Sin3 is involved in cell size control at Start in <i>Saccharomyces cerevisiae</i>

Stephan, Octavian O H; Koch, Christian A.

doi:10.1111/j.1742-4658.2009.07095.x

Cited by 6 publications

(5 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Having verified the interaction of dKDM5/LID with SIN3 and the catalytic component of the complex, the HDAC RPD3, we sought to determine the contribution of dKDM5/LID to SIN3 complex functions. Previous work in yeast, flies, and mammals has shown SIN3 to play an important role in regulating progression through the cell cycle [ 9 , 11 , 17 , 46 ]. In Drosophila, in addition to SIN3, other components of the SIN3 complex such as RPD3 and p55 have also been shown to affect cell growth rates of cultured cells [ 17 ].…”

Section: Resultsmentioning

confidence: 99%

The histone demethylase dKDM5/LID interacts with the SIN3 histone deacetylase complex and shares functional similarities with SIN3

Gajan

Barnes

Liu

et al. 2016

Epigenetics & Chromatin

View full text Add to dashboard Cite

BackgroundRegulation of gene expression by histone-modifying enzymes is essential to control cell fate decisions and developmental processes. Two histone-modifying enzymes, RPD3, a deacetylase, and dKDM5/LID, a demethylase, are present in a single complex, coordinated through the SIN3 scaffold protein. While the SIN3 complex has been demonstrated to have functional histone deacetylase activity, the role of the demethylase dKDM5/LID as part of the complex has not been investigated.ResultsHere, we analyzed the developmental and transcriptional activities of dKDM5/LID in relation to SIN3. Knockdown of either Sin3A or lid resulted in decreased cell proliferation in S2 cells and wing imaginal discs. Conditional knockdown of either Sin3A or lid resulted in flies that displayed wing developmental defects. Interestingly, overexpression of dKDM5/LID rescued the wing developmental defect due to reduced levels of SIN3 in female flies, indicating a major role for dKDM5/LID in cooperation with SIN3 during development. Together, these observed phenotypes strongly suggest that dKDM5/LID as part of the SIN3 complex can impact previously uncharacterized transcriptional networks. Transcriptome analysis revealed that SIN3 and dKDM5/LID regulate many common genes. While several genes implicated in cell cycle and wing developmental pathways were affected upon altering the level of these chromatin factors, a significant affect was also observed on genes required to mount an effective stress response. Further, under conditions of induced oxidative stress, reduction of SIN3 and/or dKDM5/LID altered the expression of a greater number of genes involved in cell cycle-related processes relative to normal conditions. This highlights an important role for SIN3 and dKDM5/LID proteins to maintain proper progression through the cell cycle in environments of cellular stress. Further, we find that target genes are bound by both SIN3 and dKDM5/LID, however, histone acetylation, not methylation, plays a predominant role in gene regulation by the SIN3 complex.ConclusionsWe have provided genetic evidence to demonstrate functional cooperation between the histone demethylase dKDM5/LID and SIN3. Biochemical and transcriptome data further support functional links between these proteins. Together, the data provide a solid framework for analyzing the gene regulatory pathways through which SIN3 and dKDM5/LID control diverse biological processes in the organism.Electronic supplementary materialThe online version of this article (doi:10.1186/s13072-016-0053-9) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultsmentioning

confidence: 99%

The histone demethylase dKDM5/LID interacts with the SIN3 histone deacetylase complex and shares functional similarities with SIN3

Gajan

Barnes

Liu

et al. 2016

Epigenetics & Chromatin

View full text Add to dashboard Cite

show abstract

“…Several studies have indicated a good correlation between SIN3 and HDAC direct target genes. Stephan and Koch (2009) found that Sin3 and HDAC Rpd3 were both recruited to promoters of SBF (Swi4/Swi6)-regulated genes (CLN1, CLN2, and PCL1) in yeast. van Oevelen et al (2008van Oevelen et al ( , 2010 showed by ChIP assay that SIN3 directly impacts acetylation levels downstream of the transcriptional start site of target genes in mouse.…”

