Simultaneous Rp-HPLC Determination of Cefpodoxime Proxetil and Ofloxacin

Abstract

A rapid and simple high Reverse-phase performance liquid chromatography method was developed and validated for simultaneous determination of Cefpodoxime Proxetil and Ofloxacin from pharmaceutical preparation. The separation was achieved on A kromosil (technokroma) 100 C-8 (3.5μm ,15x0.46) performed using Acetonitrile and phosphate buffer as mobile phase the of pH of buffer was adjusted to 4.8 using Ortho-phosphoric acid, the ratio was 58:42 (v/v) eluents were monitored at 275 nm. The detector response was linear in the range of 100 to 300 μg, for both Cefpodoxime Proxetil and Ofloxacin. The percentage assay Cefpodoxime Proxetil and Ofloxacin was found between 99.76 and 99.31% respectively. The described method has the advantage of being rapid and easy hence it can be applied for routine quality control analysis.

Ecological spectroscopic methodologies for quantifying co-administered drugs in human plasma by photochemical quantum mechanical simulation

“…Literature review revealed several spectroscopic [ 9 , 10 ] and chromatographic [ 11 , 12 ] methods for the determination of OFL in combination of either FLV or CPD binary mixtures. These methods were defective in determining the three drugs in their potential future fixed dose combinations or in plasma after concomitant administration.…”