Simultaneous Respiratory Exchange and Blood Sugar Time Curves

Rabinowitch, I. M.

doi:10.1001/archsurg.1933.01170040159012

Cited by 5 publications

(3 citation statements)

References 7 publications

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“…Insulin is typically applied at 0.03 U in 20 μL saline to treat excisional wounds in mice, which is equivalent to 52.05 ng/mL, while our study reports accelerated wound healing at much lower concentration. 9 , 30 …”

Section: Discussionmentioning

confidence: 99%

“…Rabinowitch reported disturbed sugar metabolism in nondiabetic patients presenting with slow wound healing arrested in the inflammatory phase. 8 , 9 Blood samples in these patients did not meet the diagnostic criteria for diabetes, but abnormal blood sugar time curves were observed. Their findings suggest that carbohydrate metabolism may be disturbed during wound healing and may actually be impairing wound repair when infection is present.…”

Section: Introductionmentioning

confidence: 97%

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Hydrogel Microencapsulated Insulin-Secreting Cells Increase Keratinocyte Migration, Epidermal Thickness, Collagen Fiber Density, and Wound Closure in a Diabetic Mouse Model of Wound Healing

Aijaz

Faulknor

Berthiaume

et al. 2015

Tissue Engineering Part A

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Wound healing is a hierarchical process of intracellular and intercellular signaling. Insulin is a potent chemoattractant and mitogen for cells involved in wound healing. Insulin's potential to promote keratinocyte growth and stimulate collagen synthesis in fibroblasts is well described. However, there currently lacks an appropriate delivery mechanism capable of consistently supplying a wound environment with insulin; current approaches require repeated applications of insulin, which increase the chances of infecting the wound. In this study, we present a novel cell-based therapy that delivers insulin to the wound area in a constant or glucose-dependent manner by encapsulating insulin-secreting cells in nonimmunogenic poly(ethylene glycol) diacrylate (PEGDA) hydrogel microspheres. We evaluated cell viability and insulin secretory characteristics of microencapsulated cells. Glucose stimulation studies verified free diffusion of glucose and insulin through the microspheres, while no statistical difference in insulin secretion was observed between cells in microspheres and cells in monolayers. Scratch assays demonstrated accelerated keratinocyte migration in vitro when treated with microencapsulated cells. In excisional wounds on the dorsa of diabetic mice, microencapsulated RIN-m cells accelerated wound closure by postoperative day 7; a statistically significant increase over AtT-20ins-treated and control groups. Histological results indicated significantly greater epidermal thickness in both microencapsulated RIN-m and AtT-20ins-treated wounds. The results suggest that microencapsulation enables insulin-secreting cells to persist long enough at the wound site for a therapeutic effect and thereby functions as an effective delivery vehicle to accelerate wound healing.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Hydrogel Microencapsulated Insulin-Secreting Cells Increase Keratinocyte Migration, Epidermal Thickness, Collagen Fiber Density, and Wound Closure in a Diabetic Mouse Model of Wound Healing

Aijaz

Faulknor

Berthiaume

et al. 2015

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

“…In some cases, for instance, the changes of the concentrations of both anions and cations were determined; from these data it seems that the electroneutrality was disturbed, which is obviously impossible. Moreover, Rabinowitch (110) has tried to check Freundlich's investigations, and has stated that the latter's methods were not quite correct. He therefore believes that the negative conclusions of Freundlich, Joachimson, and Ettisch are not sufficiently justified, and thinks that Freundlich's original adsorption theory of the flocculation may still be correct in some respects.…”

Section: Primary Stabilitymentioning

confidence: 99%