Single-cell analysis by use of ICP-MS

Abstract

This critical review article is highlighting the fundamentals, instrumentation, and most recent trends of single-cell analysis by use of inductively coupled plasma-mass spectrometry (ICP-MS). It is shown that metals and...

Beyond Single-Cell Analysis of Metallodrugs by ICP-MS: Targeting Cellular Substructures

“…When performing quantitative analysis of metallodrugs by means of ICP-MS following digestion of targeted cells, this information is typically lost. However, SC-ICP-MS overcomes these conceptual shortcomings of standard analysis approaches, since it is capable to detect metal (e.g., Pt) contents of individual cells and therefore permits tracking and quantification of metallodrugs in biological samples [27]. The use of SC-ICP-MS allowed both, to determine the cisPt content of single cells and to quantify the cisPt present in individual extracted nuclei.…”

“…In this study, SC-ICP-MS has proven to be a robust alternative to conventional ICP-MS for the quantification of intracellular platinum. By applying this method, the cells dissolved in water and PBS remain intact, thus preventing the loss of intracellular metal due to membrane permeabilization [27]. In this present study, the accumulation of cisPt is quantified by means of SC-ICP-MS in sensitive, resistant, and for the first time in the nuclei of A24 cells.…”

Elemental analysis by Metallobalance provides a complementary support layer over existing blood biochemistry panel-based cancer risk assessment

“…For new cancer screening methods, it is common to ponder whether the test itself provides any added value over existing ones; while ICP-MS has been extensively deployed in the field of chemistry and engineering, the as-is deployment of such an instrumentation technique in healthcare as a direct conduit for cancer screening may raise eyebrows. However, ICP-MS's notable resistance to specimen degradation, when compared to other analytical tools, has led itself to be utilized in various degradation monitoring studies (Kannamkumarath et al, 2004;Heuckeroth et al, 2021;Brunnbauer et al, 2020), including biological specimens (Hann et al, 2003;Theiner et al, 2015;Neumann et al, 2020;Meyer et al, 2018;Theiner et al, 2020) even as far as single-cell-based studies. The high sensitivity of elemental analysis also ensures high levels of reproducibility; since MB can monitor simultaneously 17 elemental levels, the probability of all of the elements being affected by external factors is likely lower than that of a single or even a small panel of cancer biomarkers.…”

Nanoparticles in analytical laser and plasma spectroscopy – a review of recent developments in methodology and applications

“…The exploitation of spICP-MS for bioanalytics is also advantageous. 381,382 For example, it was utilized for the individual detection of cells that is generally referred to as single cell ICP-MS (scICP-MS) in the literature. As cells are signicantly larger than NPs, special sample introduction tools were developed and applied for scICP-MS to boost cell transport efficiency.…”