Simultaneous visual detection of multiple heavy metal ions by a high-throughput fluorescent probe

Che, Huachao; Tian, Xike; Chen, Wei; Dai, Chu; Nie, Yulun; Li, Yong; Lu, Liqiang

doi:10.1007/s00604-023-05882-0

Cited by 2 publications

(1 citation statement)

References 40 publications

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“…After standing for 4.5 h, the luminous intensity of each gel bead was measured, and the concentrations of As(III) and As(V) were calculated according to the calibration curves. All gel beads were tested 3 times in parallel, and the average was used as the final result. , As shown in Supporting Information S12, biosensors achieved simultaneous detection of As(III) and As(V) coexisting in actual groundwater samples, and not only were the results consistent with HPLC–ICP–MS but also they do not require any sample preprocessing steps. Therefore, core–shell gel beads can be conveniently used for simultaneously monitoring coexisting As(III) and As(V) in the field.…”

Section: Resultsmentioning

confidence: 99%

A Stable and Sensitive Engineering Bacterial Sensor via Physical Biocontainment and Two-Stage Signal Amplification

Che,

Wang,

et al. 2024

Anal. Chem.

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Although engineering bacterial sensors have outstanding advantages in reflecting the actual bioavailability and continuous monitoring of pollutants, the potential escape risk of engineering microorganisms and lower detection sensitivity have always been one of the biggest challenges limiting their wider application. In this study, a core–shell hydrogel bead with functionalized silica as the core and alginate-polyacrylamide as the shell have been developed not only to realize zero escape of engineered bacteria but also to maintain cell activity in harsh environments, such as extremely acidic/alkaline pH, high salt concentration, and strong pressure. Particularly, after combining the selective preconcentration toward pollutants by functionalized core and the positive feedback signal amplification of engineering bacteria, biosensors have realized two-stage signal amplification, significantly improving the detection sensitivity and reducing the detection limit. In addition, this strategy was actually applied to the detection of As(III) and As(V) coexisting in environmental samples, and the detection sensitivity was increased by 3.23 and 4.39 times compared to sensors without signal amplification strategy, respectively, and the detection limits were as low as 0.39 and 0.86 ppb, respectively.

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Section: Resultsmentioning

confidence: 99%