Single-axon level automatic segmentation and feature extraction from immuhistochemical images of peripheral nerves

Tóth, Viktor; Jayaprakash, Naveen; Abbas, Adam; Khan, Ariba; Zanos, Stavros; Zanos, Theodoros P.

doi:10.1109/embc44109.2020.9175974

Cited by 5 publications

(1 citation statement)

References 15 publications

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“…Furthermore, high-content analysis and implementation of segmentation methods helps to minimize selection-bias by the examiner. Segmentation techniques and analysis applying deep learning algorithms have been applied not only in SLML applications, but also on rodent and porcine peripheral nerve and human healthy CNS cross sections (56,(83)(84)(85)(86). Here, we could implement automated segmentation on murine healthy samples, and integrate image segmentation in the analysis of human histopathological samples, though not in a fully automated-manner.…”

Section: Super-resolution Imaging Visualizes the Link Of Paranodalmentioning

confidence: 99%

Super-resolution imaging pinpoints ultrastructural changes at the node of Ranvier in patients with polyneuropathy

Appeltshauser

Linke

Heil

et al. 2022

Preprint

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The peripheral node of Ranvier is the key element in saltatory conduction along myelinated axons, but its ultrastructural protein arrangement and interactions remain elusive in humans. To assess both physiological protein arrangement and pathological ultrastructural changes at the paranodal region of the node of Ranvier, we implement the super resolution microscopy method direct Stochastic Optical Reconstruction Microscopy (dSTORM) assisted by high-content confocal microscopy on nerve biopsies of patients with polyneuropathies. We demonstrate that a ~190nm periodic arrangement of axonal cytoskeletal proteins is conserved in humans, but impaired at the paranode in polyneuropathy. In patients with polyneuropathy, the periodic protein distances of the paranodal adhesion molecules Caspr-1 and neurofascin-155 were severely elongated, implying a lateral decoiling and partial loss of the axoglial complex at the node of Ranvier. Dual-color colocalization analysis of the super-resolved dSTORM images revealed that the axoglial complex itself remained closely attached even under pathological conditions, but was detached from its cytoskeletal axonal anchor. Correspondingly, high-content confocal analysis of nodes of Ranvier showed that paranodal elongation occurred especially in acute and severe axonal neuropathy. Our findings show that super-resolution techniques open the possibility to identify underlying nanoscale pathology in polyneuropathies, with possible future use in diagnostic assessment.

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Section: Super-resolution Imaging Visualizes the Link Of Paranodalmentioning

confidence: 99%

Super-resolution imaging pinpoints ultrastructural changes at the node of Ranvier in patients with polyneuropathy

Appeltshauser

Linke

Heil

et al. 2022

Preprint

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Organ- and function-specific anatomical organization of vagal fibers supports fascicular vagus nerve stimulation

Jayaprakash¹,

Song²,

Tóth³

et al. 2023

Brain Stimulation

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Towards enhanced functionality of vagus neuroprostheses through in silico optimized stimulation

Ciotti,

John,

Katic Secerovic

et al. 2024

Nat Commun

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Bioelectronic therapies modulating the vagus nerve are promising for cardiovascular, inflammatory, and mental disorders. Clinical applications are however limited by side-effects such as breathing obstruction and headache caused by non-specific stimulation. To design selective and functional stimulation, we engineered VaStim, a realistic and efficient in-silico model. We developed a protocol to personalize VaStim in-vivo using simple muscle responses, successfully reproducing experimental observations, by combining models with trials conducted on five pigs. Through optimized algorithms, VaStim simulated the complete fiber population in minutes, including often omitted unmyelinated fibers which constitute 80% of the nerve. The model suggested that all Aα-fibers across the nerve affect laryngeal muscle, while heart rate changes were caused by B-efferents in specific fascicles. It predicted that tripolar paradigms could reduce laryngeal activity by 70% compared to typically used protocols. VaStim may serve as a model for developing neuromodulation therapies by maximizing efficacy and specificity, reducing animal experimentation.

show abstract

Single-axon level automatic segmentation and feature extraction from immuhistochemical images of peripheral nerves

Cited by 5 publications

References 15 publications

Super-resolution imaging pinpoints ultrastructural changes at the node of Ranvier in patients with polyneuropathy

Super-resolution imaging pinpoints ultrastructural changes at the node of Ranvier in patients with polyneuropathy

Organ- and function-specific anatomical organization of vagal fibers supports fascicular vagus nerve stimulation

Towards enhanced functionality of vagus neuroprostheses through in silico optimized stimulation

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