Single-cell activity and synchronous bursting in the rat hippocampus during waking behavior and sleep

Suzuki, Shinya; Smith, Grant K.

doi:10.1016/0014-4886(85)90266-3

Cited by 48 publications

(40 citation statements)

References 33 publications

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“…These two baseline values of the 5-HT-depleted rats were significantly higher than those of control rats (event The other parameters, spikes/event and CS%, were not different between the two groups (spikes/event: control 2.02 Ϯ 0.05 vs. PCPA 2.00 Ϯ 0.07, CS%: control 65.97 Ϯ 3.54% vs. PCPA 62.90 Ϯ 3.21%). The event rate of the control was comparable with the rates previously reported during this awake immobile behavioral state (Suzuki and Smith 1985;Tada et al 1999). …”

Section: Effect Of a 5-ht Depletion By Pcpa On Baseline Firing Activisupporting

confidence: 88%

“…Similarly, PCPA and NAN-190 are expected to reduce the firing activity through increasing wakefulness (Li and Satinoff 1992;Neckelmann et al 1996) whereas PCPA increased and NAN-190 unchanged the firing activity (Figure 2) (Tada et al 1999). Moreover, it has been demonstrated that a selective 5-HT 1A agonist 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT) increases the wakefulness (Monti and Hantos 1994) and decreases the firing activity (Tada et al 1999), which is consistent with the behavioral correlates (Suzuki and Smith 1985). Taken together, the actual influences of the drugs on the firing activity were extremely different from the expectable indirect influences through the effects on the behavioral states.…”

Section: Discussionmentioning

confidence: 86%

“…Previous studies have demonstrated that PCPA and NAN-190 decrease slow wave sleep and increase wakefulness (Li and Satinoff 1992;Neckelmann et al 1996), and that ritanserin increases slow wave sleep and decreases waking (Bjorvatn and Ursin 1990;Silhol et al 1992). Suzuki and Smith (1985) have shown that the pyramidal neurons are most active during slow wave sleep, slightly less active during a quiet awake state, and least active during an active awake condition and paradoxical sleep. Thus, one might assume that the present observations reflect indirect influences of the tested drugs through their direct effects on the states of the animals.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, one might assume that the present observations reflect indirect influences of the tested drugs through their direct effects on the states of the animals. Given the correlation between the behavioral states and the firing activity (Suzuki and Smith 1985), ritanserin is expected to increase the firing activity since this drug could increase slow wave sleep and decrease wakefulness (Bjorvatn and Ursin 1990;Silhol et al 1992). However, ritanserin did not change the firing activity (data not shown).…”

Section: K Kasamo Et Almentioning

confidence: 93%

“…The criteria were generated on the basis of the previous studies (Fox and Ranck 1975;Suzuki and Smith 1985). Recordings with a low signal-to-noise ratio ( Ͻ 5:1) were not employed for data analysis.…”

Section: Identification and Isolation Of Ca1 Pyramidal Neuronsmentioning

confidence: 99%

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Endogenous 5-HT Tonically Inhibits Spontaneous Firing Activity of Dorsal Hippocampus CA1 Pyramidal Neurons Through Stimulation of 5-HT1A Receptors in Quiet Awake Rats In Vivo Electrophysiological Evidence

Kasamo

2001

Neuropsychopharmacology

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The present study was performed to examine an overall effect of endogenous serotonin (5-HT) on the spontaneous firing activity of the dorsal hippocampus CA1 pyramidal neurons in quiet awake rats. A selective 5-HT 1A antagonist N-[2-[4-(2-methoxyphenyl)-1-piperazinyl]ethyl]-N-(2-pyridinyl)cyclohexanecarboxamide (WAY-100635: 0.03-0.2 mg/kg, s.c.) It has been shown that there are at least 14 serotonin (5-HT; 5-hydroxytryptamine) receptor subtypes over seven distinct receptor subfamilies. Among these seven receptor subfamilies, only the 5-HT 1 subfamily is inhibitory whereas the others are excitatory (see Hoyer et al. 1994; Glennon and Dukat 1995 for reviews). Several in situ hybridization studies have demonstrated that ten of these 14 receptor subtypes are detectable over the CA1 region of the rat hippocampus (see Andrade 1998 for review).In accordance with the distribution and exuberance of 5-HT receptors expressed in this area, several in vitro electrophysiological studies have demonstrated multiple actions of 5-HT on the excitability of the CA1 pyramidal neurons. These are the direct inhibitory and exci- 142 K. Kasamo et al. N EUROPSYCHOPHARMACOLOGY 2001 -VOL . 24 , NO . 2 tatory actions through stimulating 5-HT 1A and 5-HT 4 receptors on the pyramidal neurons, respectively. In addition, via inhibitory interneurons, 5-HT indirectly decreases and increases the excitability through stimulating 5-HT 2 and 5-HT 3 , and 5-HT 1A receptors, respectively (see Hoyer et al. 1994; Aghajanian 1995; Andrade 1998 for reviews). Given these extremely diverse actions of 5-HT demonstrated in vitro , endogenous 5-HT should produce multiple actions simultaneously on the excitability of the CA1 pyramidal neurons in vivo. These in vitro data have been obtained with the preparations isolated from various potentially confounding factors, such as the effects of activation of other receptor systems. Contrary, in vivo cells in the brain are exposed continuously to the various neurotransmitter, neuromodulator and hormonal stimuli. In any cell, these stimuli are integrated spatially and temporally. Furthermore, the intracellular signaling cascades are extensively interconnected. Moreover, even in vivo , the use of anesthesia drastically reduces the 5-HT tone (Jacobs 1985; as well as the spontaneous firing activity of the hippocampus pyramidal neurons (Blier et al. 1993). The reduction in the firing activity indicates that the use of anesthesia per se markedly decreases the excitability of hippocampal pyramidal neurons. Thus, an overall action of endogenous 5-HT on the firing activity of the pyramidal neurons in awake rats is unpredictable solely basing on the data obtained from in vivo studies with anesthetized animals and from in vitro experiments. Therefore, using awake animals is essential to scrutinize the influence of endogenous 5-HT. To our knowledge, such studies examining the net effect of endogenous 5-HT on cellular activity in the brain of awake rats have not been performed so far.Thus, the present study was carried out to...

