Single amino acid mutation of nectin-1 provides remarkable resistance against lethal pseudorabies virus infection in mice
Yukiko TOMIOKA,
Keiko TAKEDA,
Kinuyo OZAKI
et al.
Abstract:An approach to genetically engineered resistance to pseudorabies virus (PRV) infection was examined by using a mouse model with defined point mutation in primary receptor for
alphaherpesviruses, nectin-1, by the CRISPR/Cas9 system. It has become clear that phenylalanine at position 129 of nectin-1 is important for binding to viral glycoprotein D (gD), and
mutation of phenylalanine 129 to alanine (F129A) prevents nectin-1 binding to gD and virus entry
in vitro
. Here, to assess the antivi… Show more
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