Section: Snl1 and Snl2 Regulate Gene Transcription Through Histone Dementioning

confidence: 99%

ArabidopsisPaired Amphipathic Helix Proteins SNL1 and SNL2 Redundantly Regulate Primary Seed Dormancy via Abscisic Acid–Ethylene Antagonism Mediated by Histone Deacetylation

et al. 2013

View full text Add to dashboard Cite

Histone (de)acetylation is a highly conserved chromatin modification that is vital for development and growth. In this study, we identified a role in seed dormancy for two members of the histone deacetylation complex in Arabidopsis thaliana, SIN3-LIKE1 (SNL1) and SNL2. The double mutant snl1 snl2 shows reduced dormancy and hypersensitivity to the histone deacetylase inhibitors trichostatin A and diallyl disulfide compared with the wild type. SNL1 interacts with HISTONE DEACETYLASE19 in vitro and in planta, and loss-of-function mutants of SNL1 and SNL2 show increased acetylation levels of histone 3 lysine 9/18 (H3K9/18) and H3K14. Moreover, SNL1 and SNL2 regulate key genes involved in the ethylene and abscisic acid (ABA) pathways by decreasing their histone acetylation levels. Taken together, we showed that SNL1 and SNL2 regulate seed dormancy by mediating the ABA-ethylene antagonism in Arabidopsis. SNL1 and SNL2 could represent a crosslink point of the ABA and ethylene pathways in the regulation of seed dormancy.

show abstract

“…Furthermore, mSin3 has been identified as a critical component in the permanent repression of cell cycle genes during differentiation [26]. Although not essential for yeast viability in normal conditions, ySin3 was shown to be important for accurate timing of Swi4/Swi6-regulated gene transcription during G1 and for activation of S phase in daughter cells [27]. Additionally, S. cerevisiae SIN3 was found to be important for cell cycle progression as null mutants of an asynchronous population accumulated in G2 phase relative to wild type cells [28].…”

Section: Introductionmentioning

confidence: 99%

SIN3 is critical for stress resistance and modulates adult lifespan

Barnes

Bhat

Unnikrishnan

et al. 2014

Aging

View full text Add to dashboard Cite

Coordinate control of gene activity is critical for fitness and longevity of an organism. The SIN3 histone deacetylase (HDAC) complex functions as a transcriptional repressor of many genes. SIN3-regulated genes include those that encode proteins affecting multiple aspects of mitochondrial function, such as energy production and stress responsiveness, important for health maintenance. Here we used Drosophila melanogaster as a model organism to examine the role of SIN3 in the regulation of fitness and longevity. Adult flies with RNA interference (RNAi) induced knockdown expression of Sin3A have reduced climbing ability; an activity that likely requires fully functional mitochondria. Additionally, compared to wild type, adult Sin3A knockdown flies were more sensitive to oxidative stress. Interestingly, media supplementation with the antioxidant glutathione largely restored fly tolerance to oxidative stress. Although Sin3A knockdown flies exhibited decreased longevity compared to wild type, no significant changes in expression of many well-categorized aging genes were observed. We found, however, that Sin3A knockdown corresponded to a significant reduction in expression of genes encoding proteins involved in the de novo synthesis of glutathione. Taken together, the data support a model whereby SIN3 regulates a gene expression program required for proper mitochondrial function and effective stress response during adulthood.

show abstract

Sin3 is involved in cell size control at Start in Saccharomyces cerevisiae

Cited by 6 publications

References 61 publications

The histone demethylase dKDM5/LID interacts with the SIN3 histone deacetylase complex and shares functional similarities with SIN3

The histone demethylase dKDM5/LID interacts with the SIN3 histone deacetylase complex and shares functional similarities with SIN3

ArabidopsisPaired Amphipathic Helix Proteins SNL1 and SNL2 Redundantly Regulate Primary Seed Dormancy via Abscisic Acid–Ethylene Antagonism Mediated by Histone Deacetylation

SIN3 is critical for stress resistance and modulates adult lifespan

Contact Info

Product

Resources

About