Simultaneously and separately immobilizing incompatible dual-enzymes on polymer substrate via visible light induced graft polymerization

Zhu, Xing; He, Bin; Zhao, Changwen; Ma, Yuhong; Yang, Wantai

doi:10.1016/j.apsusc.2017.11.284

Cited by 15 publications

(3 citation statements)

References 47 publications

(50 reference statements)

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“…In recent years, immobilized enzyme reactors (IMERs) have attracted considerable attention as a means of stabilizing enzymes. Another notable feature of IMERs is that they can be combined with other separation and detection techniques such as CE, HPLC, and MS . Of these techniques, CE has great potential because it is characterized by rapid analysis, diverse separation modes, high separation efficiency, and low sample consumption, and it is applicable to biomacromolecule assays.…”

Section: Introductionmentioning

confidence: 99%

Controllable and high‐performance immobilized enzyme reactor: DNA‐directed immobilization of multienzyme in polyamidoamine dendrimer‐functionalized capillaries

Shen

Zhou

et al. 2020

Electrophoresis

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In recent years, CE‐integrated immobilized enzyme reactors (IMERs) for single‐enzyme immobilization have attracted considerable attention. However, there has been little research on multienzyme immobilization in CE. Here, we introduce a method for fabricating a CE‐integrated IMER, using DNA‐directed immobilization to fix glucose oxidase and horseradish peroxidase in the capillary, which had been functionalized with polyamidoamine dendrimer (PAMAM). Owing to the reversibility of DNA hybridization, the reactor is capable of dynamic immobilization. Moreover, by introducing the PAMAM, the loading capacity of the IMER is greatly enhanced, and the PAMAM can spontaneously form complexes with DNA and then contribute to the efficiency and stability of the reactor. After 25 days storage, the prepared IMER ultimately retained approximately 70% of its initial activity. We also used the IMER to detect glucose, and the favorable linearity was obtained over the concentration range of 0.78–12.5 mM, with an LOD of 0.39 mM, demonstrating that the CE‐integrated IMER can be applied to actual samples. We believe that this strategy can be extended to other multienzyme immobilization systems, and CE‐integrated IMERs are potentially useful in a wide range of biochemical research applications.

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Section: Introductionmentioning

confidence: 99%

Controllable and high‐performance immobilized enzyme reactor: DNA‐directed immobilization of multienzyme in polyamidoamine dendrimer‐functionalized capillaries

Shen

Zhou

et al. 2020

Electrophoresis

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“…Lipase has been immobilized onto different polymeric films, such as polypropylene and the process have been assessed by spectroscopic analysis . Polymeric films in a microchip are useful for high‐density enzyme immobilization . Polymer‐based materials, including polydimethylsiloxane, poly(methyl methacrylate), polycarbonate and Teflon, have been extensively used to fabricate microchips using photolithography, soft lithography and micromachining using lasers or microdrilling .…”

Section: Introductionmentioning

confidence: 99%

“…10 Polymeric films in a microchip are useful for high-density enzyme immobilization. 11 Polymer-based materials, including polydimethylsiloxane, poly(methyl methacrylate), polycarbonate and Teflon, have been extensively used to fabricate microchips using photolithography, soft lithography and micromachining using lasers or microdrilling. 12 Photopatterning enables the modification of channel surfaces with a high degree of spatial precision.…”

Section: Introductionmentioning

confidence: 99%

Enzyme immobilization on photopatterned temperature‐response poly (N‐isopropylacrylamide) for microfluidic biocatalysis

Zhu

Xiong

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND Microfluidic chips have gained growing attention from the scientific community due to their large surface/interface area and fast mass transfer. However, their application is challenged by the instability of enzymes and time‐consuming catalytic process. Poly (N‐isopropylacrylamide) (PNIPAAm) coupled with photopatterning technology was applied to immobilize an enzyme on the microchannel surface to realize the biotransformation in this study. RESULTS The photopattern‐immobilized naringinase on a microchip achieved a yield of 93.63 ± 1.12% for isoquercitrin production within 5 min. The production of unit time per unit enzyme (g g−1 h−1) increased 2.1‐fold whereas the reaction time decreased to 1/12 of the time required in a batch reactor. The enzyme was absorbed and desorbed at 40 and 25 °C, respectively, and the release efficiency of immobilized naringinase reached 80.57%, indicating that most of the enzymes were replaced with fresh enzymes to proceed each enzymatic reaction. Six cycles of enzymatic hydrolysis reactions were completed successively, maintaining >60% of relative enzymatic activity. CONCLUSION The results indicated that using the immobilized enzyme on the photopatterned PNIPAAm in the enzymatic hydrolysis of rutin was an efficient and simple way to achieve a high yield of isoquercitrin. Thus, this approach represents a convenient and cost‐saving method to produce fine chemicals using microfluidic biocatalysis. © 2019 Society of Chemical Industry

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Nanomaterials for co‐immobilization of multiple enzymes

Zhang,

Lovell,

Shi

et al. 2024

BMEMat

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In order to co‐immobilize multiple enzymes, a wide range of nanomaterials has been designed to achieve synergistic enzyme activity and enhance catalytic efficiency. Nanomaterials, as carriers for enzyme co‐immobilization, possess various advantages such as tunable morphology and size, high specific surface area, and abundant chemically active sites. They can significantly enhance enzyme stability, activity, and catalytic efficiency. We overview the commonly used methods and strategies of enzyme co‐immobilization. This review further summarizes the latest research advances in nanomaterials for enzyme co‐immobilization applications over the past 5 years. Meanwhile, the advantages and challenges of these nanomaterials used for enzyme co‐immobilization as well as some potential future directions are also discussed.

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Simultaneously and separately immobilizing incompatible dual-enzymes on polymer substrate via visible light induced graft polymerization

Cited by 15 publications

References 47 publications

Controllable and high‐performance immobilized enzyme reactor: DNA‐directed immobilization of multienzyme in polyamidoamine dendrimer‐functionalized capillaries

Controllable and high‐performance immobilized enzyme reactor: DNA‐directed immobilization of multienzyme in polyamidoamine dendrimer‐functionalized capillaries

Enzyme immobilization on photopatterned temperature‐response poly (N‐isopropylacrylamide) for microfluidic biocatalysis

Nanomaterials for co‐immobilization of multiple enzymes

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