Single-cell analysis by ambient mass spectrometry

Yang, Yunyun; Huang, Yanying; Wu, Junhui; Liu, Ning; Deng, Jiewei; Luan, Tiangang

doi:10.1016/j.trac.2017.02.009

Cited by 80 publications

(70 citation statements)

References 88 publications

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“…[46] Here,wewill focus on some of these technologies based on two types of sampling approaches,n amely,probe sampling and laser ablation. Minimizing perturbations is important because it helps to preserve metabolite, lipid, and peptide composition.…”

Section: Ambient Ionization Methods Offer Low Perturbationmentioning

confidence: 99%

“…Fore xample,i on beams can be routinely focused to around 100 nm, whereas the spot size produced from laser beams typically exceeds the diffraction limit. In contrast, matrix-assisted laser desorption/ionization (MAL-DI) MS [43] and matrix-free laser desorption/ionization (LDI) MS, [44,45] which have also been demonstrated for single-cell analysis,sample larger volumes and typically provide alarger molecular coverage.S imilarly,l ow-throughput ambient ionization MS techniques allow chemical analysis with high molecular coverage, [46] whereas mass cytometry is ah ighthroughput single-cell analysis method capable of distinguish-Compositional diversity is afundamental property in cell populations. [40][41][42] SIMS spectra, however, primarily contain information about low mass species (m/z < 2000).…”

Section: Introductionmentioning

confidence: 99%

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Single‐Cell Mass Spectrometry Approaches to Explore Cellular Heterogeneity

2018

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Compositional diversity is a fundamental property in cell populations. Single-cell analysis promises new insight into this cellular heterogeneity on the genomic, transcriptomic, proteomic, and metabolomic levels. Mass spectrometry (MS) is a label-free technique that enables the multiplexed analysis of proteins, peptides, lipids, and metabolites in individual cells. The abundances of these molecular classes are correlated with the physiological states and environmental responses of the cells. In this Minireview, we discuss recent advances in single-cell MS techniques with an emphasis on sampling and ionization methods developed for volume-limited samples. Strategies for sample treatment, separation methods, and data analysis require special considerations for single cells. Ongoing analytical challenges include subcellular heterogeneity, non-normal statistical distributions of cellular properties, and the need for high-throughput, high molecular coverage and minimal perturbation.

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Section: Ambient Ionization Methods Offer Low Perturbationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Single‐Cell Mass Spectrometry Approaches to Explore Cellular Heterogeneity

2018

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“…Single cell metabolomics and peptidomics aim to recognize the similarities and distinctions between individual cells by linking their chemical dynamic profiles to cellular fate, function, homeostatic balance, and other biological phenomena . Among the bioanalytical techniques used in single cell metabolomic and peptidomic investigations, mass spectrometry (MS) is at the forefront, owing to its high analyte coverage, low limits of detection (LOD), versatile analyte sampling methods, and unique ability to be coupled with in situ or off‐line orthogonal characterizations ,. MS imaging (MSI) focuses on direct analysis of tissue sections to determine the relative abundance and spatial distribution of analytes in tissue sections ,.…”

Section: Introductionmentioning

confidence: 99%

“…[9][10][11][12] Among the bioanalytical techniques used in single cell metabolomic and peptidomic investigations, mass spectrometry (MS) is at the forefront, owing to its high analyte coverage, low limits of detection (LOD), versatile analyte sampling methods, and unique ability to be coupled with in situ or offline orthogonal characterizations. [9,[11][12][13][14][15][16][17][18][19][20] MS imaging (MSI) focuses on direct analysis of tissue sections to determine the relative abundance and spatial distribution of analytes in tissue sections. [11,[21][22][23][24][25] In addition, multimodal MSI approaches have facilitated detection of diverse analyte classes from the same samples by utilizing different matrices and sequential tissue imaging.…”

