Simultaneous treatment of municipal wastewater and biodiesel production by cultivation of Chlorella vulgaris with indigenous wastewater bacteria

Ryu, Byung Gon; Kim, Eun Jung; Kim, Hee Sik; Kim, Jungmin; Choi, Yoon E.; Yang, Ji Won

doi:10.1007/s12257-013-0250-3

Cited by 51 publications

(20 citation statements)

References 35 publications

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“…In addition, Chlorella sp. have been reported to have good nutrient removal potential with sufficient lipid production for bio-fuels from a wide variety of wastewaters (Choi and Lee, 2015;Filippino et al, 2015;Ramanna et al, 2014;Ryu et al, 2014;Xu et al, 2015). Similarly, Scenedesmus sp.…”

Section: Introductionmentioning

confidence: 99%

Dual role of Chlorella sorokiniana and Scenedesmus obliquus for comprehensive wastewater treatment and biomass production for bio-fuels

Gupta

Ansari

Shriwastav

et al. 2016

Journal of Cleaner Production

170

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Microalgal treatment of raw sewage presents many complexities, mainly resulting from the inability of the algal species to sustain increased physiological stresses due to variable nutrient levels and high concentrations of organics. Chlorella sorokiniana and Scenedesmus obliquus have been identified to tolerate higher amounts of organic loading and physiological stresses. Nutrient removal, pathogen removal, and lipid accumulation with secondary or tertiary effluents have been demonstrated independently for these organisms. However, their potentials for accomplishing these objectives simultaneously with raw sewage have not been investigated. This study presents comprehensive investigations of applicability of C. sorokiniana and S. obliquus to wastewater treatment without the requirement for any additional treatment. S. obliquus showed greater potential for removing organic carbon (76.13 ± 1.59% COD removal), nutrients (98.54 ± 3.30% N-removal, 97.99 ± 3.59% P-removal) and comparable pathogens removal (99.93 ± 0.12% total coliforms removal, 100% faecal coliform removal) in comparison to C. sorokiniana (69.38 ± 1.81% COD removal, 86.93 ± 3.49% N-removal, 68.24 ± 11.69% P-removal, 99.78 ± 0.12% total coliforms removal, 100% faecal coliform removal) with 15 days of cultivation with filtered raw sewage, but also encountered increased levels of stress (F v /F m of 0.48 ± 0.03) which accounted for increased lipid accumulation in the cells (23.26 ± 3.95% w/w) but might also affect their biomass productivity and treatment potential in longer applications. In comparison, C. sorokiniana demonstrated better adaptability to physiological stresses (F v /F m of 0.53 ± 0.01) and may be suitable for achieving comprehensive treatment and sufficient lipid accumulation (22.74 ± 3.11% w/w) without compromising these potentials during prolonged applications. These results highlight the importance of selecting algal species with better stress resistance to extend their applicability for comprehensive wastewater treatment and lipid production.

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Section: Introductionmentioning

confidence: 99%

Dual role of Chlorella sorokiniana and Scenedesmus obliquus for comprehensive wastewater treatment and biomass production for bio-fuels

Gupta

Ansari

Shriwastav

et al. 2016

Journal of Cleaner Production

170

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“…1.25-fold increment in lipid content BG-11 (constant stirring at 100 rpm, 25 °C, light intensity of 100 μmol m −2 s −1 ) [ 59 ] Chlorella vulgaris with beneficial bacteria Duganella sp. JPPB B33 (GU368376) and Pseudomonas putida strain MM1 (AY623928) with some other uncultured bacteria Both increment and decrement of biomass productivity due to the influence of uncultured bacteria Wastewater medium with varying bacterial treatments [ 60 ] Ankistrodesmus and Rhizobium 1.29-fold increment in biomass concentration in bubble photobioreactor. 1.19-fold increment in airlift photobioreactor (dry weight) AEX medium; in air bubble photobioreactor [ 61 ] Botryococcus braunii and Candidatus Phycosocius bacilliformis 1.8-fold in the biomass and 1.5-fold improvement in total hydrocarbon yield Vitamin containing modified media of TSP [ 62 ] Microbacterium sp.…”

Section: Resultsmentioning

confidence: 99%

Pelagibaca bermudensis promotes biofuel competence of Tetraselmis striata in a broad range of abiotic stressors: dynamics of quorum-sensing precursors and strategic improvement in lipid productivity

