Simultaneous recognition of ionocytes and mucous cells in the gill epithelium of turbot and in the rat stomach

Watrin, Annette; Mayer-Gostan, N.

doi:10.1002/(sici)1097-010x(19961001)276:2<95::aid-jez2>3.0.co;2-8

Cited by 14 publications

(13 citation statements)

References 16 publications

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“…Carbonic anhydrase was detected using a commercial rabbit antibovine erythrocyte CA polyclonal antibody (CA IIb) (Biogenesis, Poole, UK). This antibody has been used successfully in teleost fishes (Watrin and Mayer-Gostan, 1996).…”

Section: Antibodiesmentioning

confidence: 99%

Ionoregulatory changes during metamorphosis and salinity exposure of juvenile sea lamprey (Petromyzon marinusL.)

Patrick¹,

McCormick

Wilson³

2008

Journal of Experimental Biology

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SUMMARY Ammocoetes of the anadromous sea lamprey Petromyzon marinus L. spend many years in freshwater before metamorphosing and migrating to sea. Metamorphosis involves the radical transformation from a substrate-dwelling,filter feeder into a free-swimming, parasitic feeder. In the present work we examined osmoregulatory differences between ammocoetes and transformers(metamorphic juveniles), and the effects of salinity acclimation. We measured the expression of key ion-transporting proteins[Na+/K+-ATPase, vacuolar (V)-type H+-ATPase and carbonic anhydrase (CA)] as well as a number of relevant blood parameters(hematocrit, [Na+] and [Cl–]). In addition,immunofluorescence microscopy was used to identify and characterize the distributions of Na+/K+-ATPase, V-type H+-ATPase and CA immunoreactive cells in the gill. Ammocoetes did not survive in the experiments with salinities greater than 10‰,whereas survival in high salinity (⩾25–35‰) increased with increased degree of metamorphosis in transformers. Plasma [Na+] and[Cl–] of ammocoetes in freshwater was lower than transformers and increased markedly at 10‰. In transformers, plasma ions increased only at high salinity (>25‰). Branchial Na+/K+-ATPase levels were ∼ tenfold higher in transformers compared to ammocoetes and salinity did not affect expression in either group. However, branchial H+-ATPase expression showed a negative correlation with salinity in both groups. Na+/K+-ATPase immunoreactivity was strongest in transformers and associated with clusters of cells in the interlamellar spaces. H+-ATPase (B subunit) immunoreactivity was localized to epithelial cells not expressing high Na+/K+-ATPase immunoreactivity and having a similar tissue distribution as carbonic anhydrase. The results indicate that branchial Na+/K+-ATPase and salinity tolerance increase in metamorphosing lampreys, and that branchial H+-ATPase is downregulated by salinity.

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Section: Antibodiesmentioning

confidence: 99%

Ionoregulatory changes during metamorphosis and salinity exposure of juvenile sea lamprey (Petromyzon marinusL.)

Patrick¹,

McCormick

Wilson³

2008

Journal of Experimental Biology

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“…Metals affect not only the fish morphology, but also all biological activities [12]. Among the heavy metals; Cd and Pb are the serious heavy metals that produce histopathological alterations including liver damage [13], respiratory dysfunctions [14], testicular and ovarian alterations [15][16][17]. These pathological alterations were primarily attributed to damage of cell membranes which allows higher uptake of Cd and Pb and thus the injury extends to more critical targets [18].…”

Section: Introductionmentioning

confidence: 99%

Impact of Environmental Contaminants on the Testes of Oreochromis niloticus with Special Reference to Ultrastructure of Spermatozoa in Lake Manzala (Egypt)

