Simultaneous quantitation of aconitine, mesaconitine, hypaconitine, benzoylaconine, benzoylmesaconine and benzoylhypaconine in human plasma by liquid chromatography–tandem mass spectrometry and pharmacokinetics evaluation of “SHEN-FU” injectable powder

Biological activities and pharmacokinetics of aconitine, benzoylaconine, and aconine after oral administration in rats

“…Some researchers have reported that Aconitum alkaloids were absorbed very quickly while the bioavailability was very low. [12,[18][19][20] Although many pharmacological studies on Aconitum alkaloids have been reported, [21][22][23][24][25] there are few systematic studies available on the pharmacokinetic characteristics and disposal process of the three forms of Aconitum alkaloids. The aim of this study was to determine the biological activities of three forms of Aconitum alkaloids in rat plasma, urine, faeces, and different tissues after oral administration by liquid chromatographytandem mass spectrometry (LC-MS/MS), investigate their disposal process in vivo, and clarify their pharmacokinetic parameters, absorption and metabolic stability by using a Caco-2 cell monolayer model and rat liver microsome incubation system in vitro, hoping that this study would provide references for rational clinical use of the Aconitum tuber, and for further research on the pharmacology or toxicology of this medicinal plant.…”

Simultaneous analysis of aconitine, mesaconitine, hypaconitine, and jesaconitine in whole blood by LC-MS-MS using a new polymer column

“…Only a few articles [8,11,12] have reported the simultaneous analysis of aconitine together with its analogues by LC-MS-MS. To our knowledge, this is the fi rst report dealing with LC-MS-MS analysis of mesaconitine, hypaconitine, and jesaconitine in whole blood. These aconitine analogues usually coexist in body fl uids and aconite herbal materials [5][6][7][8].…”

“…During the preparation of this manuscript, a similar report [12] appeared very recently; they dealt with simultaneous determination of aconitine, mesaconitine, hypaconitine, and their metabolites in plasma specimens by LC-MS-MS. Nevertheless, our report merits publication because of the added analysis of jesaconitine, and because the analysis with whole blood is essential for hemolyzed blood specimens in postmortem cases.…”

Relative Quantification of the Metabolite of Aconitine in Rat Urine by LC-ESI-MS/MS and Its Application to Pharmacokinetics

“…In addition, since aconitum alkaloids are very unstable and decomposed fast in the human body, 3 metabolite studies are helpful to understand the medication safety, toxification and detoxification mechanism. Earlier publications have described methods for aconitine determination in biological samples, [4][5][6][7][8][9][10] and the identification of metabolites. [11][12][13] This paper reports on a pharmacokinetics study of aconitine metabolite in rat urine after oral administration for the first time, using a simple and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method.…”