Simultaneous wastewater treatment and lipid production by Scenedesmus sp. HXY2

Ye, Sisi; Gao, Li; Zhao, Jing; An, Mei; Wu, Haiming; Li, Ming

doi:10.1016/j.biortech.2020.122903

Cited by 71 publications

(28 citation statements)

References 39 publications

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“…This difference might be related to the relatively stable microalgal growth in 20× and 30× cultures in that fatty acid compositions in 20× and 30× cultures were more similar to those in axenic cultures of Scenedesmus spp. [26,32] than were the compositions in 5× and 10× cultures. The quality of biodiesel mainly depends on fatty acid composition in triacylglycerol.…”

Section: Lipid and Fames Profilementioning

confidence: 77%

Evaluation of Scenedesmus rubescens for Lipid Production from Swine Wastewater Blended with Municipal Wastewater

2020

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This study examined the feasibility of using non-sterilized swine wastewater for lipid production by an isolated microalga, Scenedesmus rubescens. Different dilution ratios using municipal wastewater as a diluent were tested to determine the suitable levels of microalgal growth in the wastewaters, its nutrient removal, and its lipid production. The highest lipid productivity (8.37 mg/L/d) and NH4+ removal (76.49%) were achieved in swine wastewater that had been diluted to 30 times using municipal wastewater. Various bacteria coexisted in the wastewaters during the cultivation of S. rubescens. These results suggest the practical feasibility of a system to produce lipids from swine wastewater by using microalgae.

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Section: Lipid and Fames Profilementioning

confidence: 77%

Evaluation of Scenedesmus rubescens for Lipid Production from Swine Wastewater Blended with Municipal Wastewater

2020

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“…Due to the potential applications of Scenedesmus strains in wastewater treatment and lipid production [31,32], their lipid metabolism needs to be well-studied. S. obliquus AS-6-11 has fewer genes of lipid metabolism compared to S. quadricauda LWG002611 and the other four green microalgae, suggesting the difference of lipid biosynthesis or genome integrity among the strains.…”

Section: Discussionmentioning

confidence: 99%

Genome sequencing, assembly, and annotation of the self-flocculating microalga Scenedesmus obliquus AS-6-11

Chen

Mhuantong²,

et al. 2020

Preprint

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Background Scenedesmus obliquus belongs to green microalgae and is widely used in aquaculture as feed, which is also explored for lipid production and bioremediation. However, genomic studies of this microalga have been very limited. Cell self-flocculation of microalgal cells can be used as a simple and economic method for harvesting biomass, and it is of great importance to perform genome-scale studies for the self-flocculating S. obliquus strains to promote their biotechnological applications. Results We sequenced the genome of the self-flocculating microalga S. obliquus AS-6-11 employing the Pacific Biosciences sequencing platform, and used the MECAT software for de novo genome assembly. The estimated genome size of S. obliquus AS-6-11 is 172.3 Mbp with an N50 of 94,410 bp, and 31,964 protein-coding genes were identified. Gene Ontology (GO) and KEGG pathway analysis revealed 65 GO terms and 428 biosynthetic pathways. Comparing to the well-studied green microalgae Chlamydomonas reinhardtii, Chlorella variabilis, Volvox carteri and Micractinium conductrix, the genome of S. obliquus AS-6-11 encodes more unique proteins, and some of them might be responsible for the self-flocculating phenotype. These genes encode D-mannose binding lectin, glycosylphosphatidylinositol (GPI)-anchored cell wall proteins, and proteins with fasciclin domains that are commonly found in cell surface proteins, respectively. Four genes encoding both GPI-anchored cell wall proteins and fasciclin domain proteins are the most interesting targets for further studies. Conclusions To our best knowledge, this is the first report on the in-depth annotation of the S. obliquus genome, and the results will facilitate functional genomic studies of this important microalga. The comparative genomic analysis here also provides new insights into the evolution of green microalgae. Furthermore, identification of the potential genes encoding proteins for the self-flocculation of microalgal cells will benefit studies on the molecular mechanism underlying this phenotype for its better control and biotechnological applications as well.

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“…More recent studies have shown that Scenedesmus sp. had a high removal of TN, TP, NH 4 + -N (>95%) in simulated wastewater, with a biomass production of 7.2 × 10 6 cells mL −1 and lipid productivity of 5.67 mg L −1 d −1 during 12-day cultivation [11]. In addition, a previous study has shown that C. vulgaris can be cultured in undiluted anaerobically treated swine wastewater in an open raceway pond, with a TN removal of 89.5% and TP removal of 85.3%, respectively [9].…”

Section: Introductionmentioning

confidence: 99%

“…Besides, they have greater photosynthetic efficiency that is 10~50 times higher than terrestrial plants [10]. Reports have shown that many microalgae are exceedingly rich in oil and their lipid contents ranged from 47.9~72% [11]. The lipid productivity of microalgae is 15~300 times that of conventional crops [12].…”

Section: Introductionmentioning

confidence: 99%

Exploration of Microalgal Species for Nutrient Removal from Anaerobically Digested Swine Wastewater and Potential Lipids Production

et al. 2021

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Bio-treatment of anaerobically treated swine wastewater (ADSW) mediated by microalgae has been deemed as a promising strategy. In the present study, six microalgal strains were used to conduct batch experiments in 0~100% ADSW in order to evaluate their potentials for nutrient removal and biodiesel production. Two strains, Chlorella vulgaris FACHB-8 and Chlorella sp. FACHB-31, were selected based on their better growth performances, higher tolerance to wastewater (up to 100%), and better nutrient removal abilities. The capacity of each strain to remove TN, TP, NH4+-N, as well as lipid production and biomass composition in 100% ADSW were further examined. After 15 days of culture, 87.68~89.85%, 92.61~93.68%, and 97.02~97.86% of the nitrogen, phosphorus, and ammonia nitrogen were removed by Chlorella sp. FACHB-31 and C. vulgaris FACHB-8. Their lipid content and lipid productivities were 29.63~33.33% and 18.91~23.10 mg L−1 d−1, respectively. Proteins were both the major biomass fraction followed by lipids and then carbohydrates. Their fatty acid profiles both mainly consisted of C-16:0, C-18:1, C-18:0, and C-18:2. Taken together, our results suggest that C. vulgaris FACHB-8 and Chlorella sp. FACHB-31 are potential candidates for biodiesel production by using ADSW as a good feedstock.

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Simultaneous wastewater treatment and lipid production by Scenedesmus sp. HXY2

Cited by 71 publications

References 39 publications

Evaluation of Scenedesmus rubescens for Lipid Production from Swine Wastewater Blended with Municipal Wastewater

Evaluation of Scenedesmus rubescens for Lipid Production from Swine Wastewater Blended with Municipal Wastewater

Genome sequencing, assembly, and annotation of the self-flocculating microalga Scenedesmus obliquus AS-6-11

Exploration of Microalgal Species for Nutrient Removal from Anaerobically Digested Swine Wastewater and Potential Lipids Production

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