Simultaneous removal of aromatic pollutants and nitrate at high concentrations by hypersaline denitrification:Long-term continuous experiments investigation

Luo, Jing; Koju, Rashmi; Joshi, Tista Prasai; Liu, Ruiping; Liu, Huijuan; Qu, Jiuhui

doi:10.1016/j.watres.2022.118292

Cited by 20 publications

(6 citation statements)

References 63 publications

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“…Further 3D-EEM characterization showed that the three reactors exhibited similar organic composition in effluents, which was mainly composed of tryptophan protein (Peak A) and aromatic proteins (Peak B) (Figure S5). 39 Also, they showed a similar molecular weight distribution (Figure S6). Organic matter removal might be influenced by physical size rather than by chemical species or molecular weights.…”

Section: ■ Materials and Methodsmentioning

confidence: 72%

Performance and Mechanism of Improved Soluble Organic Carbon Recovery from Municipal Wastewater through the Coagulation-Enhanced High-Rate Contact Stabilization Process

Wang

Ren

et al. 2023

ACS EST Eng.

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High-rate contact stabilization is promising to capture organic carbon from municipal wastewater; however, its engineering application is restricted due to its poor capability of removing soluble organic matter (particle size < 0.1 μm). In this work, the carbon capture efficiency and robustness of the coagulation-enhanced high-rate contactstabilized process for treating municipal wastewater with a varied content of soluble organic matter was comprehensively evaluated. Results showed that the high-rate contact stabilization process did not perform well in the removal and capture of influent organic substances with a high proportion of soluble components. Adding coagulants into the above process favored organic carbon capture, whose recovery efficiency could reach up to 60% even under the 90% influent soluble chemical oxygen demand condition. Mechanism analysis revealed that the hydrolyzed product of the coagulant would adsorb dissolved components to form colloid and subcolloid particles, which was favored for further capture by sludge. The fitting result of the neutral community model indicated that both deterministic environmental factors and random processes influence the succession of sludge community. This work implied that the coagulation-enhanced high-rate contact stabilization process is feasible to recover organic carbon from municipal wastewater, which may shed a promising light for sustainable carbon control and management.

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Section: ■ Materials and Methodsmentioning

confidence: 72%

Performance and Mechanism of Improved Soluble Organic Carbon Recovery from Municipal Wastewater through the Coagulation-Enhanced High-Rate Contact Stabilization Process

Wang

Ren

et al. 2023

ACS EST Eng.

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“…Large quantities of macromolecules such as proteins, nucleic acids, phospholipids, and polysaccharides are synthesized during bacterial growth . Some of these biomolecules are secreted to the culture medium. , When exposed to nanomaterials (e.g., ZnO nanoparticles, TiO 2 nanoparticles, and graphene oxide), bacterial cells have been shown to produce additional proteins as a defensive strategy, essentially by mitigating external stress and promoting cell growth . Membrane transport is one of the main strategies for E.…”

Section: Resultsmentioning

confidence: 99%

“…43 Some of these biomolecules are secreted to the culture medium. 43,68 When exposed to nanomaterials (e.g., ZnO nanoparticles, 69 TiO 2 nanoparticles, 70 and graphene oxide 43 ), bacterial cells have been shown to produce additional proteins as a defensive strategy, 71 essentially by mitigating external stress and promoting cell growth. 43 Membrane transport is one of the main strategies for E. coli to deliver proteins from inside to outside of the cells.…”

mentioning

confidence: 99%

Transcriptome Analysis Reveals the Growth Promotion Mechanism of Enteropathogenic Escherichia coli Induced by Black Phosphorus Nanosheets

