Single carbon metabolism – A new paradigm for microbial bioprocesses?

Baumschabl, Michael; Ata, Özge; Mattanovich, Diethard

doi:10.1016/j.synbio.2024.03.003

Cited by 3 publications

(1 citation statement)

References 104 publications

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“…Naturally, this has prompted greater technological investment in alternative substrates, aiming for a neutral carbon footprint and sidestepping ethical implications. Sustainable resources of interest include various waste products such as crude glycerol [ 11 ], lignocellulosic biomass [ 12 ], food waste [ 13 ] and more unconventionally single-carbon (C1) substrates derived from CO 2 [ 14 ]. Valorising CO 2 and its derived C1 substrates is a growing area of interest, being developed in various biotechnological [ 4 , 15 – 17 ] and chemical fields [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Efficient production of itaconic acid from the single-carbon substrate methanol with engineered Komagataella phaffii

Severinsen,

Bachleitner,

Modenese

et al. 2024

Biotechnol Biofuels

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Background Amidst the escalating carbon dioxide levels resulting from fossil fuel consumption, there is a pressing need for sustainable, bio-based alternatives to underpin future global economies. Single-carbon feedstocks, derived from CO2, represent promising substrates for biotechnological applications. Especially, methanol is gaining prominence for bio-production of commodity chemicals. Results In this study, we show the potential of Komagataella phaffii as a production platform for itaconic acid using methanol as the carbon source. Successful integration of heterologous genes from Aspergillus terreus (cadA, mttA and mfsA) alongside fine-tuning of the mfsA gene expression, led to promising initial itaconic acid titers of 28 g·L−1 after 5 days of fed-batch cultivation. Through the combined efforts of process optimization and strain engineering strategies, we further boosted the itaconic acid production reaching titers of 55 g·L−1 after less than 5 days of methanol feed, while increasing the product yield on methanol from 0.06 g·g−1 to 0.24 g·g−1. Conclusion Our results highlight the potential of K. phaffii as a methanol-based platform organism for sustainable biochemical production.

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Section: Introductionmentioning

confidence: 99%

Efficient production of itaconic acid from the single-carbon substrate methanol with engineered Komagataella phaffii

Severinsen,

Bachleitner,

Modenese

et al. 2024

Biotechnol Biofuels

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Anchoring Bimetal Zinc–Aluminum Sites on Nitrogen‐Doped Carbon Catalyst for Efficient Conversion of CO₂ Into Cyclic Carbonates

Li,

Wang,

Huang

et al. 2024

ChemistrySelect

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The design and synthesis of efficient carbon‐based materials incorporating multifunctional sites is prospective technology for converting carbon dioxide (CO2) into high‐value chemicals. Here, nitrogen‐doped carbon catalysts (ZnAl‐NC) with bimetal zinc–aluminum sites were synthesized using nitrogen‐rich polyaniline (PANI) and ZnAl‐LDH through hydrothermal and in situ pyrolysis methods. ZnAl‐NC‐400, in the presence of tetrabutylammonium bromide (TBAB), exhibited excellent catalytic activity without solvent, achieving high yields (97.2% or 98.5%) in the cycloaddition of CO2 with propylene oxide (PO) under traditional or mild conditions. NH3‐TPD and CO2‐TPD results indicated that the enhanced catalytic activity resulted from the acid–base synergistic effect, involving Lewis acid sites from Zn and Al species and abundant Lewis base sites from the PANI matrix. The impact of calcination temperature on catalyst morphology, structure, and performance was investigated, along with catalyst recycling and adaptability to various epoxides. This work presents an efficient catalyst for CO2 conversion and suggests new approaches for designing catalytic sites in carbon‐based catalysts for CO2 capture and utilization.

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Efficient production of itaconic acid from the single carbon substrate methanol with engineeredKomagataella phaffii

Severinsen,

Bachleitner,

Modenese

et al. 2024

Preprint

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Amidst the escalating carbon dioxide levels resulting from fossil fuel consumption, there is a pressing need for sustainable, bio-based alternatives to underpin future global economies. Single carbon feedstocks, derived from CO2, represent promising substrates for biotechnological applications. Especially methanol is gaining prominence for bio-production of commodity chemicals. In this study, we show the potential of Komagataella phaffii as a production platform for itaconic acid using methanol as the carbon source. Successful integration of heterologous genes from Aspergillus terreus (cadA, mttA and mfsA) alongside fine-tuning of the mfsA gene expression, led to promising initial itaconic acid titers of 28 g L-1 after five days of fed-batch cultivation. Through the combined efforts of process optimization and strain engineering strategies we further boosted the itaconic acid production reaching titers of 55 g L-1 after less than five days of methanol feed, whilst increasing the product yield on methanol from 0.06 g g-1 to 0.24 g g-1. Our results highlight the potential of K. phaffii as a methanol-based platform organism for sustainable biochemical production.

show abstract

Single carbon metabolism – A new paradigm for microbial bioprocesses?

Cited by 3 publications

References 104 publications

Efficient production of itaconic acid from the single-carbon substrate methanol with engineered Komagataella phaffii

Efficient production of itaconic acid from the single-carbon substrate methanol with engineered Komagataella phaffii

Anchoring Bimetal Zinc–Aluminum Sites on Nitrogen‐Doped Carbon Catalyst for Efficient Conversion of CO₂ Into Cyclic Carbonates

Efficient production of itaconic acid from the single carbon substrate methanol with engineeredKomagataella phaffii

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Single carbon metabolism – A new paradigm for microbial bioprocesses?

Cited by 3 publications

References 104 publications

Efficient production of itaconic acid from the single-carbon substrate methanol with engineered Komagataella phaffii

Efficient production of itaconic acid from the single-carbon substrate methanol with engineered Komagataella phaffii

Anchoring Bimetal Zinc–Aluminum Sites on Nitrogen‐Doped Carbon Catalyst for Efficient Conversion of CO2 Into Cyclic Carbonates

Efficient production of itaconic acid from the single carbon substrate methanol with engineeredKomagataella phaffii

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About

Anchoring Bimetal Zinc–Aluminum Sites on Nitrogen‐Doped Carbon Catalyst for Efficient Conversion of CO₂ Into Cyclic Carbonates