Simultaneous transcriptome and proteome analysis of EA.hy926 cells under stress conditions induced by nanomaterials

Abstract

Today, the extensive and constantly growing number of applications in the field of nanotechnology poses a lot of questions about the potential toxicity of nanomaterials (NMs) toward cells of different origins. In our work we employed the tools of molecular biology to evaluate changes that occur in human endothelial cells at the transcriptomic and proteomic level, following 24 h of exposure to three different classes of NMs. Using microarray technology, we demonstrated that 24 h of exposure to silver nanoparticles (SNPs), multiwalled carbon nanotubes (MWCNTs) and polyamidoamine dendrimers (PAMAMs) leads to changes in 299, 1271, and 431 genes, respectively, influencing specific molecular pathways. The 2D-DIGE and mass spectrometry analysis revealed that differentially expressed proteins were involved in numerous cellular processes, for example, cytoskeletal reorganization, cell growth and proliferation, or response to stress. Both, transcriptome and proteome alterations indicate reorganization of mechanism regulating cell functioning.

Designing laser‐modified surface structures on titanium alloy custom medical implants using a hybrid manufacturing technology

“…Changes in gene expression in Saos-2 cells were assessed with Agilent SuperPrint G3 Human GE 8×60K V2 Oligonucleotide Microarrays and SureScan Microarray Scanner (Agilent) as described in Komorowski et al (2018). Subsequently data from scanning images were extracted using Feature Extraction Software v. 11.0.1 (Agilent).…”