Simultaneous quantitative screening of 53 phytochemicals in 33 species of medicinal and aromatic plants: A detailed, robust and comprehensive LC–MS/MS method validation

Yılmaz, Mustafa Abdullah

doi:10.1016/j.indcrop.2020.112347

Cited by 111 publications

(74 citation statements)

References 32 publications

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“…Binary pumps (LC 30AD), a column oven (CTO 10 ASvp), an autosampler (SIL 30 AC) and a degasser (DGU 20 A3R) were the components of the separation system (the chromatograph). The analyses were performed by a previously developed and validated LC/MS/MS method [73] . The optimized chromatographic conditions were as follows: Agilent‐Poroshell 120 EC−C18 (150 mm×2.1 mm, 2.7 μm) reversed phase analytical column was used, LC column temperature was at 40 °C, eluent A (water/5 mM ammonium formate/0.1 % formic acid) and eluent B (methanol/5 mM ammonium formate/0.1 % formic acid) were the components of gradient elution.…”

Section: Methodsmentioning

confidence: 99%

“…The MRM method was optimized to selectively detect and quantify phytochemical compounds based on the screening of specified precursor phytochemical‐to‐fragment ion transitions. Nebulizer gas (nitrogen) flow, drying gas (nitrogen) flow, heat block temperature, DL temperature, and interface temperature were optimized as 3 L/min, 15 L/min, 400 °C, 250 °C and 350 °C, respectively [73] . All analytical characteristics are given in the supplemental material (Table S1).…”

Section: Methodsmentioning

confidence: 99%

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Chemical Composition and Pharmacological Evaluation and of Toddalia asiatica (Rutaceae) Extracts and Essential Oil by in Vitro and in Silico Approaches.

Lobine

Pairyanen

Zengin

et al. 2021

Chemistry & Biodiversity

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Toddalia asiatica (L.) Lam. is extensively used in traditional medicinal systems by various cultures. Despite its frequent use in traditional medicine, there is still a paucity of scientific information on T. asiatica growing on the tropical island of Mauritius. Therefore, the present study was designed to appraise the pharmacological and phytochemical profile of extracts (methanol, ethyl acetate and water) and essential oil obtained from aerial parts of T. asiatica. Biological investigation involved the evaluation of in vitro antioxidant and enzyme inhibitory potentials. The chemical profile of the EO was determined using gas chromatography coupled to mass spectrometry (GC/MS) analysis, while for the extracts, the total phenolic (TPC) and flavonoid content were quantified as well as their individual phenolic compounds by LC/MS/MS. Quinic acid, fumaric acid, chlorogenic acid, quercitrin and isoquercitrin were the main compounds in the extracts. Highest total phenolic (82.5±0.94 mg gallic acid equivalent (GAE/g)) and flavonoid (43.8±0.31 mg rutin equivalent (RE/g)) content were observed for the methanol extract. The GC/MS analysis has shown the presence of 26 compounds with linalool (30.9 %), linalyl acetate (20.9 %) and β‐phellandrene (7.9 %) being most abundant components in the EO. The extracts and EO showed notable antioxidant properties, with the methanol extract proved to be superior source of antioxidant compounds. Noteworthy anti‐acetylcholinesterase (AChE) and anti‐butyrylcholinesterase (BChE) effects were recorded for the tested samples, while only the methanol and ethyl acetate extracts were active against tyrosinase. With respect to antidiabetic effects, the extracts and EO were potent inhibitors of α‐glucosidase, while modest activity was recorded against α‐amylase. Docking results showed that linalyl acetate has the highest affinity to interact with the active site of BChE with docking score of −6.25 kcal/mol. The findings amassed herein act as a stimulus for further investigations of this plant as a potential source of bioactive compounds which can be exploited as phyto‐therapeutics.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Chemical Composition and Pharmacological Evaluation and of Toddalia asiatica (Rutaceae) Extracts and Essential Oil by in Vitro and in Silico Approaches.

Lobine

Pairyanen

Zengin

et al. 2021

Chemistry & Biodiversity

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“…LC/MS/MS instrument (Shimadzu Nexera model UHPLC and Shimadzu LCMS 8040 model triple quadrupole mass spectrometer) was used to analyze phenolic and flavonoid compounds. Fifty‐three phytochemicals, including phenolic and flavonoid compounds, were screened in ethanol extracts of Gundelia species using a previously developed and validated LC/MS/ MS method [26,33] . The analytical separation was achieved on an Agilent Poroshell 120 EC‐C18 model (150 mm×2.1 mm, 2.7 μm) reverse phase column.…”

Section: Methodsmentioning

confidence: 99%

Phytochemical Fingerprints and Bioactivities of Ripe Disseminules (Fruit‐Seeds) of Seventeen Gundelia (Kenger‐Kereng Dikeni) Species from Anatolia with Chemometric Approach

