Single amino acid exchanges in the finger domain impair the function of the Drosophila gene Krüppel (Kr).

Gaul, Ulrike; Redemann, Norbert; Jäckle, Herbert

doi:10.1073/pnas.86.12.4599

Cited by 12 publications

(4 citation statements)

References 21 publications

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“…1H). This finding is in agreement with a weak Kr mutant segmentation defect gen- erated by such a replacement (39), and it argues that Kr function is not as strongly impaired as in the first case. The molecular analyses of two of several EMS-induced Kr revertants establish unequivocally allelism between If and Kr.…”

Section: R2supporting

confidence: 80%

A modifier screen in the eye reveals control genes for Krüppel activity in the Drosophila embryo

Carrera

Abrell

Kerber

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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Irregular facets (If ) is a dominant mutation of Drosophila that results in small eyes with fused ommatidia. Previous results showed that the gene Krüppel (Kr), which is best known for its early segmentation function, is expressed ectopically in If mutant eye discs. However, it was not known whether ectopic Kr activity is either the cause or the result of the If mutation. Here, we show that If is a gain-of-function allele of Kr. We then used the If mutation in a genetic screen to identify dominant enhancers and suppressors of Kr activity on the third chromosome. Of 30 identified Kr-interacting loci, two were cloned, and we examined whether they also represent components of a natural Kr-dependent developmental pathway of the embryo. We show that the two genes, eyelid (eld) and extramacrochaetae (emc), which encode a Bright family-type DNA binding protein and a helix-loop-helix factor, respectively, are necessary to achieve the singling-out of a unique Kr-expressing cell during the development of the Malpighian tubules, the excretory organs of the f ly. The results indicate that the Kr gain-of-function mutation If provides a tool to identify genes that are active during eye development and that a number of them function also in the control of Kr-dependent developmental processes.

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Section: R2supporting

confidence: 80%

A modifier screen in the eye reveals control genes for Krüppel activity in the Drosophila embryo

Carrera

Abrell

Kerber

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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“…To determine if both, or just the C64 transrepressor domains of Kru Èppel are required to provide h7-mediated repression in vivo, we made use of a previously identi®ed mutant Kru Èppel allele, Kr v (Gaul et al, 1989). Kr v is a stop codon mutation that terminates Kru Èppel in position 351 and consequently, encodes Kr proteins that lack the C64 domain.…”

Section: The C-terminal Region Of Kru èPpel Is Essential For Repressimentioning

confidence: 99%

hairy stripe 7 element mediates activation and repression in response to different domains and levels of Krüppel in the Drosophila embryo

Rosée-Borggreve

Häder

Wainwright

et al. 1999

Mechanisms of Development

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The Drosophila gap gene Krüppel (Kr) encodes a zinc finger-type transcription factor required for controlling the spatial expression of other segmentation genes during early blastoderm stage. Here we show that two independent and transferable repressor domains of Krüppel act to control expression of the pair-rule gene hairy, and that the minimal cis-acting element of hairy stripe7 (h7) mediates either Krüppel-dependent activation or repression in different regions of the blastoderm embryo. The C-terminal region of Krüppel which encompasses the predominant repressor domain is not essential for activation, but is required to fully suppress h7-mediated transcription in response to high levels of Krüppel activity. This domain contains an interaction motif for dCtBP, a homologue of the human co-repressor CtBP. dCtBP activity is, however, dispensable for Krüppel-mediated repression in the embryo since Krüppel-mediated repression functions in the absence of dCtBP. Possible modes of h7-mediated gene regulation in response to the different domains and levels of Krüppel are discussed.

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“…Genetic evidence suggests that fkh , in turn, is required for the activation of Kr at the posterior pole of the blastoderm embryo and later in a ring of MT primordial cells at the hindgut/midgut boundary of the developing alimentary canal. In the absence of Kr activity, the MT precursor cells become integrated into the hindgut epithelium and no MTs arise (Gloor, 1950; Gaul et al ., 1989; Harbecke and Janning, 1989; Gaul and Weigel, 1991). During tubule eversion, Kr expression becomes gradually restricted to a single cell, the ‘tip mother cell’, in each of the four tubule precursors (Hoch et al ., 1994).…”

Section: Introductionmentioning

confidence: 99%

Kruppel acts as a developmental switch gene that mediates Notch signalling-dependent tip cell differentiation in the excretory organs of Drosophila

Hoch

Jäckle

1998

The EMBO Journal

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Single amino acid exchanges in the finger domain impair the function of the Drosophila gene Krüppel (Kr).

Cited by 12 publications

References 21 publications

A modifier screen in the eye reveals control genes for Krüppel activity in the Drosophila embryo

A modifier screen in the eye reveals control genes for Krüppel activity in the Drosophila embryo

hairy stripe 7 element mediates activation and repression in response to different domains and levels of Krüppel in the Drosophila embryo

Kruppel acts as a developmental switch gene that mediates Notch signalling-dependent tip cell differentiation in the excretory organs of Drosophila

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