Simultaneous Quantitative Determination of Metoprolol, Atorvastatin and Ramipril in Capsules by a Validated Stability-Indicating RP-UPLC Method

Abstract

A simple ultra performance liquid chromatographic (UPLC) method has been developed for the simultaneous estimation of Metoprolol (MT), Atorvastatin (AT) and Ramipril (RM) from capsule dosage form. The method was developed using Zorbax® XDB-C18 (4.6 mm × 50 mm, 1.8 μm) column with a mobile phase consisting of 0.06% ortho phosphoric acid in Milli Q® water having an ion pair reagent, 0.0045 M Sodium lauryl sulphate as buffer, at ratio of buffer: Acetonitrile (50:50 v/v), at 55°C column temperature with a flow rate of 1.0 ml/min. Detection was carried out with ultra-violet detection at 210 nm for RM, MT and AT respectively. The retention times were about 1.3, 2.1 and 2.6 min for MT, AT and RM respectively, the method was validated for linearity, accuracy, precision, specificity, robustness and ruggedness. The % mean recoveries are 101.9, 102.1 and 101.4 for MT, AT and RM respectively. The method was found to be rugged and robust and can be successfully used to determine the three drugs and its combinations.

An Improved Rapid HPLC Method for the Separation of Five Anti-Hypertensive Agents Using C18 Column: Application to Hydrochlorothiazide Determination in Bulk and Tablet Dosage Form

“…Literature survey reveals that for the determination of above said five anti-hypertensive agents in biological fluids like plasma, blood, urine and pharmaceutical dosage forms by spectrophotometry [1][2][3][4] , spectrofluorimetry [5] , TLC [6][7] , HPTLC [8] , High Performance Liquid Chromatography (RP-HPLC) with UV detection [9][10][11][12][13][14][15][16][17][18] , fluorimetric detection [19] , ion-pair HPLC [20] , RP-UPLC [21] and Liquid Chromatography-Mass Spectrometry [22][23] , Capillary electrophoresis [24] .In fact there is a need for the development of a novel, simple, rapid, efficient RP-HPLC analytical method with reproducibility for determination of HCT in bulk and pharmaceutical dosage forms. A group of five anti-hypertensive drugs investigated in the present study are listed in Figure 1.…”

A New Stability-Indicating Rp-HPLC-Pda Method for Simultaneous Estimation of Triplicate Mixture of Ramipril, Atorvastatin and Clopidogrel in Tablet Dosage Form

“…As per the literature survey several methods have been reported for the estimation of ramipril, atorvastatin, and clopidogrel individually or with the combination of some other drugs. Some of the methods were being; simultaneous estimation of atorvastatin, clopidogrel [21][22] ramipril, atorvastatin [23][24]. There is no official method for this combination so far as per our knowledge.…”

Isocratic Separation of Four Beta Blockers With Amlodipine by C18 Rp-Hplc: Application to Amlodipine Determination in Pharmaceutical Dosage Forms

“…NEBI is chemically1-(6-fluorochroman-2-yl)-{[2-(6-fluorochroman-2-yl)-2-hydroxy ethyl] amino} ethanol ( Figure 1e). Literature survey reveals that for the determination of above said five anti-hypertensive agents in biological fluids like plasma, blood, urine and pharmaceutical dosage forms by spectrophotometry [1][2][3][4] , spectrofluorimetry 5 , TLC [6][7] , HPTLC [8][9] , High Performance Liquid Chromatography (RP-HPLC) with UV detection [10][11][12][13][14][15][16][17][18][19][20][21] , fluorimetric detection 22 , ion-pair HPLC 23 , RP-UPLC 24 and Liquid Chromatography-Mass Spectrometry [25][26] . As a matter of fact there is a need for the development of a novel, simple, rapid, efficient RP-HPLC analytical method with reproducibility for determination of AMLO in bulk and pharmaceutical dosage forms.…”