Simultaneous Two- and Three-Photon Deep Imaging of Autofluorescence in Bacterial Communities
Alma Fernández,
Anton Classen,
Nityakalyani Josyula
et al.
Abstract:The intrinsic fluorescence of bacterial samples has a proven potential for label-free bacterial characterization, monitoring bacterial metabolic functions, and as a mechanism for tracking the transport of relevant components through vesicles. The reduced scattering and axial confinement of the excitation offered by multiphoton imaging can be used to overcome some of the limitations of single-photon excitation (e.g., scattering and out-of-plane photobleaching) to the imaging of bacterial communities. In this wo… Show more
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