SINEVA polymorphism and mapping of the bovine pregnancy-associated glycoprotein 1 gene

Martín-Burriel, Inmaculada; Elduque, C.; Osta, Rosario; Laurent, Pascal; Barendse, W.; Zaragoza, Pilar

doi:10.1007/s003359900720

Cited by 2 publications

(1 citation statement)

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“…The gene product is produced specifically in the invasive binucleate cells of the placenta and becomes detectable in maternal serum soon after implantation (Xie at al., 1995). PAG1B was just recently placed on BTA29 between anonymous markers RM40 and ILSTS081 using SINEVA (Sine variable poly(A)) markers (Martin-Burriel et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

High-resolution genetic map of bovine chromosome 29 through focused marker development

Reed¹,

Ihara²,

Mariani³

et al. 2002

Cytogenet Genome Res

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Chromosome-specific libraries aid in the development of genetic maps and focus marker development in areas of the genome with identified quantitative trait loci (QTL). A small-insert BTA29 library constructed by microdissection of a 1:29 Rb-fusion cell line, was screened for dinucleotide repeats (CA)₁₅ and/or (GA)₁₅ with the goal of generating new genetic markers for this, the smallest bovine autosome. A total of 90 primer pairs were designed and 82 of these successfully amplified bovine genomic DNA by PCR. In addition to these 82 loci, primer pairs were developed for nine putative genes identified from the sequenced clones by BLAST searches of GenBank. A somatic cell panel was used to test for synteny of the new loci with two previously mapped BTA29 markers located on the MARC bovine linkage map. A total of 75 of the 82 microsatellite (ms) loci were integrated into the MARC bovine linkage map. Linkage analysis placed 69 ms markers on BTA29, five on BTAX and one on BTA1. Combined results of the somatic cell and linkage analyses place 79 new markers (ms and gene-related) on BTA29, six loci on BTAX and two loci on BTA1. The results of this effort significantly increase the marker density on BTA29, expanding the ability to fine map QTL associated with this chromosome.

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Section: Discussionmentioning

confidence: 99%