Simvastatin treatment inhibits hypoxia inducible factor 1-alpha-(HIF-1alpha)-prolyl-4-hydroxylase 3 (PHD-3) and increases angiogenesis after myocardial infarction in streptozotocin-induced diabetic rat

Deletion of prolyl hydroxylase domain proteins (PHD1, PHD3) stabilizes hypoxia inducible factor-1 alpha, promotes neovascularization, and improves perfusion in a murine model of hind-limb ischemia

“…VEGF quantification was performed via ELISA as previously described (Thirunavukkarasu et al, 2013). Briefly, mice from all three groups (WT, PHD1 −/− and PHD3 −/− mice) were euthanized 3 days after femoral artery ligation and ischemic hind limb gastrocnemius muscle tissue sections were harvested from each treatment group.…”

“…Ischemic limb muscles were collected 3 days after HLI and tissue sections were prepared as described previously and used for immunofluorescence staining (Thirunavukkarasu et al, 2013;Limbourg et al, 2009). Briefly, the sections were stained for Bcl-2 and HIF1-α with rabbit polyclonal anti-Bcl-2 and rabbit polyclonal anti-HIF-1α (Santa Cruz Biotechnology, CA, USA), respectively, and incubated overnight at 4°C.…”

Influence of Statin Pretreatment on Initial Neurological Severity and Short-Term Functional Outcome in Acute Ischemic Stroke Patients: The Fukuoka Stroke Registry

“…Diabetic patients show diminished cerebral perfusion, and an experimental stroke model revealed impaired collateral flow in diabetic mice [27,28]. In addition, although the effect of statin treatment on cerebral collateral flow according to the presence of diabetes mellitus has not been determined, an experimental study showed that statin enhances coronary collateralization more prominently in diabetic than in non-diabetic rats [29]. Pre-stroke statin treatment also appeared to show a more favorable effect on initial neurological severity in patients with first-ever stroke than in those with recurrent stroke.…”

Thioredoxin-1 (Trx1) engineered mesenchymal stem cell therapy increased pro-angiogenic factors, reduced fibrosis and improved heart function in the infarcted rat myocardium

“…To analyze the effect of Trx1-engineered MSC therapy on vasculogenesis, immunohistochemical analysis of caveolin-1, a marker for endothelial cells, was performed as described earlier [31][32][33][34].…”

“…The tissue was fixed, and paraffinembedded sections were made and stained with picrosirius red as described earlier [34]. Five to 10 images per section were digitally acquired at 400× magnification using an Olympus QColor 3TM digital camera mounted on an Olympus BH2 microscope.…”