Single bovine sperm sex typing by amelogenin nested PCR

Colley, Adam J.; Buhr, M.M.; Golovan, Serguei P.

doi:10.1016/j.theriogenology.2008.05.060

Cited by 12 publications

(15 citation statements)

References 21 publications

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“…Moreover, the detection of transcripts of other genes such as KRT8 and AMELX and AMELY are somewhat surprising. However, some members of the superfamily of keratins are involved in the shape of the sperm structure (Hinsch et al, 2003;Kierszenbaum, 2002;Kierszenbaum et al, 2007), while amelogenin seems to play a role in male fertility and genetic divergence in X-Y pairing (Colley et al, 2008;Matsuda et al, 1991).…”

Section: Microarray Studymentioning

confidence: 99%

Transcriptome analysis of bull spermatozoa: implications for male fertility

Feugang

Rodríguez-Osorio

Kaya³

et al. 2010

Reproductive BioMedicine Online

110

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Spermatozoa deliver more than the paternal genome into the oocyte; they also carry remnant messenger RNA from spermatogenesis. The RNA profiles of spermatozoa from high-fertility and a low-fertility Holstein bulls were analysed using Affymetrix bovine genechips. A total of 415 transcripts out of approximately 24,000 were differentially detected in spermatozoa collected from both bulls (fold change > or =2.0; P<0.01). These transcripts were associated with different cellular functions and biological processes. Spermatozoa from high-fertility bulls contained higher concentrations of transcripts for membrane and extracellular space protein locations, while spermatozoa from the low-fertility bulls were deficient of transcripts for transcriptional and translational factors. Quantitative real-time PCR was used on three low-fertility and four high-fertility bulls to validate the microarray data. Two highly represented transcripts in the microarray analysis (protamine 1 and casein beta 2) were validated, as well as a third transcript (thrombospondin receptor CD36 molecule) that showed a lower concentration in low-fertility bulls. This study presents the global analysis of spermatozoa originating from bulls with opposite fertility. These results provide some specific transcripts in spermatozoa that could be associated with bull fertility.

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Section: Microarray Studymentioning

confidence: 99%

Transcriptome analysis of bull spermatozoa: implications for male fertility

Feugang

Rodríguez-Osorio

Kaya³

et al. 2010

Reproductive BioMedicine Online

110

View full text Add to dashboard Cite

show abstract

“…The multiplex real-time PCR technique as developed in this study yielded accurate assessment of the sperm sex ratio in both sorted and unsorted semen. Although the accurate assessment of the ratio can be obtained with other techniques such as flow cytometry analysis (Garner et al, 2013), individual sperm-PCR (Colley et al, 2008; Wang et al, 2011), simplex real-time PCR (Parati et al, 2006), the multiplex method as reported in this study is simpler, faster, and more cost-effective to perform with only a single reaction tube required for each semen sample. In conclusion, the multiplex real-time PCR assay could be used as an alternative method to identify the sex ratio of the sperm in bovine semen.…”

Section: Discussionmentioning

confidence: 99%

“…However, this technique is complicated, laborious, time-consuming, and requires highly skilled technicians, which limits its use. Polymerase chain reaction (PCR) techniques for detecting DNA sequences on sex chromosomes have been reported which can be used to identify the sex of individual spermatozoa and sex ratios of spermatozoa in semen (Colley et al, 2008; Wang et al, 2011). However, accurate determination of the sex ratio by single-sperm PCR requires analysis of a large number of individual sperm, which is laborious and expensive.…”

Section: Introductionmentioning

confidence: 99%

Determination of Sperm Sex Ratio in Bovine Semen Using Multiplex Real-time Polymerase Chain Reaction

Khamlor

Pongpiachan

Sangsritavong

et al. 2014

Asian Australas. J. Anim. Sci

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Gender selection is important in livestock industries; for example, female calves are required in the dairy industry. Sex-sorted semen is commonly used for the production of calves of the desired gender. However, assessment of the sex ratio of the sorted semen is tedious and expensive. In this study, a rapid, cost effective and reliable method for determining the sex ratio was developed using a multiplex real-time polymerase chain reaction (PCR) assay. In this assay, the X and Y chromosome-specific markers, i.e., bovine proteolipid protein (PLP) gene and sex-determining region Y (SRY) were simultaneously quantified in a single tube. The multiplex real-time PCR assay was shown to have high amplification efficiencies (97% to 99%) comparable to the separated-tube simplex real-time PCR assay. The results obtained from both assays were not significantly different (p>0.05). The multiplex assay was validated using reference DNA of known X ratio (10%, 50%, and 90%) as templates. The measured %X in semen samples were the same within 95% confidence intervals as the expected values, i.e., >90% in X-sorted semen, <10% in Y-sorted semen and close to 50% in the unsorted semen. The multiplex real-time PCR assay as shown in this study can thus be used to assess purity of sex-sorted semen.

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“…Specific DNA sequences on X- and Y-sperm have been reported which can be used to identify the sex of individual sperm and sex ratios of sperm in semen sample [65,66]. Accurate determination of the sex ratio using single sperm PCR necessitates analysis of a large number of individual sperm.…”

Section: Different Approaches Of Immunological Sperm Sexingmentioning

confidence: 99%

An immunological approach of sperm sexing and different methods for identification of X- and Y-chromosome bearing sperm

et al. 2017

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Separation of X- and Y-chromosome bearing sperm has been practiced for selection of desired sex of offspring to increase the profit in livestock industries. At present, fluorescence-activated cell sorter is the only successful method for separation of X- and Y-chromosome bearing sperm. This technology is based on the differences in DNA content between these two types of sperm and has been commercialized for bovine sperm. However, this technology still has problems in terms of high economic cost, sperm damage, and lower pregnancy rates compared to unsorted semen. Therefore, an inexpensive, convenient, and non-invasive approach for sperm sexing would be of benefit to agricultural sector. Within this perspective, immunological sperm sexing method is one of the attractive choices to separate X- and Y-chromosome bearing sperm. This article reviews the current knowledge about immunological approaches, viz., H-Y antigen, sex-specific antigens, and differentially expressed proteins for sperm sexing. Moreover, this review also highlighted the different methods for identification of X- and Y-sperm.

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Single bovine sperm sex typing by amelogenin nested PCR

Cited by 12 publications

References 21 publications

Transcriptome analysis of bull spermatozoa: implications for male fertility

Transcriptome analysis of bull spermatozoa: implications for male fertility

Determination of Sperm Sex Ratio in Bovine Semen Using Multiplex Real-time Polymerase Chain Reaction

An immunological approach of sperm sexing and different methods for identification of X- and Y-chromosome bearing sperm

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