Simultaneous separation and identification of cyanocobalamin, thiamine, and ascorbic acid on polyoxyethylene sorbitan monooleate-impregnated silica layers with water as mobile phase

Mohammad, Ali; Zehra, Almas

doi:10.1556/jpc.22.2009.6.8

Cited by 6 publications

(1 citation statement)

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“…The synergistic effect of two surfactants, SDS in stationary phase and Tween 80 as the component of the mobile phase TLC systems, was examined by Mohammad and Zehra to separate water-soluble vitamins 28 . The same authors distinguished cyanocobalamin, thiamine, and ascorbic acid using TLC systems with Tween 80-impregnated silica layers and water as the mobile phase 29 .…”

Section: Introductionmentioning

confidence: 99%

Separation of some vitamins in reversed-phase thin-layer chromatography and pressurized planar electrochromatography with eluent containing surfactant

Polak

Pajurek

2021

Sci Rep

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The separation of some water- and fat-soluble vitamins via micellar systems of reversed-phase high-performance thin-layer chromatography (HPTLC) and pressurized planar electrochromatography (PPEC) was subjected to research. Hence, the influence of the mobile phase composition (surfactant and acetonitrile concentration, eluent buffer pH) on the migration distances and zone separation of some vitamins (thiamine, riboflavin, niacin, pyridoxine, cyanocobalamin, folic acid, ergocalciferol and α-tocopherol) was investigated. Our results indicated that the applied technique has an impact on the solute order. Comparing the system capacity of HPLC and PPEC (measured as height of the theoretical plate) for the mobile phase systems with and without surfactant shows differences, especially for fat-soluble vitamin. The variances and reproducibilities (% RDS) values of the vitamin are less in PPEC than in TLC. Moreover, the migration distances of water-soluble vitamins are longer than fat-soluble ones. Overall, eluent consisting of 50% acetonitrile, 18.75 mM SDS, the buffer of pH 6.99 via the PPEC technique was most appropriate for determining the investigated vitamins in the artificial mixture and the two commercially available vitamin combinations.

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Section: Introductionmentioning

confidence: 99%