknown Raman spectrum was calculated. These analyses were coupled with statistical tests (ANOVA and pairwise comparison) to determine statistically significant changes in the phenotypic responses. Finally, amino acid and fatty acid levels were measured with well-established analytical methods. The obtained data were correlated with previously established Raman bands assigned to these biomolecules. Results Distinguishable clusters representative of phenotypic responses were observed based on the external stimuli (i.e., acetate, NaCl, MgSO4, and controls grown on BG-11 medium) or its concentration when analyzing separately. For all these cases, RametrixTM PRO was able to predict efficiently the corresponding concentration in the culture media for an unknown Raman spectra with accuracy, sensitivity and specificity exceeding random chance. Finally, correlations (R > 0.7) were observed for all amino acids and fatty acids between well-established analytical methods and Raman bands.

show abstract

“…The rover will have a Raman spectrometer on board. Current studies describe how this instrument may be used to characterize potential methanogenic archaea, based on experiments with M. soligelidi on two different Martian-like soils [ 70 , 71 ].…”

Section: Simulating Extraterrestrial Conditionsmentioning

confidence: 99%

“…Methanogens would perfectly fit into this subsurface environment especially due to their ecophysiological capabilities to tolerate and endure extreme conditions. Several studies focus on the potential of methanogens to grow under Martian-like environmental conditions [ 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 ]. The detection of subsurface water aquifers on the icy moons in the outer Solar System extended the group of potential habitats by far.…”

Section: Introductionmentioning

confidence: 99%

Assessing the Ecophysiology of Methanogens in the Context of Recent Astrobiological and Planetological Studies

Taubner

Schleper

Firneis

et al. 2015

Life

View full text Add to dashboard Cite

Among all known microbes capable of thriving under extreme and, therefore, potentially extraterrestrial environmental conditions, methanogens from the domain Archaea are intriguing organisms. This is due to their broad metabolic versatility, enormous diversity, and ability to grow under extreme environmental conditions. Several studies revealed that growth conditions of methanogens are compatible with environmental conditions on extraterrestrial bodies throughout the Solar System. Hence, life in the Solar System might not be limited to the classical habitable zone. In this contribution we assess the main ecophysiological characteristics of methanogens and compare these to the environmental conditions of putative habitats in the Solar System, in particular Mars and icy moons. Eventually, we give an outlook on the feasibility and the necessity of future astrobiological studies concerning methanogens.

show abstract

Single-cell analysis of the methanogenic archaeon Methanosarcina soligelidi from Siberian permafrost by means of confocal Raman microspectrocopy for astrobiological research

Cited by 17 publications

References 33 publications

Mineralization and Preservation of an extremotolerant Bacterium Isolated from an Early Mars Analog Environment

Mineralization and Preservation of an extremotolerant Bacterium Isolated from an Early Mars Analog Environment

Characterizing metabolic stress-induced phenotypes ofSynechocystisPCC6803 with Raman spectroscopy

Assessing the Ecophysiology of Methanogens in the Context of Recent Astrobiological and Planetological Studies

Contact Info

Product

Resources

About