Single cell analysis of host response to helminth infection reveals the clonal breadth, heterogeneity, and tissue-specific programming of the responding CD4+ T cell repertoire

Brown, IK; Dyjack, N; Miller, M. M.; Krovi, H; Rios, C; Woolaver, R; Harmacek, L; Tu, T; O'Conner, BP; Danhorn, T; Vestal, B; Gapin, L; Pinilla, C; Seibold, M.A.; Scott-Browne, J; Santos, RG; Reinhardt, RL

doi:10.21417/ikb2021pp

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“…When using this approach, determination of the T cell receptor sequences of interest is a vital component in the process. T cell sequence clustering approaches can be used with bulk or expanded TCR sequencing data in furtherance of this goal (Brown et al., 2021; Falta et al., 2021; Greaves et al., 2021)…”

Section: Commentarymentioning

confidence: 99%

Identification of B Cell and T Cell Epitopes Using Synthetic Peptide Combinatorial Libraries

et al. 2022

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This unit presents a combinatorial library method that consists of the synthesis and screening of mixture-based synthetic combinatorial libraries of peptide molecules. The protocols employ peptide libraries to identify peptides recognized by mAbs and T cells. The first protocol uses a positional scanning peptide library made up of hexapeptides to identify antigenic determinants recognized by mAbs. The 120 mixtures in the hexapeptide library are tested for their inhibitory activity in a competitive ELISA. The second protocol uses a decapeptide library to identify T cell peptide ligands. The 200 mixtures of the decapeptide library are tested for their ability to induce T cell activation. Support protocols cover optimization of the assay conditions for each mAb or T cell, to achieve the best level of sensitivity and reproducibility, and preparation of a hexapeptide library, along with deconvolution approaches.

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Section: Commentarymentioning

confidence: 99%