Simultaneous Spectrophotometric Determination of Ternary Mixtures of Tartrazine, Sunset Yellow, and Ponceau 4r by H-Point Standard Addition Method

Ni, Yongnian; Qi, Ming; Kokot, Serge

doi:10.1081/al-100107537

Cited by 15 publications

(5 citation statements)

References 24 publications

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“…Importantly, this technique can be used to extract independent flow rates from multiple inputs/channels in a single measurement using dyes with reasonably resolved absorption maxima (l max ) and spectral deconvolution. Food dye mixtures that have been previously quantified and resolved optically [38][39][40] are ideal for this type of measurement because of their: (1) high solubility in water (and therefore low PDMS partitioning [Fig. S2 and S3 †]), (2) absorption in the visible part of the spectrum, and (3) compatibility with a wide range of microfluidic substrates.…”

Section: Introductionmentioning

confidence: 99%

A simple method for the evaluation of microfluidic architecture using flow quantitation via a multiplexed fluidic resistance measurement

et al. 2010

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Quality control of microdevices adds significant costs, in time and money, to any fabrication process. A simple, rapid quantitative method for the post-fabrication characterization of microchannel architecture using the measurement of flow with volumes relevant to microfluidics is presented. By measuring the mass of a dye solution passed through the device, it circumvents traditional gravimetric and interface-tracking methods that suffer from variable evaporation rates and the increased error associated with smaller volumes. The multiplexed fluidic resistance (MFR) measurement method measures flow via stable visible-wavelength dyes, a standard spectrophotometer and common laboratory glassware. Individual dyes are used as molecular markers of flow for individual channels, and in channel architectures where multiple channels terminate at a common reservoir, spectral deconvolution reveals the individual flow contributions. On-chip, this method was found to maintain accurate flow measurement at lower flow rates than the gravimetric approach. Multiple dyes are shown to allow for independent measurement of multiple flows on the same device simultaneously. We demonstrate that this technique is applicable for measuring the fluidic resistance, which is dependent on channel dimensions, in four fluidically connected channels simultaneously, ultimately determining that one chip was partially collapsed and, therefore, unusable for its intended purpose. This method is thus shown to be widely useful in troubleshooting microfluidic flow characteristics.

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Section: Introductionmentioning

confidence: 99%

A simple method for the evaluation of microfluidic architecture using flow quantitation via a multiplexed fluidic resistance measurement

et al. 2010

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“…These effects make knowledge about food components an important issue for the detection of and information on the synthetic colorants in foodstuffs and beverages. Towards developing this knowledge, many analytical methods have been developed using spectrometric determination (Blanco, Campana, & Barrero, 1996;El-Sheikh & Al-Degs, 2013;Ni, Qi, & Kokot, 2001;Santos, Demiate, & Nagata, 2010), Raman spectroscopic (Uhlemanna et al, 2012), voltammetry (Gan, Sun, Meng, Song, & Zhang, 2013;Ghoreishi et al, 2012;Ni, Bai, & Jin, 1997), capillary electrophoresis (Huang, Chiu, Sue, & Cheng, 2003;López-Montes, Dupont, Desmazières, & Lavédrine, 2013;Prado, Boas, Bronze, & Godoy, 2006), and high performance liquid chromatography with diode array detection (HPLC-DAD) (Kirschbaum, Krause, Pfalzgraf, & Bruckner, 2003) for synthetic colorant determination in foodstuffs and beverages. These techniques require prior sample preparation and can be used anywhere from a simple dilution followed by filtration up to more advanced techniques such as solid phase extraction (SPE) through centrifugation and sonication of samples to extract all the colorant.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of synthetic colorants in yogurt by HPLC

2015

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“…HPSAM can resolve spectra of two analytes with strongly overlapping spectra. This method also uses multicomponent analysis (the mathematical details of which have been described elsewhere [21][22][23]). Vardu-Andres et al [24] and Campins-Falco et al [25] have extended the HPSAM to resolve ternary mixtures.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous spectrophotometric determination of phenolic antioxidant (BHA and BHT) concentrations in pharmaceutical preparations and chewing gums using the H-point standard addition method

Özgür

Kalaycıoğlu

Dülger

2017

Maced. J. Chem. Chem. Eng.

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A simple, rapid, and sensitive spectrophotometric method was developed for the simultaneous determination of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) concentration in pharmaceutical preparations and chewing gums, without prior separation steps, using the H-point standard addition method (HPSAM).The concentration of one antioxidant was calculated by overlapping spectra at two appropriately selected wavelengths at which the interferent, other antioxidant, should has the same absorbance value. Absorbances at two pairs of wavelengths, 265 and 288 nm (with BHA as analyte) or 288 and 293 nm (with BHT as analyte) were monitored, while adding standard solutions of BHA or BHT, respectively. Calibration graphs were determined at 4–20 μgml–1(r = 0.9981) for BHA and 20–100 μgml–1(r = 0.9940) for BHT in binary mixtures. The proposed method was tested and validated using various parameters according to ICH guidelines. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.48 and 1.51 μgml–1for BHA and 0.72 and 2.41 μgml–1for BHT, respectively. The percentage recovery ranges were 100.44–102.50 % for BHA and 96.45–100.04 % for BHT, with relative standard deviations (RSD) less than 1.48 % indicating reasonable repeatability of the method. The intra-day and inter-day precision tests showed reliable RSD values (< 2 %). The results obtained using HPSAM were statistically compared with results obtained using the derivative spectrophotometric method that was previously reported by us, showing high similarity between results.

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Simultaneous Spectrophotometric Determination of Ternary Mixtures of Tartrazine, Sunset Yellow, and Ponceau 4r by H-Point Standard Addition Method

Cited by 15 publications

References 24 publications

A simple method for the evaluation of microfluidic architecture using flow quantitation via a multiplexed fluidic resistance measurement

A simple method for the evaluation of microfluidic architecture using flow quantitation via a multiplexed fluidic resistance measurement

Simultaneous determination of synthetic colorants in yogurt by HPLC

Simultaneous spectrophotometric determination of phenolic antioxidant (BHA and BHT) concentrations in pharmaceutical preparations and chewing gums using the H-point standard addition method

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