Single and combined effects of caffeine and salicylic acid on mussel Mytilus galloprovincialis: Changes at histomorphological, molecular and biochemical levels

Afsa, Sabrine; Cristaldi, Antonio; Giannetto, Alessia; Galati, Mariachiara; Billè, Barbara; Conti, Gea Oliveri; Mansour, Hédi Ben; Ferrante, Margherita; Cappello, Tiziana

doi:10.1016/j.etap.2023.104167

Cited by 5 publications

(3 citation statements)

References 131 publications

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“…For all the reasons mentioned above, in the present study, we investigate the effect that caffeine and MPs, alone and combined, exert on the gill of M. galloprovincialis. The study is based on the premise that gills accumulate caffeine, MPs, and other pollutants in their tissue, according to what is reported in the current scientific literature (Afsa et al, 2023;Capolupo et al, 2016, Cole et al, 2020De Marco et al, 2023). Consequently, the primary focus was on assessing the impact of the already demonstrated occurrence of MPs and caffeine in gills rather than directly measuring the presence itself.…”

Section: Mytilus Galloprovincialis Respiratory Apparatusmentioning

confidence: 99%

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

Impellitteri,

Briglia,

Porcino

et al. 2024

Microscopy Res & Technique

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In recent years, the presence of pharmaceuticals and microplastics (MPs) in aquatic ecosystems has raised concerns about their environmental impact. This study explores the combined effects of caffeine, a common pharmaceutical pollutant, and MPs on the marine mussel Mytilus galloprovincialis. Caffeine, at concentrations of 20.0 μg L−1, and MPs (1 mg L−1, 35–50 μm size range), was used to mimic real‐world exposure scenarios. Two hundred M. galloprovincialis specimens were divided into four groups: caffeine, MPs, Mix (caffeine + MPs), and Control. After a two‐week acclimation period, the mollusks were subjected to these pollutants in oxygen‐aerated aquariums under controlled conditions for 14 days. Histopathological assessments were performed to evaluate gill morphology. Cellular volume regulation and digestive gland cell viability were also analyzed. Exposure to caffeine and MPs induced significant morphological changes in M. galloprovincialis gills, including cilia loss, ciliary disk damage, and cellular alterations. The chitinous rod supporting filaments also suffered damage, potentially due to MP interactions, leading to hemocyte infiltration and filament integrity compromise. Hemocytic aggregation suggested an inflammatory response to caffeine. In addition, viability assessments of digestive gland cells revealed potential damage to cell membranes and function, with impaired cell volume regulation, particularly in the Mix group, raising concerns about nutrient metabolism disruption and organ function compromise. These findings underscore the vulnerability of M. galloprovincialis to environmental pollutants and emphasize the need for monitoring and mitigation efforts.Research Highlights The synergy of caffeine and microplastics (MPs) in aquatic ecosystems warrants investigation. MPs and caffeine could affect gill morphology of Mytilus galloprovincialis. Caffeine‐exposed cells had lower viability than the control group in the NR retention test. MPs and mix‐exposed cells struggled to recover their volume.

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Section: Mytilus Galloprovincialis Respiratory Apparatusmentioning

confidence: 99%

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

Impellitteri,

Briglia,

Porcino

et al. 2024

Microscopy Res & Technique

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“…Consequently, transcription modulation, along with changes in enzymatic activity, constitutes part of the early defense response activated to mitigate CAF-induced oxidative stress. Exposure to the CAF and SA mixture elicited significantly different transcriptional and biochemical responses compared to CAF alone, primarily due to the effects of SA [ 69 ].…”

Section: Effects Of Nsaids On Bivalvesmentioning

confidence: 99%

Non-Steroidal Anti-Inflammatory Drugs in the Aquatic Environment and Bivalves: The State of the Art

Pires,

Pereira,

Pena

et al. 2024

Toxics

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In recent years, contaminants of emerging concern have been reported in several environmental matrices due to advances in analytical methodologies. These anthropogenic micropollutants are detected at residual levels, representing an ecotoxicological threat to aquatic ecosystems. In particular, the pharmacotherapeutic group of non-steroidal anti-inflammatories (NSAIDs) is one of the most prescribed and used, as well as one of the most frequently detected in the aquatic environment. Bivalves have several benefits as a foodstuff, and also as an environment bioindicator species. Therefore, they are regarded as an ideal tool to assess this issue from both ecotoxicological and food safety perspectives. Thus, the control of these residues in bivalves is extremely important to safeguard environmental health, also ensuring food safety and public health. This paper aims to review NSAIDs in bivalves, observing their consumption, physicochemical characteristics, and mechanisms of action; their environmental occurrence in the aquatic environment and aquatic biota; and their effects on the ecosystem and the existent legal framework. A review of the analytical methodologies for the determination of NSAIDs in bivalves is also presented.

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“…Nevertheless, the formulation of optimal feeding diets for the wide variety of aquaculture fish species requires the implementation of different types of analyses to verify their impact on the health status of the specimens [ 251 ]. Among the different approaches employed [ 194 , 252 ], histomorphological assays represent a good biomarker for the assessment of the welfare of aquatic organisms [ 253 , 254 , 255 , 256 , 257 ] since it is able to promptly provide insights into the overall health status of individuals under examination. In particular, in the histological assays of fish guts, endpoints such as mucosal fold length (villi), muscle thickness (MT), and crypt depth (CD) might provide useful information regarding the efficiency of the gut in terms of nutrient absorption [ 223 ], while evaluation of the number of goblet cells and leukocytes may be useful for estimating the state of the immune system response, which is crucial for gut microbiota balance [ 222 ].…”

Section: Use Of Histomorphological Assays To Estimate the Quality Of ...mentioning

confidence: 99%

Histomorphological Changes in Fish Gut in Response to Prebiotics and Probiotics Treatment to Improve Their Health Status: A Review

De Marco,

Cappello,

Maisano

2023

Animals

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The gastrointestinal tract (GIT) promotes the digestion and absorption of feeds, in addition to the excretion of waste products of digestion. In fish, the GIT is divided into four regions, the headgut, foregut, midgut, and hindgut, to which glands and lymphoid tissues are associated to release digestive enzymes and molecules involved in the immune response and control of host-pathogens. The GIT is inhabited by different species of resident microorganisms, the microbiota, which have co-evolved with the host in a symbiotic relationship and are responsible for metabolic benefits and counteracting pathogen infection. There is a strict connection between a fish’s gut microbiota and its health status. This review focuses on the modulation of fish microbiota by feed additives based on prebiotics and probiotics as a feasible strategy to improve fish health status and gut efficiency, mitigate emerging diseases, and maximize rearing and growth performance. Furthermore, the use of histological assays as a valid tool for fish welfare assessment is also discussed, and insights on nutrient absorptive capacity and responsiveness to pathogens in fish by gut morphological endpoints are provided. Overall, the literature reviewed emphasizes the complex interactions between microorganisms and host fish, shedding light on the beneficial use of prebiotics and probiotics in the aquaculture sector, with the potential to provide directions for future research.

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Single and combined effects of caffeine and salicylic acid on mussel Mytilus galloprovincialis: Changes at histomorphological, molecular and biochemical levels

Cited by 5 publications

References 131 publications

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

Non-Steroidal Anti-Inflammatory Drugs in the Aquatic Environment and Bivalves: The State of the Art

Histomorphological Changes in Fish Gut in Response to Prebiotics and Probiotics Treatment to Improve Their Health Status: A Review

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