Simultaneous regeneration of articular cartilage and subchondral bone induced by spatially presented TGF-beta and BMP-4 in a bilayer affinity binding system

Re'em, Tali Tavor; Witte, Frank; Willbold, Elmar; Ruvinov, Emil; Cohen, Smadar

doi:10.1016/j.actbio.2012.05.014

Cited by 108 publications

(86 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, endochondral ossification occurred exclusively in the MSC layer, which showed signs of mineralization following culture in hypertrophic medium containing β-glycerophosphate, as well as after subcutaneous implantation at a non-load bearing site in nude mice. 77 Another approach entails presenting growth factors in a biomimetic fashion, as seen in work done by Re'em and colleagues, which involved affinity-binding of TGF-β1 and BMP-4 to separate portions of a bilayered alginate hydrogel via alginate-sulfate 69 . Consequently, hMSCs responded to the prolonged expression of these growth factors and could more effectively differentiate into the desired chondrogenic and osteogenic phenotypes than in scaffolds containing unbound counterparts.…”

Section: Scaffoldsmentioning

confidence: 99%

Biomaterials for Tissue Engineering

Birla

2014

Introduction to Tissue Engineering

View full text Add to dashboard Cite

Biomaterials serve as an integral component of tissue engineering. They are designed to provide architectural framework reminiscent of native extracellular matrix in order to encourage cell growth and eventual tissue regeneration. Bone and cartilage represent two distinct tissues with varying compositional and mechanical properties. Despite these differences, both meet at the osteochondral interface. This article presents an overview of current biomaterials employed in bone and cartilage applications, discusses some design considerations, and alludes to future prospects within this field of research.

show abstract

Section: Scaffoldsmentioning

confidence: 99%

Biomaterials for Tissue Engineering

Birla

2014

Introduction to Tissue Engineering

View full text Add to dashboard Cite

show abstract

“…Dormer et al 175 designed PLGA microsphere-sintered constructs incorporated with gradients of BMP-2 and TGF-b1, and despite obtaining promising data, acknowledged the difficulty in sustaining the gradient patterns for long term in vitro due to the quick diffusion of the molecules. Re'em et al 176 designed a bilayered system, spatially presenting the chondroinductive TGF-b1 in one layer and the osteoinductive BMP-4 in a second layer via affinity binding to the matrix. When implanted in subchondral defects in rabbits, the constructs were able to induce the migration of host stem cells that sensed the biological cues spatially presented in the hydrogel and responded by differentiating into the appropriate cell lineage.…”

Section: From Single To Multiple Bioactive Factor Delivery For Skeletmentioning

confidence: 99%

Controlled Release Strategies for Bone, Cartilage, and Osteochondral Engineering—Part II: Challenges on the Evolution from Single to Multiple Bioactive Factor Delivery

Santo

Gomes²,

Mano³

et al. 2013

Tissue Engineering Part B: Reviews

102

View full text Add to dashboard Cite

The development of controlled release systems for the regeneration of bone, cartilage, and osteochondral interface is one of the hot topics in the field of tissue engineering and regenerative medicine. However, the majority of the developed systems consider only the release of a single growth factor, which is a limiting step for the success of the therapy. More recent studies have been focused on the design and tailoring of appropriate combinations of bioactive factors to match the desired goals regarding tissue regeneration. In fact, considering the complexity of extracellular matrix and the diversity of growth factors and cytokines involved in each biological response, it is expected that an appropriate combination of bioactive factors could lead to more successful outcomes in tissue regeneration. In this review, the evolution on the development of dual and multiple bioactive factor release systems for bone, cartilage, and osteochondral interface is overviewed, specifically the relevance of parameters such as dosage and spatiotemporal distribution of bioactive factors. A comprehensive collection of studies focused on the delivery of bioactive factors is also presented while highlighting the increasing impact of platelet-rich plasma as an autologous source of multiple growth factors.

show abstract

“…The goal of these strategies is to use cells, scaffolds and growth factors to regenerate the damaged tissue and thus provide long term relief to the patient (2). While numerous advances have been made in the design of multiphasic scaffolds (3)(4)(5), cell culture protocols (6,7) and growth factor releasing materials (8,9), a number of issues still exist which limit the widespread implementation of these techniques. One of the most prominent of these is promoting the formation of stable bone and cartilage within the osseous and chondral regions of the defect respectively.…”

Section: Introductionmentioning

confidence: 99%

A Computational Model of Osteochondral Defect Repair Following Implantation of Stem Cell-Laden Multiphase Scaffolds

O'Reilly

Kelly

2017

Tissue Engineering Part A

View full text Add to dashboard Cite

Tissue engineering (TE) has recently emerged as a potential method with which to treat osteochondral defects. In order to improve these strategies, an understanding is required of how the cells within an implanted scaffold interact with their local environment.To this end, the objective of this study was to develop a computational model of an osteochondral defect following the implantation of a tissue engineered scaffold. In particular, the purpose of this was to systematically explore the relationship that scaffold design has on the spatial formation of cartilage and bone within the defect. In this study, tissue formation was predicted using a previously developed algorithm in which cell fate was dependent upon the local oxygen tension and the local mechanical environment. In the first part of this study, the model was updated to include a condition whereby mature cartilage was resistant to both terminal differentiation and vascularisation. The updated model was then tested by using it to predict tissue formation during the spontaneous repair process within an osteochondral defect. Following this, the model was used to simulate an osteochondral defect treated with different variations of a previously developed multiphasic scaffold. By comparing the results of the different simulations it was possible to elucidate a mechanism by which the scaffold promoted robust bone and cartilage formation. In particular, support was provided for a previous hypothesis which proposed that osteochondral defect repair can be enhanced by actively confining angiogenesis to within the subchondral region of the defect for a sustained period of time. Based on the results of this study, it was possible to expand on this by suggesting that this time period be no less than 10 weeks. It is possible to reduce this, however, by pre-culturing MSCs under chondrogenic conditions prior to implantation within the defect.

show abstract

Simultaneous regeneration of articular cartilage and subchondral bone induced by spatially presented TGF-beta and BMP-4 in a bilayer affinity binding system

Cited by 108 publications

References 34 publications

Biomaterials for Tissue Engineering

Biomaterials for Tissue Engineering

Controlled Release Strategies for Bone, Cartilage, and Osteochondral Engineering—Part II: Challenges on the Evolution from Single to Multiple Bioactive Factor Delivery

A Computational Model of Osteochondral Defect Repair Following Implantation of Stem Cell-Laden Multiphase Scaffolds

Contact Info

Product

Resources

About