2016
DOI: 10.1021/acs.biochem.5b01044
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Single-Amino Acid Modifications Reveal Additional Controls on the Proton Pathway of [FeFe]-Hydrogenase

Abstract: The proton pathway of [FeFe]-hydrogenase is essential for enzymatic H2 production and oxidation and is composed of four residues and a water molecule. A computational analysis of this pathway in the [FeFe]-hydrogenase from Clostridium pasteurianum revealed that the solvent-exposed residue of the pathway (Glu282) forms hydrogen bonds to two residues outside of the pathway (Arg286 and Ser320), implying that these residues could function in regulating proton transfer. In this study, we show that substituting Arg2… Show more

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Cited by 28 publications
(18 citation statements)
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References 42 publications
(104 reference statements)
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“…Potentially involved amino acids have been identified due to phylogenetic conservation 82 and MD simulations. [18][19][20][21] However, cysteine 169 of the proton relay group at the cofactor (Fig. 2) is the only residue that has been verified to propel proton transfer to and from the H-cluster.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Potentially involved amino acids have been identified due to phylogenetic conservation 82 and MD simulations. [18][19][20][21] However, cysteine 169 of the proton relay group at the cofactor (Fig. 2) is the only residue that has been verified to propel proton transfer to and from the H-cluster.…”
Section: Discussionmentioning
confidence: 99%
“…Molecular dynamic (MD) simulations were employed to characterize this conserved proton transfer path. [18][19][20][21] It is formed by two glutamic acid side chains, at least one serine, one or two water molecules, and a cysteine in close vicinity to the adt group of the H-cluster (Fig. 2).…”
Section: This Geometry Stabilizes the Open Coordination Site At Fe D mentioning
confidence: 99%
“…[18][19][20]35 Theoretical studies support a rapid exchange of protons via this 'catalytic' pathway. [53][54][55] However, the interplay between proton transfer and electron transfer events in the hydrogen conversion reaction of [FeFe]hydrogenases remains largely unclear.…”
Section: Introductionmentioning
confidence: 99%
“…part of the W1/adt proton transfer pathway towards Fed. [8][9][10] Cysteine 417 at the cluster is a potential protonation site as part of proton transfer pathway W2.…”
Section: Introductionmentioning
confidence: 99%