Simultaneous radioimmunoassay of plasma aldosterone, corticosterone, 11-deoxycorticosterone, progesterone, 17-hydroxyprogesterone, 11-deoxycortisol, cortisol and cortisone

Sippell, Wolfgang G.; Bidlingmaier, F.; Becker, H. M.; Brünig, T; Dörr, H. G.; Hahn, Hans Jürgen; Golder, W.; Hollmann, G.; Knorr, Dietrich

doi:10.1016/0022-4731(78)90104-8

Cited by 197 publications

(54 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Plasma steroids were measured using a method for the simultaneous determination of multiple adrenal steroids in a small plasma volume (1-2 ml), which was developed in our laboratory (16). The results are expressed in nmol/l; to convert to ng/ml divide by the following factors: aldosterone, 2.774; 18-OH-corticosterone, 2.759; corticosterone, 2.886; 18-OH-11-deoxycorticosterone, 2.886; 11-deoxycorticosterone, 3.026.…”

Section: Methodsmentioning

confidence: 99%

Molecular genetic study in two patients with congenital hypoaldosteronism (types I and II) in relation to previously published hormonal studies

Peter

Bünger

Drop

et al. 1998

European Journal of Endocrinology

View full text Add to dashboard Cite

We performed a molecular genetic study in two patients with congenital hypoaldosteronism. An original study of these patients was published in this Journal in 1982. Both index cases, a girl (patient 1) and a boy (patient 2), presented with salt-wasting and failure to thrive in the neonatal period. Parents of patient 1 were not related, whereas the parents of patient 2 were cousins. Endocrine studies had shown a defect in 18-oxidation of 18-OH-corticosterone in patient 1 and a defect in the 18-hydroxylation of corticosterone in patient 2. Plasma aldosterone was decreased in both patients, whereas 18-OH-corticosterone was elevated in patient 1 and decreased in patient 2. Plasma corticosterone and 11-deoxycorticosterone were elevated in both patients, whereas cortisol and its precursors were in the normal range. According to the nomenclature proposed by Ulick, the defects are termed corticosterone methyl oxidase (CMO) deficiency type II in patient 1, and type I in patient 2 respectively. Genetic defects in the gene CYP11B2 encoding aldosterone synthase have been described in a few cases. In patient 1, we identified only one heterozygous amino acid substitution (V386A) in exon 7, which has no deleterious effect on the enzyme activity. In patient 2 and his older brother, we identified a homozygous single base exchange (G to T) in codon 255 (GAG), causing a premature stop codon E255X (TAG). The mutant enzyme has lost the five terminal exons containing the haem binding site, and is thus a loss of function enzyme. This is only the second report of a patient with CMO deficiency type II without a mutation in the exons and exon-intron boundaries, whereas the biochemical phenotype of the two brothers with CMO deficiency type I can be explained by the patient's genotype.

show abstract

Section: Methodsmentioning

confidence: 99%

Molecular genetic study in two patients with congenital hypoaldosteronism (types I and II) in relation to previously published hormonal studies

Peter

Bünger

Drop

et al. 1998

European Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…Levels of unconjugated aldosterone, corticosterone, 1 1 -deoxycorticosterone, progesterone, 17-hydroxyprogesterone, I 1-deoxycortisol, cortisol, and cortisone were measured from the same small plasma sample. The micromethod of multisteroid analysis has been previously described and evaluated (9,10). In brief, it included the addition of tracer amounts of each of the eight steroids to a 250-p1 plasma sample, extraction with methylene chloride, and a mechanized Sephadex LH-20 chromatography for the simultaneous separation of the steroids.…”

Section: Doerrmentioning

confidence: 99%

Adrenocortical Steroids in Small-for-Gestational-Age Term Infants during the Early Neonatal Period1,2

et al. 1989

View full text Add to dashboard Cite

ABSTRACT. We evaluated adrenocortical steroid concentrations a t birth and during postnatal adaptation (2 h until 7 days) in 10 vaginally delivered term small-for-gestational-age (SGA) infants and 1 2 term appropriate-forgestational age infants. Plasma aldosterone, ll-deoxycorticosterone, corticosterone, progesterone, 17-hydroxyprogesterone, 11-deoxycortisol, cortisol, and cortisone were longitudinally measured by specific RIA after Sephadex LH-20 chromatography. Mean aldosterone was significantly higher in SGA than in appropriate-for-gestationalage infants (2 h to 7 days; p < 0.001). In SGA infants, cortisone and cortisol levels were significantly lower in umbilical artery (p < 0.05), and all glucocorticoid levels were significantly lower 12 h after birth (p < 0.05). Thereafter (24 h to 7 days), only 11-deoxycortisol levels remained significantly lower in SGA; corticosterone and cortisol levels were even higher @ < 0.05) in SGA 24 h after birth. The data suggest that SGA infants maintain high aldosterone levels throughout the 1st wk of life. Low cortisol and cortisone levels in umbilical artery a s well a s low glucocorticoid levels a t 2 h and/or 1 2 h compared to term appropriate-for-gestational-age infants may reflect either a less stressful postnatal adaptation or, more likely, a reduced adrenocortical synthesis in term SGA infants. (Pediatr Res 25:115-118, 1989) Abbreviations SGA, small for gestational age UA, umbilical artery AGA, appropriate for gestational age Perinatal problems in neonates with severe intrauterine growth retardation are frequent. They include asphyxia, a high neonatal mortality, electrolyte imbalance, hypoglycemia, cardiorespiratory problems, and neurologic abnormalities (1). Organ weights are significantly reduced (2); in particular, the adrenal cortex is small with respect to size and cell number (3).

