Simultaneous stereoselective analysis of tramadol and its primary phase I metabolites in plasma by liquid chromatography

Campanero, Miguel Ángel; García-Quetglas, E.; Sádaba, Belén; Azanza, J. R.

doi:10.1016/j.chroma.2003.11.034

Cited by 59 publications

(47 citation statements)

References 17 publications

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“…Os principais efeitos colaterais são a depressão respiratória e os efeitos cardiovasculares. Os estudos atuais revelam que mais de 26 casos de mortes estão associadas à utilização deste fármaco em combinação com os benzodiazepínicos (REITZ et al, 1995), (KOWALUK; ARNERIC, 1998), (FARON-GÓRECKA et al, 2004;CAMPANERO et al, 2004;LEHTONEN et al, 2004;KOROLKOVAS, 1988;CLAROT et al, 2004;PAIVA;LOBO;CHAINHO, 2003;.…”

Section: Derivados Do Ciclohexanolunclassified

Opiáceos, Opióides de ação analgésica e antagonistas

Ferreira

Faccione

2005

Semina: Tech. Ex.

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ResumoOpiáceos e opióides são importantes fármacos utilizados no tratamento da dor. Eles estão relacionados à adicção e a depressão respiratória, efeitos colaterais que limitam o seu uso. Este trabalho descreve as principais classes de fármacos agonistas e antagonistas aos receptores ( , , ). Os principais fármacos são apresentados enfatizando-se relação versus atividade e reações de biotransformações.Palavras-chave: Opiáceos. Opióides de ação analgésica. Relação estrutura. Atividade. Biotransformações. AbstractOpiate and opioid analgesics are important drugs in the treatment of pain. They are associated with addiction and respiratory depression, side effects which limit their clinical usefulness. This paper describes the main classes of agonists and antagonists to opioid receptors ( , , ). The main drugs will be shown emphasizing the structure-activity relationship, as well as biotransformation reactions.

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Section: Derivados Do Ciclohexanolunclassified

Opiáceos, Opióides de ação analgésica e antagonistas

Ferreira

Faccione

2005

Semina: Tech. Ex.

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“…On the other hand, wastewater treatment plants (WWTPs) are unable to completely remove pharmaceutical compounds, and thus their effluents are also sources of these compounds in aquatic systems [2][3][4]. Therefore, residues (i.e., unchanged compounds and their metabolites) are continuously discharged into surface waters, where they can cause toxic effects Different analytical techniques have been reported for the simultaneous quantification of tramadol and its metabolites in biological samples, such as the brain tissue of mice and rats [22], saliva [23], urine [23,24], amniotic fluid [25], plasma [23,[26][27][28][29][30][31][32][33][34][35][36][37][38][39], and environmental samples [11,40], but reports regarding enantiomeric quantification are scarce.…”

Section: Introductionmentioning

confidence: 99%

Enantiomeric Separation of Tramadol and Its Metabolites: Method Validation and Application to Environmental Samples

et al. 2017

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Abstract:The accurate assessment of racemic pharmaceuticals requires enantioselective analytical methods. This study presents the development and validation of an enantioselective liquid chromatography with a fluorescence detection method for the concomitant quantification of the enantiomers of tramadol and their metabolites, N-desmethyltramadol and O-desmethyltramadol, in wastewater samples. Optimized conditions were achieved using a Lux Cellulose-4 column 150 × 4.6 mm, 3 µm isocratic elution, and 0.1% diethylamine in hexane and ethanol (96:4, v/v) at 0.7 mL min −1 . The samples were extracted using 150 mg Oasis ® mixed-mode cation exchange (MCX) cartridges. The method was validated using a synthetic effluent of a laboratory-scale aerobic granular sludge sequencing batch reactor. The method demonstrated to be selective, accurate, and linear (r 2 > 0.99) over the range of 56 ng L −1 to 392 ng L −1 . The detection and the quantification limits of each enantiomer were 8 ng L −1 and 28 ng L −1 for tramadol and N-desmethyltramadol, and 20 ng L −1 and 56 ng L −1 for O-desmethyltramadol. The feasibility of the method was demonstrated in a screening study in influent and effluent samples from a wastewater treatment plant. The results demonstrated the occurrence of tramadol enantiomers up to 325.1 ng L −1 and 357.9 ng L −1 , in the effluent and influent samples, respectively. Both metabolites were detected in influents and effluents.

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“…The spectrofluorimetry [16], HPLC with DAD detection [17], HPLC with UV detection [18][19][20], HPLC with fluorescence detection [21][22][23][24], LC/PDA-ESI-MS/MS [24], potentiometry [25,26], square-wave voltammetric method with amperometric detection [27], gas chromatography with mass spectrophotometer [28,29], gas chromatography with nitrogen-phosphorus detection [30], HPTLC [31][32][33] methods reported for the determination of TDL are sensitive enough but require expensive or sophisticated instruments, involve procedures with rigorous control of the experimental conditions, require long and tedious pretreatment of the samples and laborious clean up procedures prior to analysis and are not simple for routine analysis. However, spectrophotometric methods are particularly attractive because of ease in accessibility and their quick applicability to routine analysis.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Assay of Opioid Analgesic, Tramadol, Using Horseradish Peroxidase

N¹,

Srilakshmi

Alekya³

et al. 2014

ILNS

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Three novel and sensitive enzymatic methods have been developed for the quantification of tramadol in pharmaceutical dosage forms. The proposed methods are based on the reaction of tramadol with 3-methylbenzothiazoline-2-one hydrazone (method A), aniline (method B) and aminoantipyrine in the presence of hydrogen peroxide and horseradish peroxidase to give colored complexes. The colored complexes obtained with 3-methylbenzothiazoline-2-one hydrazone, aniline and aminoantipyrine exhibit absorption maxima at 480 nm, 550 nm and 530 nm, respectively. Regression analysis of Beer's plots showed good correlation for tramadol in the concentration range (μg/mL) 2-12 for the methods A & B and 4-24 for the method C. The experimental parameters were studied and optimized. The precision and accuracy of the methods were satisfactory. The proposed methods were successfully applied for the quantification of tramadol in 3 brands of commercially available tablet dosage forms. The results were compared satisfactorily with the official method.

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Simultaneous stereoselective analysis of tramadol and its primary phase I metabolites in plasma by liquid chromatography

Cited by 59 publications

References 17 publications

Opiáceos, Opióides de ação analgésica e antagonistas

Opiáceos, Opióides de ação analgésica e antagonistas

Enantiomeric Separation of Tramadol and Its Metabolites: Method Validation and Application to Environmental Samples

Enzymatic Assay of Opioid Analgesic, Tramadol, Using Horseradish Peroxidase

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