Section: Introductionmentioning

confidence: 99%

Optically Guided Single Cell Mass Spectrometry of Rat Dorsal Root Ganglia to Profile Lipids, Peptides and Proteins

et al. 2018

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The mammalian dorsal root ganglia (DRG) are located on the dorsal roots of the spinal nerves and contain cell bodies of primary sensory neurons. DRG cells have been classified into subpopulations based on their size, morphology, intracellular markers, response to stimuli, and neuropeptides. To understand the connections between DRG chemical heterogeneity and cellular function, we performed optically guided, high-throughput single cell profiling using sequential matrix-assisted laser desorption/ionization mass spectrometry (MS) to detect lipids, peptides, and several proteins in individual DRG cells. Statistical analysis of the resulting mass spectra allows stratification of the DRG population according to cellular morphology and, presumably, major cell types. A subpopulation of small cells contained myelin proteins, which are abundant in Schwann cells, and mass spectra of several larger cells contained peaks matching neurofilament, vimentin, myelin basic protein S, and thymosin beta proteins. Of the over 1000 cells analyzed, approximately 78 % produced putative peptide-rich spectra, allowing the population to be classified into three distinct cell types. Two signals with m/z 4404 and 5487 were exclusively observed in a cell type, but could not be matched to results of our previous liquid chromatography-MS analyses.

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“…Dagegen beproben die matrixgestützte Laser‐Desorptions/Ionisations(MALDI)‐MS und die matrixfreie Laser‐Desorptions/Ionisations(LDI)‐MS, die auch für die Einzelzellanalyse angewendet wurden, größere Volumina und erfassen normalerweise ein größeres Molekülspektrum. Auf ähnliche Weise ermöglichen Atmosphärendruck‐Ionisations‐MS‐Verfahren mit geringem Durchsatz eine chemische Analyse mit großer Molekülbandbreite, wohingegen die Massenzytometrie eine Hochdurchsatz‐Einzelzellanalyse ist, die begrenzt durch die Zahl der verfügbaren Metallmarkierungen wenige Dutzend gezielte Analyten unterscheiden kann.…”

Section: Introductionunclassified

Einzelzell‐Massenspektrometrie zur Untersuchung zellulärer Heterogenität

Zhang

Vertes

2018

Angewandte Chemie

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Die Diversität der Zusammensetzung ist eine fundamentale Eigenschaft von Zellpopulationen. Neue Einblicke in diese zelluläre Heterogenität auf Genom‐, Transkriptom‐, Proteom‐ und Metabolomniveau verspricht die Einzelzellanalyse. Die Massenspektrometrie (MS) ist ein markierungsfreies Verfahren, das die Multiplex‐Analyse von Proteinen, Peptiden, Lipiden und Metaboliten in Einzelzellen ermöglicht. Die Häufigkeiten dieser Verbindungsklassen werden mit den physiologischen Zuständen und den Reaktionen der Zellen auf Umgebungsänderungen korreliert. Dieser Kurzaufsatz beschreibt neue Fortschritte bei den Einzelzell‐MS‐Techniken mit dem Schwerpunkt auf Entnahme‐ und Ionisationsmethoden, die für volumenbegrenzte Proben entwickelt wurden. Bei Einzelzellen erfordern die Strategien zur Probenbehandlung, die Trennmethoden und die Datenanalyse besondere Überlegungen. Aktuelle analytische Herausforderungen betreffen unter anderem die subzelluläre Heterogenität, nicht normale statistische Verteilungen von Zelleigenschaften sowie die Notwendigkeit von Hochdurchsatzanwendungen, einem breiten Molekülspektrum und minimaler Störung.

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Single-cell analysis by ambient mass spectrometry

Cited by 80 publications

References 88 publications

Single‐Cell Mass Spectrometry Approaches to Explore Cellular Heterogeneity

Single‐Cell Mass Spectrometry Approaches to Explore Cellular Heterogeneity

Optically Guided Single Cell Mass Spectrometry of Rat Dorsal Root Ganglia to Profile Lipids, Peptides and Proteins

Einzelzell‐Massenspektrometrie zur Untersuchung zellulärer Heterogenität

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