show abstract

“…For full-term healthy infants, data on plasma steroid levels in the neonatal period are available (2,3), but studies on adrenal steroids in healthy premature infants are still lacking. Our micromethod of multisteroid analysis (4,5) enabled us to measure simultaneously in a single small plasma sample individual mineralocorticoids, glucocorticoids, and progestins in premature infants at birth and longitudinally during the first hours and days of life.…”

Section: P Progesterone 170hp 17-hydroxyprogesteronementioning

confidence: 99%

“…The micromethod of multisteroid analysis has been previously described and evaluated (4,5). In brief, it included the addition of tracer amounts of each of the eight steroids to a 250-p1 plasma sample, extraction with methylene chloride, and a mechanized Sephadex LH-20 chromatography for the simultaneous separation of the steroids.…”

Section: Methodsmentioning

confidence: 99%

Plasma Mineralocorticoids, Glucocorticoids, and Progestins in Premature Infants: Longitudinal Study during the First Week of Life

et al. 1988

View full text Add to dashboard Cite

ABSTRACT. Plasma levels of aldosterone, corticosterone, 11-deoxycorticosterone, progesterone, 17-hydroxyproge.sterone, 11-deoxycortisol, cortisol, and cortisone were measured simultaneously by a micromethod of multisteroid analysis in eight vaginally delivered premature infants (PI) of 33-36 wk gestation with uneventful peri-and postnatal course. Mean concentrations (nglml) in umbilical arterial and in peripheral venous or capillary plasma sampled longitudinally at age 2 h to 7 days were compared with the same kind of data obtained from a group of 12 term infants (TI) who served as controls. Mean aldosterone was two to five times higher in PI than in TI (umbilical artery, 2 h to 7 days; p < 0.05), whereas 11-deoxycorticosterone was lower in PI from 2 h ( p < 0.01) until 7 days (NS).Corticosterone was significantly higher in PI than TI at 6 and 24 h after birth, whereas cortisol was slightly lower (NS) in PI in umbilical artery and 2 h after birth, but higher (p c 0.02) at 6 h, showing less variation in PI than in TI. 17-Hydroxyprogesterone levels in PI were two to three times higher ( p c 0.02) during 6 h until 7 days after birth. The data suggest that PI are able to maintain high aldosterone levels in the early neonatal period. Higher levels of the active glucocorticoids (cortisol and corticosterone) seen after delivery point to a more stressful extrauterine adaptation of PI. Furthermore, the data demonstrate that the adrenal cortex is fully functioning in premature infants (33-36 wk gestation) as well as in term infants. (Pediatr Res 23: 525-529,1988) Abbreviations RDS, respiratory distress syndrome UA, umbilical artery Aldo, aldosterone DOC, 11-deoxycorticosterone E, cortisone S, 11-deoxycortisol B, corticosterone F, cortisol P. progesterone 170HP, 17-hydroxyprogesterone Abrupt cessation of steroid supply from the placenta after severing the umbilical cord, and rapid postnatal involution of the fetal zone of the adrenal cortex dramatically changes the hormonal milieu of the fetus after birth. There is increasing evidence that the adrenal cortex plays an important role in the postnatal adaptation of newborns (1). For full-term healthy infants, data on plasma steroid levels in the neonatal period are available (2, 3), but studies on adrenal steroids in healthy premature infants are still lacking. Our micromethod of multisteroid analysis (4, 5) enabled us to measure simultaneously in a single small plasma sample individual mineralocorticoids, glucocorticoids, and progestins in premature infants at birth and longitudinally during the first hours and days of life.Herein we assess in detail the adrenocortical function of healthy moderately premature infants after birth and provide reference data for the physiologically most important corticosteroids. MATERIALS AND METHODSEight premature appropriate for gestational age infants (33-36 wk gestation) of both sexes (three males, five females) with uneventful peri-and postnatal courses were studied longitudinally from birth to day 7. Their mothers (20-36 yr) were heal...

show abstract

Simultaneous radioimmunoassay of plasma aldosterone, corticosterone, 11-deoxycorticosterone, progesterone, 17-hydroxyprogesterone, 11-deoxycortisol, cortisol and cortisone

Cited by 197 publications

References 40 publications

Molecular genetic study in two patients with congenital hypoaldosteronism (types I and II) in relation to previously published hormonal studies

Molecular genetic study in two patients with congenital hypoaldosteronism (types I and II) in relation to previously published hormonal studies

Adrenocortical Steroids in Small-for-Gestational-Age Term Infants during the Early Neonatal Period1,2

Plasma Mineralocorticoids, Glucocorticoids, and Progestins in Premature Infants: Longitudinal Study during the First Week of Life

Contact Info

Product

Resources

About