Simultaneous separation of hydrophobic and hydrophilic peptides with a silica hydride stationary phase using aqueous normal phase conditions

Boysen, Reinhard I; Yang, Yanrong; Chowdhury, Jamil; Matyska, Maria T.; Pesek, Joseph J.; Hearn, Milton Thomas William

doi:10.1016/j.chroma.2011.09.009

Cited by 33 publications

(23 citation statements)

References 16 publications

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“…peptide retention was enhanced as the amount of the less polar component (ACN) of the mobile phase was increased. The retention behavior of these peptides on the DH column reflected that observed for other previously investigated small polar compounds, such as amino acids, carbohydrates, organic acids, and nucleotides . As shown in Fig.…”

Section: Resultssupporting

confidence: 72%

“…The low percentage (∼2%) of bonded carbon on the surface has been shown to offer better separation efficiencies and peak symmetry for compounds without a significant change in their retention times . In a preliminary study, strong retention of polar peptides on the DH phase was observed under some conditions . It was therefore of interest to systematically explore the effects of different mobile phase compositions on the retention of peptide analytes using this DH phase.…”

Section: Resultsmentioning

confidence: 99%

“…It was employed in our previous studies for the ANP analysis of small polar compounds, including amino acids, carbohydrates, and organic acids . In another investigation, this mobile phase additive was used for the retention and simultaneous separation of both hydrophobic and hydrophilic peptides with a DH column . Figure illustrates the retention plots of the selected peptides on the DH column with 0.1% v/v AA present as the mobile phase additive.…”

Section: Resultsmentioning

confidence: 99%

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Analysis of polar peptides using a silica hydride column and high aqueous content mobile phases

et al. 2013

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The retention behavior of a set of polar peptides separated on a silica hydride stationary phase was examined with a capillary HPLC system coupled to ESI-MS detection. The mobile phases consisted of formic acid or acetic acid/acetonitrile/water mixtures with the acetonitrile content ranging from 5 to 80% v/v. The effects on peptide retention of these two acidic buffer additives and their concentrations in the mobile phase were systematically investigated. Strong retention of the peptides on the silica hydride phase was observed with relatively high-organic low-aqueous mobile phases (i.e. under aqueous normal-phase conditions). However, when low concentrations of acetic acid were employed as the buffer additive, strong retention of the peptides was also observed even when high aqueous content mobile phases were employed. This unique feature of the stationary phase therefore provides an opportunity for chromatographic analysis of polar peptides with water-rich eluents, a feature usually not feasible with traditional RP sorbents, and thus under conditions more compatible with analytical green chemistry criteria. In addition, both isocratic and gradient elution procedures can be employed to optimize peptide separations with excellent reproducibility and resolution under these high aqueous mobile phase conditions with this silica hydride stationary phase.

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Section: Resultssupporting

confidence: 72%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Analysis of polar peptides using a silica hydride column and high aqueous content mobile phases

et al. 2013

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“…Here a mixture of peptides ranging from very hydrophobic to . very hydrophilic are separated in a single run on the same Diamond Hydride column [37]. Such a result is not possible on other columns and in fact most proteomic analyses are done using a twodimensional approach; one run done on a hydrophobic phase such as C18 and the second run done on a more polar medium such as ion-exchange.…”

Section: Separation Mechanisms On Silica Hydridementioning

confidence: 99%

The Development of Silica Hydride Stationary Phases for High Performance Liquid Chromatography from Conception to Commercialization

Matyska¹,

Pesek²

2019

Preprint

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The development of a stationary phase material for high performance liquid chromatography based on a surface of silica hydride as opposed to silanols on ordinary silica is discussed including synthetic approaches, characterization and applications. There are several synthetic approaches available to create a silica hydride surface. Modification of the Si-H moiety on the silica surface can be accomplished through use of a hydrosilation reaction. Both the intermediate silica hydride and the material modified with an organic moiety can be characterized by a number of spectroscopic as well as a variety of other methods. Further insights into the retention mechanism are provide through chromatographic measurements. The ultimate utility of any chromatographic stationary phase material is determined by its success for solving challenging analytical problems. A broad range of applications are reviewed to illustrate the versatility and usefulness of silica hydride-based stationary phases.

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“…14,16,17 The silica hydride surface possess nearly 10 times less silanol activity as compared to an endcapped Type B silica. 14,15,17,18 Figures 7a and 7b below depict the structural differences between Type B and C silica. 15 The lack of a polar group on the silica surface in Type C silica also resulted in little or no water adsorption, thus making it a good choice for organic normal phase chromatography with a variety of solvents and improved speed.…”

Section: F Type C Silica-silica Hydridementioning

confidence: 99%

Evaluation of a Silica Hydride-Based Undecynoic Acid Stationary Phase For High Performance Liquid Chromatography

Kozhikote¹

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Simultaneous separation of hydrophobic and hydrophilic peptides with a silica hydride stationary phase using aqueous normal phase conditions

Cited by 33 publications

References 16 publications

Analysis of polar peptides using a silica hydride column and high aqueous content mobile phases

Analysis of polar peptides using a silica hydride column and high aqueous content mobile phases

The Development of Silica Hydride Stationary Phases for High Performance Liquid Chromatography from Conception to Commercialization

Evaluation of a Silica Hydride-Based Undecynoic Acid Stationary Phase For High Performance Liquid Chromatography

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