SINEUPs: A new class of natural and synthetic antisense long non-coding RNAs that activate translation

Zucchelli, S.; Cotella, Diego; Takahashi, Hazuki; Carrieri, Claudia; Cimatti, Laura; Fasolo, Francesca; Jones, Michael H.; Sblattero, Daniele; Sanges, Remo; Santoro, Claudio; Persichetti, Francesca; Carninci, Piero; Gustincich, Stefano

doi:10.1080/15476286.2015.1060395

Cited by 72 publications

(98 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, we found that uc010rul decreased the protein level of ARHGAP42 but not the mRNA level of ARHGAP42, thus indicating that uc010rul may inhibit ARHGAP42 at a post‐transcriptional level. Natural antisense lncRNAs regulate sense gene expression through different mechanisms, such as transcription collision, DNA methylation modification, RNA splicing, and mRNA translation 28, 29, 30. The most common pattern of natural antisense lncRNA regulation on sense genes may be inhibition of the sense gene translation process through specific secondary structure.…”

Section: Discussionmentioning

confidence: 99%

ARHGAP42 promotes cell migration and invasion involving PI3K/Akt signaling pathway in nasopharyngeal carcinoma

Lin

Ding

et al. 2018

Cancer Medicine

View full text Add to dashboard Cite

Rho GTPase‐activating protein 42 was identified as an inhibitor of RhoA to maintain normal blood pressure homeostasis. However, the effect of ARHGAP42 in promoting cell malignancy in nasopharyngeal carcinoma is demonstrated in this study. Microarray and real‐time quantitative PCR were used for a mRNA profiling of ARHGAP42 in nasopharyngeal primary and metastatic carcinoma tissues. Western blot and immunohistochemical staining were used for detecting the expression of ARHGAP42 protein in nasopharyngeal carcinoma tissues and cell lines. The overexpression and silence experiments of ARHGAP42 were performed in NPC cell lines using siRNA and expressive plasmid for evaluating cancer cell migration and invasion in vitro. Real‐time quantitative PCR, western blot, and transwell test were employed for with the function of ARHGAP42 and its antisense lncRNA uc010rul. We confirmed the elevated expression of ARHGAP42 in metastatic NPC tissues of mRNA and protein for the first time. Immunohistochemical analysis indicated that NPC patients with highly ARHGAP42 expression were significantly associated with shorter metastasis‐free survival. Knockdown of ARHGAP42 resulted in significant inhibition of nasopharyngeal cancer cell migration and invasion in vitro, and the overexpression of ARHGAP42 showed the opposite effects. In addition, the silence of uc010rul resulted in ARHGAP42 expression decrease and significant inhibition of nasopharyngeal cancer cell migration and invasion. High expression of ARHGAP42 is associated with poor metastasis‐free survival of nasopharyngeal carcinoma patients. ARHGAP42 promotes migration and invasion of nasopharyngeal carcinoma cells in vitro; the antisense lncRNA may be involved in this effect.

show abstract

Section: Discussionmentioning

confidence: 99%

ARHGAP42 promotes cell migration and invasion involving PI3K/Akt signaling pathway in nasopharyngeal carcinoma

Lin

Ding

et al. 2018

Cancer Medicine

View full text Add to dashboard Cite

show abstract

“…These antisense lncRNAs perform many biological functions and regulate the corresponding sense mRNAs at the transcriptional or post‐transcriptional level . Antisense lncRNAs induce changes in the epigenetic inheritance of chromatins and DNAs and thereby affect the expression of the corresponding sense mRNAs . To determine the functions of lncRNAs, we integrated the antisense lncRNAs and corresponding sense mRNAs that were differentially expressed between AGA and normal tissues.…”

Section: Resultsmentioning

confidence: 99%

Genomewide differential expression profiling of long non‐coding RNAs in androgenetic alopecia in a Chinese male population

Bao

Ying

et al. 2017

Acad Dermatol Venereol

View full text Add to dashboard Cite

show abstract

“…As such, BD can be designed to redirect translation up-regulation activity to potentially any target gene of interest. Gene-specific BDs are typically designed around the initiating AUG codon and overlapping part of the 5′ untranslated sequence and a portion of the coding sequence [98]. Despite the exact rules governing sense mRNA and SINEUP interaction are presently not known, increasing number of examples suggest a certain degree of flexibility in BD design (unpublished data).…”

Section: Focus On the Enhancement Of Translationmentioning

confidence: 99%

Engineering Translation in Mammalian Cell Factories to Increase Protein Yield: The Unexpected Use of Long Non-Coding SINEUP RNAs

Zucchelli

Patrucco

Persichetti

et al. 2016

Computational and Structural Biotechnology Journal

View full text Add to dashboard Cite

Mammalian cells are an indispensable tool for the production of recombinant proteins in contexts where function depends on post-translational modifications. Among them, Chinese Hamster Ovary (CHO) cells are the primary factories for the production of therapeutic proteins, including monoclonal antibodies (MAbs). To improve expression and stability, several methodologies have been adopted, including methods based on media formulation, selective pressure and cell- or vector engineering. This review presents current approaches aimed at improving mammalian cell factories that are based on the enhancement of translation. Among well-established techniques (codon optimization and improvement of mRNA secondary structure), we describe SINEUPs, a family of antisense long non-coding RNAs that are able to increase translation of partially overlapping protein-coding mRNAs. By exploiting their modular structure, SINEUP molecules can be designed to target virtually any mRNA of interest, and thus to increase the production of secreted proteins. Thus, synthetic SINEUPs represent a new versatile tool to improve the production of secreted proteins in biomanufacturing processes.

show abstract

SINEUPs: A new class of natural and synthetic antisense long non-coding RNAs that activate translation

Cited by 72 publications

References 46 publications

ARHGAP42 promotes cell migration and invasion involving PI3K/Akt signaling pathway in nasopharyngeal carcinoma

ARHGAP42 promotes cell migration and invasion involving PI3K/Akt signaling pathway in nasopharyngeal carcinoma

Genomewide differential expression profiling of long non‐coding RNAs in androgenetic alopecia in a Chinese male population

Engineering Translation in Mammalian Cell Factories to Increase Protein Yield: The Unexpected Use of Long Non-Coding SINEUP RNAs

Contact Info

Product

Resources

About