Ertaş

Fırat

Yener

et al. 2021

Chemistry & Biodiversity

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Gundelia species are known as “Kenger‐kereng dikeni” in Anatolia, and their aerial parts are consumed as food. Also, roots and seeds (disseminules) of the Gundelia species are used to prepare gum and coffee. The chemical contents of ethanol and hexane extracts of disseminules of 17 Gundelia species, 13 of them are endemic, were studied using LC/MS/MS and GC/MS. Additionally, their antioxidant potential and enzyme inhibitory capacity against acetyl‐ and butyryl‐cholinesterase, urease, and tyrosinase were determined. The unsaturated fatty acid ratios of Gundelia species were higher than their saturated fatty acid ratio. The highest sum of oleic and linoleic acid was detected in G. tournefortii var. tenuisecta (70.42 %). β‐Sitosterol, α‐amyrin, 3‐acetyllupeol were identified in 17 Gundelia species by GC/MS, while chlorogenic acid and luteolin by LC/MS/MS as major compounds. The ethanol and hexane extracts of G. siirtica, G. rosea, and G. mesopotamica indicated good cholinesterase inhibitory activity. Among all species, ethanol extract of G. colemerikensis exhibited the best activity in ABTS (IC50: 32.30±0.98 μg/mL), DPPH (IC50: 59.91±0.89 μg/mL), and CUPRAC (A0.5: 57.41±1.03 μg/mL) assays. Ethanol extract of G. colemerikensis also displayed the highest inhibitory activity against butyrylcholinesterase (51.14±0.25 % at 200 μg/mL), urease (51.71±1.75 % at 200 μg/mL), and tyrosinase (39.50±0.85 % at 200 μg/mL) enzymes. According to the chemometric analysis of fatty acids, four groups were observed. Therefore, it is suggested that G. colemerikensis can be used in the pharmaceutical, food, and cosmetic industries due to its antioxidant and enzyme inhibition properties.

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“…Binary pumps (LC 30AD), a column oven (CTO 10 ASvp), an autosampler (SIL 30 AC) and a degasser (DGU 20 A3R) were the components of the separation system (the chromatography). The analyses were performed by a previously developed and validated LC-MS/MS method (Yilmaz, 2020). The optimized chromatographic conditions were as follows: Agilent-Poroshell 120 EC-C18 (150 mm × 2.1 mm, 2.7 µm) reversed-phase analytical column was used, LC column temperature was 40 • C, eluent A (water/5 mM ammonium formate/0.1% formic acid) and eluent B (methanol/5 mM ammonium formate/0.1% formic acid) were the components of gradient elution.…”

Section: Phytochemical Analysismentioning

confidence: 99%

Chemical characterization and insecticidal effect of Moringa oleifera L. seeds extracts on common bean weevil (Acanthoscelides obtectus Say)

Songwe¹,

Bekolo²,

Tueguem³

et al. 2022

NRFHH

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Some insects cause high losses of the common bean during storage, namely, Acanthoscelides obtectus Say. Chemical insecticides are commonly used to control insects, but their overuse adversely affects the environment and consumers' health. There is, therefore, the need for an alternative control method. This study was aimed to evaluate the chemical characterization and insecticidal property of Moringa oleifera seed extract on the developmental stages of A. obtectus in stored beans. Three types of M. oleifera seed extracts, namely methanol, ethanol and acetone extracts, were used at doses of 12.5, 25 and 50 µl/ml each. HPLC-MS was used to characterize these extracts. The toxicity of extracts against the adults, the number of eggs and the viability rate of laid or emerged eggs of A. obtectus were evaluated. The results show that M. oleifera seed extract is rich in Hesperidin, Quinic acid, gallic acid, protocatechuic acid, 4-OH benzoic acid, cynaroside, isoquercitrin, cosmosiin, quercitrin, luteolin, naringenin, salicylic acid, Apigenin and Fumaric acid. The acetone extract, from day 2, showed a 100% mortality rate in all doses compared to about only 14% in the control treatment. The number of eggs laid in control (236.67) was higher than the null (0) in acetone treatment at 50 µl/ml. M. oleifera seed extracts contain chemical molecules. They significantly reduced the damage caused by A. obtectus on stored P. vulgaris grains. Therefore, they can be used as an alternative to chemicals for the protection of stored foodstuffs.

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Simultaneous quantitative screening of 53 phytochemicals in 33 species of medicinal and aromatic plants: A detailed, robust and comprehensive LC–MS/MS method validation

Cited by 111 publications

References 32 publications

Chemical Composition and Pharmacological Evaluation and of Toddalia asiatica (Rutaceae) Extracts and Essential Oil by in Vitro and in Silico Approaches.

Chemical Composition and Pharmacological Evaluation and of Toddalia asiatica (Rutaceae) Extracts and Essential Oil by in Vitro and in Silico Approaches.

Phytochemical Fingerprints and Bioactivities of Ripe Disseminules (Fruit‐Seeds) of Seventeen Gundelia (Kenger‐Kereng Dikeni) Species from Anatolia with Chemometric Approach

Chemical characterization and insecticidal effect of <i>Moringa oleifera</i> L. seeds extracts on common bean weevil (<i>Acanthoscelides obtectus</i> Say)

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