Simultaneous simple and fast quantification of three major immunosuppressants by liquid chromatography—tandem mass-spectrometry

Volosov, Andrew; Napoli, Kimberly L.; Soldin, Steven J.

doi:10.1016/s0009-9120(01)00235-1

Cited by 106 publications

(55 citation statements)

References 30 publications

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“…Under these simplified chromatographic conditions, CsA and CsD co-eluted with the unspecified, more hydrophilic metabolites of CsA; one single peak was found and integrated in both MRM traces ( Figure 1C, D), both in calibration samples and patient samples. The retention time of this single peak was approximately 2.1 min, which is in agreement with previously described methods for the quantification of CsA employing CsD as the internal standard and the same mass transitions as applied here (2)(3)(4)(5). The quantitative results obtained in this series using limited chromatography differed markedly from those obtained with extended chromatography and from the immunoassay results (Table 1).…”

supporting

confidence: 89%

Pitfall in the high-throughput quantification of whole blood cyclosporin A using liquid chromatography-tandem mass spectrometry

Vogeser

Spohrer²

2005

Clinical Chemistry and Laboratory Medicine (CCLM)

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In a growing number of laboratories the technique of liquid chromatography-tandem mass spectrometry is used for the quantification of cyclosporin A in whole blood, employing cyclosporin D as the internal standard. Cyclosporin A is extensively metabolized in vivo; in liquid chromatography-tandem mass spectrometry respective metabolites can give rise to both parent and product ions that are isobaric with ions commonly used for the detection of cyclosporin A and cyclosporin D, respectively. In this article it is demonstrated that limited chromatography with co-elution of such metabolites together with cyclosporin A and cyclosporin D can lead to incorrect results.Keywords: cyclosporin A; cyclosporin D; liquid chromatography-tandem mass spectrometry; specificity.In tandem-mass spectrometry the detection of target analytes is based on their physical decomposition behavior; the molecular mass-specific selection of an intact precursor ion of an analyte and of the respective product ion results in very high analytical specificity. This high degree of specificity can limit the requirements of sample preparation and chromatography and may enable ''high-throughput'' routine methods. However, the specificity of tandem-mass spectrometry should not be assumed to be absolute and unlimited. Given the complexity of human body fluids as a matrix and the extensive metabolization of certain target analytes, the specificity of liquid chromatography-tandem mass spectrometry (LC-MS/MS) may be a critical issue. This is demonstrated by an observation reported here.With the intention of switching the quantification of whole blood cyclosporin A (CsA) from immunoassay technology to LC-MS/MS, we decided to modify the LC-MS/MS method that we currently use for the quan-

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supporting

confidence: 89%

Pitfall in the high-throughput quantification of whole blood cyclosporin A using liquid chromatography-tandem mass spectrometry

Vogeser

Spohrer²

2005

Clinical Chemistry and Laboratory Medicine (CCLM)

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“…The protocol for CyA determination was based on previously established methodology (Volosov et al, 2001). Blood samples (1 ml) were collected into microcentrifuge tubes containing 3.2 mg of EDTA and were stored at Ϫ20°C until preparation for liquid chromatography-mass spectrometry.…”

Section: Methodsmentioning

confidence: 99%

BRAIN CYCLOSPORIN A LEVELS ARE DETERMINED BY ONTOGENIC REGULATION OFMDR1AEXPRESSION

Goralski

Acott²,

Fraser³

et al. 2005

Drug Metab Dispos

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“…However, in hospitals mass spectrometry is often not available for afterhours use. Although major improvements in mass spectrometry methods have simplified sample preparation and provided faster turnaround times, the above-mentioned limitations still persist (10 ).…”

Section: 2*mentioning

confidence: 99%

Tacrolimus Measurement: Building a Better Immunoassay

Gounden

Soldin

2014

Clinical Chemistry

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Simultaneous simple and fast quantification of three major immunosuppressants by liquid chromatography—tandem mass-spectrometry

Cited by 106 publications

References 30 publications

Pitfall in the high-throughput quantification of whole blood cyclosporin A using liquid chromatography-tandem mass spectrometry

Pitfall in the high-throughput quantification of whole blood cyclosporin A using liquid chromatography-tandem mass spectrometry

BRAIN CYCLOSPORIN A LEVELS ARE DETERMINED BY ONTOGENIC REGULATION OFMDR1AEXPRESSION

Tacrolimus Measurement: Building a Better Immunoassay

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