Simultaneous Separation of Ionic and Nonionic Compounds Using Reversed-Phase Micellar Chromatography

Okada, Tetsuo

doi:10.2116/analsci.9.59

Cited by 8 publications

(1 citation statement)

References 16 publications

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“…These observations mainly reflect different partition natures of these solutes. The partition coefficients between water and the SDS micelle have been reported to be 40.7−44.7, 219−243, and 607 for phenol, p -chlorophenol, and naphthol, corresponding to α = 0.51−0.53, 0.85−0.87, and 0.94, respectively. , Horizontal lines in Figure A show α values for these phenolic solutes in 100 mM SDS solution. Comparison of the elution profiles depicted in Figure with Figure allows us to evaluate their D 2 values in aqueous solutions.…”

Section: Resultsmentioning

confidence: 97%

Resolution of Small Molecules by Passage through an Open Capillary

2005

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When a solute passes through an open capillary, in which a laminar flow is established, different peak profiles can be obtained according to its diffusion property under a working condition, i.e., the radius and length of the capillary and the flow rate of the carrier solution. If a solute diffuses over the entire cross section of the capillary before it is eluted, a Gaussian-shaped diffusion peak appears, which has an apex at the travel time of the average flow. Insufficient solute diffusion, which is realized, e.g., by increasing flow rates or capillary radius, produces a new peak having an apex at the travel time of the maximum flow. This implies that two solutes can be resolved simply by passing through a capillary. However, our previous study indicated that the diffusion coefficients of two solutes should be at least one order different for their resolution based on this principle, suggesting that its applicability is highly restricted. In the present paper, this concept has been extended to the resolution between dissolved solutes that have similar intrinsic diffusion properties. The incorporation of molecular aggregates in the carrier makes a solute less diffusive according to the extent of their interaction and allows the resolution of a dissolved molecule from other ones differing in the affinity to the molecular aggregates. Several examples of peak resolution for phenols, aromatic hydrocarbons, and inorganic anions are shown and discussed on the basis of the modification of the diffusion natures due to their interactions with micelles or vesicles.

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Section: Resultsmentioning

confidence: 97%

Resolution of Small Molecules by Passage through an Open Capillary

2005

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Separation of mercury(II), methylmercury and phenylmercury by micellar high-performance liquid chromatography on short columns

Hutta

Megová

Halko

1998

J Radioanal Nucl Chem

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Three environmentally and agrochemicaly important mercury species: methylmercury, phenylmercury and mercury(II) are separated within 4 minutes as bromocomplexes by micellar liquid chromatography using very short reversed-phase (RP) C18 columns (up to 30 mm). The micellar mobile phase containig 0.05M cetyltrimethylammonium bromide (CTMA+Br), 1% (v/v) 2-propanol, 0.001M cyclohexylenediaminetetraacetic acid (DCTA) and sulfuric acid (pH 2) showed good selectivity in mixed reversed-phase and anion-exchange mode. The above mentioned separation order in which organomercurials are eluted far behind the void volume of the column, but before the mercury(II) peak is advantageous in all instances where mercury(II) is present in real samaples in great excess. Environmental and agrochemical samples contain humic material which does not interfere in this particular system. The low cost photometric detection at 500 nm after post-column derivatization by CTMA+Br micellized dithizone is almost free from interferences and enables detection limits at the 14 ng level (e.g., 0.1 ppm Hg) for 20 ~tl samples.

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Multifunctional separation with polyamine-bonded resin

Okada

1995

Analytica Chimica Acta

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Simultaneous Separation of Ionic and Nonionic Compounds Using Reversed-Phase Micellar Chromatography

Cited by 8 publications

References 16 publications

Resolution of Small Molecules by Passage through an Open Capillary

Resolution of Small Molecules by Passage through an Open Capillary

Separation of mercury(II), methylmercury and phenylmercury by micellar high-performance liquid chromatography on short columns

Multifunctional separation with polyamine-bonded resin

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