et al. 2023

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With the extensive production and application of black phosphorus (BP) nanosheets, release to the environment is inevitable, which raises concerns about the fate and effects of this two-dimensional (2D) material on sensitive receptors such as environmental microbes. Although the bacterial toxicity of BP nanosheets has been demonstrated, whether the biological response differs in pathogenic and nonpathogenic strains of a microorganism is unknown. Here, enteropathogenic Escherichia coli (EPEC) and nonpathogenic Escherichia coli DH5α (E. coli DH5α), Escherichia coli k12 (E. coli k12), and Bacillus tropicus (B. tropicus) are used to comparatively study the microbial toxicity of BP nanosheets. Upon exposure to BP nanosheets across a range of doses from 10 to 100 μg mL −1 for 12 h, EPEC experienced enhanced growth and E. coli DH5α and E. coli k12 were not affected, whereas B. tropicus exhibited clear toxicity. By combining transcriptome sequencing, proteome analysis, and other sensitive biological techniques, the mechanism of BPinduced growth promotion for EPEC was uncovered. Briefly, BP nanosheets activate the antioxidation system to resist oxidative stress, promote protein synthesis and secretion to attenuate membrane damage, enhance the energy supply, and activate growth-related pathways. None of these impacts were evident with nonpathogenic strains. By describing the mechanism of strain-dependent microbial effects, this study not only highlights the potential risks of BP nanosheets to the environment and to human health but also calls attention to the importance of model strain selection when evaluating the hazard and toxicity of emerging nanomaterials.

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“…wastewater treatment [6] and resources recovery [7]. Therefore, various approaches have been proposed to regulate denitrification, including adding conductive materials [8], applying magnetic field [9], appropriate bioreactor designing and operations [10], etc. Among these strategies, optical technology is promising for its superior advantages, including low costs, environment-friendly, solar-to-chemical capability, selectivity and precise microbial control [11][12][13].This is because light can serve as signals of optogenetic switches to modulate cellular activities like light-sensitive promoters, ion channels, pumps and protein conformation variations [14].…”

Section: Introductionmentioning

confidence: 99%

From mechanism to application: decrypting light-regulated denitrifying microbiome through geometric deep learning

Liao

Bian

Zhao

et al. 2023

Preprint

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Background: Regulation on denitrifying microbiomes is crucial for sustainable industrial biotechnology and ecological nitrogen cycling. The holisticgenetic profiles of microbiomes can be provided by meta-omics. However, precise decryption and further applications of highly complex microbiomes and corresponding meta-omics datasets remain great challenges. Results: Here, we combined optogenetics and geometric deep learning, following the discover-model-learn-advance (DMLA) cycle, that successfully decrypted light-regulated denitrifying microbiomes and validated the model predictions in the wet lab. Graph neural networks (GNNs) exhibited superior performance in integrating gene expression and subcellular information to identify co-expressed gene panels. Enrichment analysis on critical gene panels successfully predicted the co-expression between reactive oxygen species (ROS) and nitrogen metabolism, as well as the divergent secretion system. Yellow light centralized metabolism fluxes to synthesize protein and increased the extracellular protein concentrations by 83.8%. Contrariwise, blue light decentralized the metabolism fluxes to secrete bioactive substances like secondary metabolites, cofactors and vitamins. The topological network of gene panels guided the scientific discovery of nitrate-superoxide co-regulation and biotechnology development that utilize superoxide to facilitate nitrate removal and realized 99.9% enhancement. Conclusions: Overall, our study showcased the potential of GNNs-empowered optogenetic approaches for regulating denitrification and accelerating the mechanistic discovery of microbiomes for in-depth research and versatile applications.

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Simultaneous removal of aromatic pollutants and nitrate at high concentrations by hypersaline denitrification:Long-term continuous experiments investigation

Cited by 20 publications

References 63 publications

Performance and Mechanism of Improved Soluble Organic Carbon Recovery from Municipal Wastewater through the Coagulation-Enhanced High-Rate Contact Stabilization Process

Performance and Mechanism of Improved Soluble Organic Carbon Recovery from Municipal Wastewater through the Coagulation-Enhanced High-Rate Contact Stabilization Process

Transcriptome Analysis Reveals the Growth Promotion Mechanism of Enteropathogenic Escherichia coli Induced by Black Phosphorus Nanosheets

From mechanism to application: decrypting light-regulated denitrifying microbiome through geometric deep learning

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