Simultaneous Spectrophotometric Determination of Sunset Yellow and Quinoline Yellow in a Single Step

Abstract

In this work a new modified standard addition method for simultaneous spectrophotometric determination of Sunset yellow (SY) and Quinoline yellow (QY) is used. The generalized Net Analyte Signal Standard Addition Method (GNASSAM) was introduced for simultaneous standard addition of analytes. By applying this method the concentrations of analytes are determined in a single step. The applicability of new method was used for the simultaneous determination of strongly overlapped spectra of SY and QY in a real sample without using any separation step. GNASSAM was also used to calculate figures of merit. Moreover, good limits of detection (SY = 0.13 and QY = 0.16 mgL -1 ) and suitable selectivity and sensitivity were determined. HPLC method was applied for finding subspace in binary mixtures of real samples. The results of investigated method for real sample were compared with HPLC results. The obtained results were in a good agreement with those of HPLC method.

A Simple and Efficient Turn‐Off Fluorescence Sensor for the Nanomolar Detection of Homovanillic Acid Using Protein Mediated Blue Emitting Nickel Nanoclusters

“…No significant change in the life time of BSA NiNCs was observed during the lifetime measurement on adding 5.00×10 <‐M‐>7 M HVA to it (Figure 7b). In this work, the obtained ratio of the life time of fluorescent probe in the absence (τ 0 ) and presence of quencher (τ) was one (τ 0 /τ=1) which supports the possibility for a static quenching mechanism and also ruled out a chance for the occurrence of FRET [21] . The absence of an overlap in the emission spectra of fluorophore with absorption spectra of HVA in Figure 7d further confirms the absence of a FRET and inner filter effect [22] .…”

A Zinc Oxide Nanoflower-Based Electrochemical Sensor for Trace Detection of Sunset Yellow

“…Consequently, a simple, rapid, and highly sensitive method for SY detection is critical to monitor SY levels. Many techniques have been utilized for the determination of SY, such as high performance liquid chromatography [3,4,5], UV-visible, fluorescence, and Raman spectrometry [6,7,8,9], and electrochemical techniques [10,11,12,13,14,15,16,17]. Recently, due to the low cost, operational simplicity, high sensitivity, and fast response, electrochemical techniques have gained attention and have exhibited promising applications in SY analysis.…”

Determination of the colorants in a beverage sample by chemometrıc methods using an ultraviolet spectrophotometer

“…Bu tür numunelerin ayrı ayrı numunelerde analizi için farklı enstrümental tekniklerin kullanıldığı çalışmalarda mevcuttur. βkaroten, spektrofotomerik [8] olarak; sunset yellow, spektrofotometrik olarak [9,10,11], elektrokimyasal olarak [12,13], kolorimetrik olarak [14], yüksek performans sıvı kromatografisi ile [15], floresans yöntemi [16], raman spektroskopisi [17], viskozite [18] ve adsorpsiyon tekniği [19] ile incelenmişlerdir. Literatür incelendiğinde sunset yellow ve β-karotenin birlikte eş zamanlı tayinine rastlanmamıştır.…”