Patidar

Kim

et al. 2018

Biotechnol Biofuels

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BackgroundAmelioration of biofuel feedstock of microalgae using sustainable means through synthetic ecology is a promising strategy. The co-cultivation model (Tetraselmis striata and Pelagibaca bermudensis) was evaluated for the robust biofuel production under varying stressors as well as with the selected two-stage cultivation modes. In addition, the role of metabolic exudates including the quorum-sensing precursors was assessed.ResultsThe co-cultivation model innovated in this study supported the biomass production of T. striata in a saline/marine medium at a broad range of pH, salinity, and temperature/light conditions, as well as nutrient limitation with a growth promotion of 1.2–3.6-fold. Hence, this developed model could contribute to abiotic stress mitigation of T. striata. The quorum-sensing precursor dynamics of the growth promoting bacteria P. bermudensis exhibited unique pattern under varying stressors as revealed through targeted metabolomics (using liquid chromatography–mass spectrometry, LC–MS). P. bermudensis and its metabolic exudates mutually promoted the growth of T. striata, which elevated the lipid productivity. Interestingly, hydroxy alkyl quinolones independently showed growth inhibition of T. striata on elevated concentration. Among two-stage cultivation modes (low pH, elevated salinity, and nitrate limitation), specifically, nitrate limitation induced a 1.5 times higher lipid content (30–31%) than control in both axenic and co-cultivated conditions.ConclusionPelagibaca bermudensis is established as a potential growth promoting native phycospheric bacteria for robust biomass generation of T. striata in varying environment, and two-stage cultivation using nitrate limitation strategically maximized the biofuel precursors for both axenic and co-cultivation conditions (T and T-PB, respectively). Optimum metabolic exudate of P. bermudensis which act as a growth substrate to T. striata surpasses the antagonistic effect of excessive hydroxy alkyl quinolones [HHQ, 4-hydroxy-2-alkylquinolines and PQS (pseudomonas quorum signal), 2-heptyl-3-hydroxy-4(1H)-quinolone].Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1097-9) contains supplementary material, which is available to authorized users.

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“…For the yeast culture grown on primary sludge, the lipid productivity was a bit higher but with overall lower FAMEs content (0.4% (Zhang et al, 2014)). Groups working with primary treated wastewater showed lipid content ranging from 9 to 32% for strains of diverse microbial origin, however, the disclosed content of FAMEs did not exceed 0.3% of dry weight biomass (Bohutskyi et al, 2019;Hall et al, 2011;Ryu et al, 2014;Van Den Hende et al, 2011;Woertz et al, 2009) with the exception of Scenedesmus acutus, where the efficiency of the transesterification reaction was almost 90% (Table 1, (Sacristán de Alva et al, 2013). Microalgae grown on wastewater sampled from the aeration tank of the activated sludge process ('activated wastewater') showed lipid productivity of 0.1 g/L/day, with extractable FAMEs content of 11% from Chlorella sp.…”

Section: Oleaginous Microorganismsmentioning

confidence: 99%

Assessment of the production of biodiesel from urban wastewater-derived lipids

Frkova

Venditti

Herr

et al. 2020

Resources, Conservation and Recycling

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Production of biodiesel is one of the most important European targets within renewables for the future. To consider biodiesel a feasible alternative to fossil fuel, unconventional resources need to be exploited. This review aims to provide up-to-date knowledge on the existing reuse of lipids from urban wastewater to produce biodiesel. Lipids are readily removed by mixed microbial populations during wastewater treatments in sewage plants. Assessment results on potential annual European market supply indicate 3-414 10 4 tons (min for activated and max for grease trap sludge) of potentially extractable biodiesel from wastewater and an expected biodiesel demand of 14.8 10 6 tons. Considering the prospect of transforming sewage plants into biorefineries, we may cover on average 1.5, 6.2, 6.7 and 24.4% of activated, primary, scum and grease trap sludge respectively, of the European biodiesel market from wastewater-derived lipids. In addition, by implementing an optimized biotechnology selector, the overall biodiesel yield could be higher due to increased lipid incorporation into microbial biomass. This is not an insignificant amount and, if efficiently implemented, could represent an exploitable resource for biofuel production, an important and desired step towards a circular economy. The technology readiness level is still very low. There are several challenges and possible drawbacks, e.g., biogas yield loss, substrate depletion, or formation of floating sludge. Finally, no definitive legislative barriers towards wastewater-derived lipids have been identified; however, quality criteria as well as waste status have to be defined. Recently, many reports and regulations have been introducing circular economy principles in areas where sustainability of renewables is strongly considered. Generally, renewable sources having a high environmental impact such as indirect land use change due to crop-to-fuel cultivation, which needs to be better regulated. Accordingly, the limit

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Simultaneous treatment of municipal wastewater and biodiesel production by cultivation of Chlorella vulgaris with indigenous wastewater bacteria

Cited by 51 publications

References 35 publications

Dual role of Chlorella sorokiniana and Scenedesmus obliquus for comprehensive wastewater treatment and biomass production for bio-fuels

Dual role of Chlorella sorokiniana and Scenedesmus obliquus for comprehensive wastewater treatment and biomass production for bio-fuels

Pelagibaca bermudensis promotes biofuel competence of Tetraselmis striata in a broad range of abiotic stressors: dynamics of quorum-sensing precursors and strategic improvement in lipid productivity

Assessment of the production of biodiesel from urban wastewater-derived lipids

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