Shalaby¹,

Migeed²

2012

J Environ Anal Toxicol

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IntroductionThe importance of Nile tilapia, Oreochromis niloticus in aqua cultures is due to its spreading in most countries of the world [1]. It breads in captivity and wide variety of water conditions [2]. Lake Manzalah (LM) is one of the most important lakes in Egypt that provides more than 70% of the total fishery of the country [3] and considered as a highly polluted with toxic heavy metals and pesticides due to progressive increase of industrial and agricultural drainage, as well as sewage out fall from the surrounding governorates [4]. The mean values of some heavy metals (Cd, Pb, Fe, Cr and Mn) recorded in LM were higher than the world permissible levels [5] and according to the Egyptian laws [6]. Such heavy metals are not lethal to fish, but concentrated in their flesh and create a hazard to the consumer [7,8]. Cd, Pb, Zn, and Cr are considered the main causes of pollution in aquatic ecosystem [9]. The serious effect of these metals is derived from their persistence, toxicity and bioaccumulation, and consequently exert dangerous problem to man [10]. Experimental studies on heavy metals proved that they could impair the respiratory functions of the gills; consequently death in fish is probably caused by tissue hypoxia [11]. Metals affect not only the fish morphology, but also all biological activities [12]. Among the heavy metals; Cd and Pb are the serious heavy metals that produce histopathological alterations including liver damage [13], respiratory dysfunctions [14], testicular and ovarian alterations [15][16][17]. These pathological alterations were primarily attributed to damage of cell membranes which allows higher uptake of Cd and Pb and thus the injury extends to more critical targets [18]. Many studies focused on bioaccumulation of heavy metals in fish and biological studies. Thus we aimed in this work to study the testicular histopathological alterations of Oreochromis niloticus using light microscope and the mature sperm using TEM. Materials and Methods Samples collectionSamples of water and 48 specimen of Nile tilapia, Oreochromis niloticus about 14-20 cm in length and 120-200 g in weight were collected from two areas; Demitta branch of RN at Mansoura city as a control site and LM as a polluted area during the spawning season from March-June and transported to the laboratory in tanks provided with oxygen pump. The fish were weighed, dissected and the testes were Abstract Background: Pollution of the aquatic environment by inorganic and organic chemicals is a major factor posing serious threat to the survival of aquatic organisms including fish. Lake Manzala (LM) is one of the Egypt's northern Delta Lakes situated on the Mediterranean Coast of the Delta. It is affected by drainage water polluted by different heavy metals that their concentrations exceed the maximum world permissible levels.

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“…Zinc iodide/osmium (ZIO) fixation is known to colour ionocytes (also called chloride cells) in fish gill (Avella et al 1987;Mayer-Gostan et al 1987;Madsen 1990;Watrin and Mayer-Gostan 1996) and has thus been used to detect the presence of cells that might react in the same manner as gill ionocytes. The saccular tissue has also been incubated with the isomeric styryl fluorescent dye dimethylaminostyrylmethylpyridiniumiodine (DASPMI;Bereiter-Hahn 1976;Mayer-Gostan et al 1987) in order to visualize mitochondria-rich cells.…”

Section: Introductionmentioning

confidence: 99%

Distribution of ionocytes in the saccular epithelium of the inner ear of two teleosts ( Oncorhynchus mykiss and Scophthalmus maximus)

Mayer-Gostan

Kossmann

Watrin

et al. 1997

Cell and Tissue Research

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The saccular membranes of trout (Oncorhynchus mykiss) and turbot (Scophthalmus maximus) were examined to characterize specialized epithelial cells that might be responsible for ion exchange. The approach for localizing cell types was new for this tissue, as observations were made with a stereomicroscope and a light microscope in order to have a general view of the epithelium. No important differences between the two species were seen. The saccular tissue is a monolayer epithelium (except for the macula neural zone) surrounded by a layer of connective tissue invaded by many blood vessels. The use of the fluorescent probe DAPSMI and zinc iodide/osmium fixation-coloration defined two areas in which ionocytes were present. In the first, large ionocytes were grouped into a nearly complete, crowned meshwork around, but separated from, the macula. In the second area, opposite the macula, the ionocytes were smaller, cubical, and grouped in patches. Cells rich in Na+, K+-ATPase and carbonic anhydrase II were present in both areas. Contrary to previous studies in mammals and fish, ionocytes were also found in the epithelium of the saccule.

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Simultaneous recognition of ionocytes and mucous cells in the gill epithelium of turbot and in the rat stomach

Cited by 14 publications

References 16 publications

Ionoregulatory changes during metamorphosis and salinity exposure of juvenile sea lamprey (Petromyzon marinusL.)

Ionoregulatory changes during metamorphosis and salinity exposure of juvenile sea lamprey (Petromyzon marinusL.)

Impact of Environmental Contaminants on the Testes of Oreochromis niloticus with Special Reference to Ultrastructure of Spermatozoa in Lake Manzala (Egypt)

Distribution of ionocytes in the saccular epithelium of the inner ear of two teleosts ( Oncorhynchus mykiss and Scophthalmus maximus)

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