Single and combined effects of photodynamic therapy and antibiotics to inactivate Staphylococcus aureus on skin

Branco, Tatiana Moura; Valério, Nádia; Jesus, Vânia Isabel Rodrigues; Dias, Cristina J.; Neves, Maria G. P. M. S.; Faustino, Maria A. F.; Almeida, Adelaide

doi:10.1016/j.pdpdt.2018.01.001

Cited by 47 publications

(29 citation statements)

References 58 publications

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“…The direct comparison of in vitro results with results of the porcine skin clearly show that killing of bacteria on the skin requires a higher photosensitizer concentration and radiant exposure, obviously to overcome some unexplored obstacles, which is partially comparable with other studies . This is a crucial point for future clinical application of PIB against bacteria on skin.…”

Section: Discussionsupporting

confidence: 67%

“…A recent paper likewise showed very promising results in an ex vivo skin model using a porphyrin as photosensitizer. In the study a reduction of about 4 log 10 steps of S. aureus was achieved, and even more than 5 log 10 steps when combining PIB with the antibiotic substance ampicillin …”

Section: Discussionmentioning

confidence: 86%

“…The application of PIB has been investigated predominantly in vitro , elaborately reviewed in Wainwright et al ., less frequently in animal models or ex vivo , and only rarely in clinical studies. Among the few clinical studies, PIB application was studied in dentistry but very rarely in skin …”

mentioning

confidence: 99%

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Photodynamic inactivation of bacteria to decolonize meticillin-resistant Staphylococcus aureus from human skin

et al. 2018

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 86%

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Photodynamic inactivation of bacteria to decolonize meticillin-resistant Staphylococcus aureus from human skin

et al. 2018

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“…A probable explanation is that the singlet oxygen and/or other ROS produced by the PSs which are interacting with the membranes are involved in photoperoxidation, which is responsible for the membrane damage, highlighting the paired selectivity (light and PS cellular localization) [11]. One of the most impressive facts is that aPDT is efficient in multidrug-resistant strains and does not encourage resistance [12]; the repeated photosensitization of surviving cells does not induce the selection of resistant microbial strains and does not modify their sensitivity to antibiotic treatment [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

An Insight into Advanced Approaches for Photosensitizer Optimization in Endodontics—A Critical Review

Diogo

Faustino

Neves

et al. 2019

JFB

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Apical periodontitis is a biofilm-mediated disease; therefore, an antimicrobial approach is essential to cure or prevent its development. In the quest for efficient strategies to achieve this objective, antimicrobial photodynamic therapy (aPDT) has emerged as an alternative to classical endodontic irrigation solutions and antibiotics. The aim of the present critical review is to summarize the available evidence on photosensitizers (PSs) which has been confirmed in numerous studies from diverse areas combined with several antimicrobial strategies, as well as emerging options in order to optimize their properties and effects that might be translational and useful in the near future in basic endodontic research. Published data notably support the need for continuing the search for an ideal endodontic photosensitizer, that is, one which acts as an excellent antimicrobial agent without causing toxicity to the human host cells or presenting the risk of tooth discoloration. The current literature on experimental studies mainly relies on assessment of mixed disinfection protocols, combining approaches which are already available with aPDT as an adjunct therapy. In this review, several approaches concerning aPDT efficiency are appraised, such as the use of bacteriophages, biopolymers, drug and light delivery systems, efflux pump inhibitors, negative pressure systems, and peptides. The authors also analyzed their combination with other approaches for aPDT improvement, such as sonodynamic therapy. All of the aforementioned techniques have already been tested, and we highlight the biological challenges of each formulation, predicting that the collected information may encourage the development of other effective photoactive materials, in addition to being useful in endodontic basic research. Moreover, special attention is dedicated to studies on detailed conditions, aPDT features with a focus on PS enhancer strategies, and the respective final antimicrobial outcomes. From all the mentioned approaches, the two which are most widely discussed and which show the most promising outcomes for endodontic purposes are drug delivery systems (with strong development in nanoparticles) and PS solubilizers.

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“…chloramphenicol) or enhance the efficacy of antibiotics targeting the external structure of bacteria (e.g. ampicillin) [76].…”

Section: Staphylococcus Aureusmentioning

confidence: 99%

Multimodal use of the porphyrin TMPyP: From cancer therapy to antimicrobial applications

García-Sampedro

Tabero

Mahamed

et al. 2019

J. Porphyrins Phthalocyanines

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The cationic porphyrin meso-tetra(4-[Formula: see text]-methylpyridyl)porphine (TMPyP) has a high yield of singlet oxygen generation upon light activation and a strong affinity for DNA. These advantageous properties have turned it into a promising photosensitizer for use in photodynamic therapy (PDT). In this review, we have summarized the current state-of-the-art applications of TMPyP for the treatment of cancer as well as its implementation in antimicrobial PDT. The most relevant studies reporting its pharmacokinetics, subcellular localization, mechanism of action, tissue biodistribution and dosimetry are discussed. Combination strategies using TMPyP-PDT together with other photosensitizers and chemotherapeutic agents to achieve synergistic anti-tumor effects and reduce resistance to therapy are also explored. Finally, we have addressed emerging applications of this porphyrin, including nanoparticle-mediated delivery, controlled drug release, biosensing and G-quadruplex stabilization for tumor growth inhibition. Altogether, this work highlights the great potential and versatility that TMPyP can offer in different fields of biomedicine such us cancer treatment or antimicrobial therapy.

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Single and combined effects of photodynamic therapy and antibiotics to inactivate Staphylococcus aureus on skin

Cited by 47 publications

References 58 publications

Photodynamic inactivation of bacteria to decolonize meticillin-resistant Staphylococcus aureus from human skin

Photodynamic inactivation of bacteria to decolonize meticillin-resistant Staphylococcus aureus from human skin

An Insight into Advanced Approaches for Photosensitizer Optimization in Endodontics—A Critical Review

Multimodal use of the porphyrin TMPyP: From cancer therapy to antimicrobial applications

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