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Section: Effect Of a 5-ht Depletion By Pcpa On Baseline Firing Activisupporting

confidence: 88%

Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: K Kasamo Et Almentioning

confidence: 93%

Section: Identification and Isolation Of Ca1 Pyramidal Neuronsmentioning

confidence: 99%

See 3 more Smart Citations

Endogenous 5-HT Tonically Inhibits Spontaneous Firing Activity of Dorsal Hippocampus CA1 Pyramidal Neurons Through Stimulation of 5-HT1A Receptors in Quiet Awake Rats In Vivo Electrophysiological Evidence

Kasamo

2001

Neuropsychopharmacology

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Single neuron burst firing in the human hippocampus during sleep

et al. 2002

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Although there are numerous non-primate studies of the single neuron correlates of sleep-related hippocampal EEG patterns, very limited hippocampal neuronal data are available for correlation with human sleep. We recorded human hippocampal single neuron activity in subjects implanted with depth electrodes required for medical diagnosis and quantitatively evaluated discharge activity from each neuron during episodes of wakefulness (Aw), combined stage 3 and 4 slow-wave sleep (SWS), and rapid eye movement (REM) sleep. The mean firing rate of the population of single neurons was significantly higher during SWS and Aw compared with REM sleep (p = 0.002; p < 0.0001). In addition, burst firing was significantly greater during SWS compared with Aw (p = 0.001) and REM sleep (p < 0.0001). The synchronized state of SWS and associated high-frequency burst discharge found in human hippocampus may subserve functions similar to those reported in non-primate hippocampus that require burst firing to induce synaptic modifications in hippocampal circuitry and in hippocampal projections to neocortical targets that participate in memory consolidation.

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Endogenous 5‐HT inhibits firing activity of hippocampal CA₁ pyramidal neurons during conditioned fear stress‐induced freezing behavior through stimulating 5‐HT_1A receptors

et al. 2004

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This study examines the activity of hippocampal CA, pyramidal neurons during conditioned fear stress (CFS)-induced freezing behavior in unanesthetized, unrestrained rats. The firing frequency of hippocampal CA1 pyramidal neurons was significantly decreased when conditioned rats exhibited freezing behavior. Firing frequency returned to the baseline after freezing behavior disappeared. The selective 5-hydroxytryptamine (5-HT)1A antagonists, N-[2-[4-(2-methoxyphenyl)-1-piperazinyl]ethyl]-N-(2-pyridinyl)cyclohexanecarboxamide (WAY-100635), and N-tert-butyl-3-[4-(2-methoxyphenyl)piperazine-1-yl]-2-phenylpropamide (WAY-100135) and 5-HT depletion with parachlorophenylalanine (PCPA) completely abolished the decrease in firing frequency during CFS-induced freezing behavior. These results suggested that endogenous 5-HT inhibited the firing activity of hippocampal CA1 pyramidal neurons during CFS-induced freezing behavior mainly through stimulating 5-HT1A receptors.

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Single-cell activity and synchronous bursting in the rat hippocampus during waking behavior and sleep

Cited by 48 publications

References 33 publications

Endogenous 5-HT Tonically Inhibits Spontaneous Firing Activity of Dorsal Hippocampus CA1 Pyramidal Neurons Through Stimulation of 5-HT1A Receptors in Quiet Awake Rats In Vivo Electrophysiological Evidence

Endogenous 5-HT Tonically Inhibits Spontaneous Firing Activity of Dorsal Hippocampus CA1 Pyramidal Neurons Through Stimulation of 5-HT1A Receptors in Quiet Awake Rats In Vivo Electrophysiological Evidence

Single neuron burst firing in the human hippocampus during sleep

Endogenous 5‐HT inhibits firing activity of hippocampal CA₁ pyramidal neurons during conditioned fear stress‐induced freezing behavior through stimulating 5‐HT_1A receptors

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Single-cell activity and synchronous bursting in the rat hippocampus during waking behavior and sleep

Cited by 48 publications

References 33 publications

Endogenous 5-HT Tonically Inhibits Spontaneous Firing Activity of Dorsal Hippocampus CA1 Pyramidal Neurons Through Stimulation of 5-HT1A Receptors in Quiet Awake Rats In Vivo Electrophysiological Evidence

Endogenous 5-HT Tonically Inhibits Spontaneous Firing Activity of Dorsal Hippocampus CA1 Pyramidal Neurons Through Stimulation of 5-HT1A Receptors in Quiet Awake Rats In Vivo Electrophysiological Evidence

Single neuron burst firing in the human hippocampus during sleep

Endogenous 5‐HT inhibits firing activity of hippocampal CA1 pyramidal neurons during conditioned fear stress‐induced freezing behavior through stimulating 5‐HT1A receptors

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Endogenous 5‐HT inhibits firing activity of hippocampal CA₁ pyramidal neurons during conditioned fear stress‐induced freezing behavior through stimulating 5‐HT_1A receptors