Simultaneous RP HPLC Determination of Aceclofenac, Paracetamol and Tizanidine in Pharmaceutical Preparations

Vaidya, Vikas V.; Singh, GN; M.P.Choukekar,; M.B.Kekare,

doi:10.1155/2010/323410

Cited by 15 publications

(11 citation statements)

References 5 publications

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“…The low recovery of colchicine may be affected by the presence of the other constituents in the tablet sample. The t-test statistics reveals that for recovery results in this study for ampicillin and paracetamol are in a good agreement with the work of Barot et al 33 and Avidya et al 34 who used HPLC technique for ampicillin and paracetamol respectively.…”

Section: Recovery Testsupporting

confidence: 90%

Ion Chromatographic Determination of Colchicine, Ampicillin and Paracetamol in Pharmaceutical Preparations and Urine Matrix

Khammas¹,

Sonnenschein²,

Seubert³

2011

JCPR

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A new, simple, rapid and inexpensive ion chromatographic method with UV detection has been established for the separation and simultaneous determination of colchicine, ampicillin and paracetamol in pure form and pharmaceutical preparations individually and in mixture. The method is based on using of weak and strong cation exchanger columns (WCX and SCX). The retention mechanisms for the selected drugs for both columns have also been investigated; thereby their retention parameters were quantified at optimum conditions. The ion chromatographic method was validated for linearity, limit of detection, limit of quantification, precision and recovery percent. Linearity was found in the range of 5-200 mg L-1 for colchicine and of 2.5-200 mg L-1 , for ampicillin and paracetamol, with detection limits of, 0.92, 2.08 and 2.06 mg L-1 and recovery percent of 94±0.05%, 99.95±0.5% and 104.8±4.6%, for colchicine, ampicillin and paracetamol respectively. The proposed method was successfully applied for the direct determination of colchicine in urine sample using "dilute and shoot "approach.

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Section: Recovery Testsupporting

confidence: 90%

Ion Chromatographic Determination of Colchicine, Ampicillin and Paracetamol in Pharmaceutical Preparations and Urine Matrix

Khammas¹,

Sonnenschein²,

Seubert³

2011

JCPR

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“…Methanol and water (70:30 v/v) had been used in RP-HPLC method for aceclofenac and paracetamol assay in 2 to 50 µg/mL concentration range for Aceclofenac (Godse et al, 2009). This technique has also been applied for assay calculation of aceclofenac, paracetamol and tizanidine with mobile phase as phosphate buffer pH 7.0: acetonitrile (40:60 v/v) at 0.7 mL/min flow rateand UV detection at 230 nm (Vaidya et al, 2010). In another study, acetonitrile: water: 0.025 M buffer of potassium dihydrogen orthophosphate (70:10:20% v/v/v) having pH 3.0 stabilized by orthophosphoric acid was used for assay of aceclofenac and thiocolchicoside using spectrophotometry and liquid chromatography.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of UV- Spectrophotometric and RP-HPLC method for the analysis of raw material and formulations of Aceclofenac

Jamshaid¹,

Hassan²,

Ahmed³

et al. 2020

Afr. J. Pharm. Pharmacol.

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The purpose of the study is to develop and validate method for assay of Aceclofenac in tablet dosage forms using ultra violet spectrophotometry (UV) and high performance liquid chromatography (HPLC) techniques. A method was developed and validated for analysis of Aceclofenac using UV technique with methanol and phosphate buffer 7.4 as solvent. The HPLC analysis was conducted using two mobile phases, that is, "A" as Acetonitrile: Methanol (80:20 v/v) and "B" as Acetonitrile: methanol: NH 3 solution (225:50:1 v/v/v). The method was used for assay determination for tablets dosage forms and results were found to be in compliance with official standards. Validation studies were also carried out for both methods. Linearity, LOD, single point calibration, precision and accuracy and % RSD were calculated. Aceclofenac standard was analysed with UV Spectrophotometer in the concentration ranges of 0.5-50 and 0.4-50 mg/L for each solvent and results showed good linearity with R 2 = 0.9998 and 0.9999. The method was also specific that verifies the absence of interference at the  max of Aceclofenac. UV analysis was precise with % RSD falling within 2% and LOD as 0.5 and 0.4 mg/L for methanol and PBS 7.4, respectively. The tablets of three brands showed assay percentages within specified limits in methanol (109.33, 103.90 and 105.61%) and PBS 7.4 (108.70, 100.69 and 106.60%). In HPLC analysis, mobile phase "B" showed more sharp peaks with lesser HETP and T f compared to mobile phase "A". The method was checked for reliability and efficiency for assay and some of the parameters like height efficiency to theoretical plates (HETP), tailing factor, peak heights, peak widths along with validation studies (Linearity range 0.1-50 mg/L, specificity, precision, and limit of detection and single point calibration). The more basic mobile phase B using NH 3 solution produced more sharp peaks as compared to less basic mobile phase A.

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“…United States Pharmacopeia (USP) have analytical methodologies involving TZ, using high performance liquid chromatography (LC) for related compounds determination (USP 39, 2016). Studies using LC have been reported with tizanidine alone or in association with other drugs, but there isn`t one using association with charged aerosol detector (CAD) (2,(5)(6)(7)(8). CAD detector is a new type of detector introduced for LC applications.…”

Section: Introductionmentioning

confidence: 99%

Stability-Indicating Method for Determination of Tizanidine Hydrochloride in Pharmaceutical Lc-Cad Method Using Chemometric Approach

et al. 2017

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A new LC method for tizanidine hydrochloride in tablet dosage form using a charged aerosol detector (CAD) is described. The influence of various parameters on chromatographic system was investigated by factorial designs and Derringer's desirability. Chromatographic conditions were: mobile phase constituted of acetonitrile and ammonium acetate buffer 17 mM (60:40; v/v), column oven at 39 °C and flow rate 0.8 mL.min-1 performed in on Acclaim Trinity P1 column UV at 230 nm. Thus, it was possible to validate a simple method to assay the tizanidine and its counter-ion in three formulations (drug reference, generic and manipulated). Method showed specificity when challenged by forced degradation and excipients. Finally, the method was compared with USP monograph method demonstrating equivalence in assay evaluation.

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Simultaneous RP HPLC Determination of Aceclofenac, Paracetamol and Tizanidine in Pharmaceutical Preparations

Cited by 15 publications

References 5 publications

Ion Chromatographic Determination of Colchicine, Ampicillin and Paracetamol in Pharmaceutical Preparations and Urine Matrix

Ion Chromatographic Determination of Colchicine, Ampicillin and Paracetamol in Pharmaceutical Preparations and Urine Matrix

Development and validation of UV- Spectrophotometric and RP-HPLC method for the analysis of raw material and formulations of Aceclofenac

Stability-Indicating Method for Determination of Tizanidine Hydrochloride in Pharmaceutical Lc-Cad Method Using Chemometric Approach

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