Simultaneous quantification of vasicine and vasicinone in different parts of Justicia adhatoda using high-performance thin-layer chromatography‒densitometry: comparison of different extraction techniques and solvent systems

Padhiari, Bhuban Mohan; Ray, Asit; Jena, Sudipta; Champati, Bibhuti Bhusan; Sahoo, Ambika; Mohanty, Swagat; Nayak, Sanghamitra

doi:10.1007/s00764-020-00070-9

Cited by 2 publications

(3 citation statements)

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“…Justicia adhatoda L. leaf Vasicine Vasicinone P = 1200 W, t = 6 min, sample/solvent = 0.025 g/ml, methanol (domestic microwave oven) [23].…”

Section: Paniculatementioning

confidence: 99%

“…Capital costs for closed vessel systems are high compared with traditional extraction methods. Applications for HPTLC analysis are limited so far to botanicals and are summarized in Table 3 [21–23, 45, 46]. In most cases, open vessels using domestic microwave ovens were used, which fail to take full advantage of the possibilities of MASE.…”

Section: Solvent Extraction (Solids)mentioning

confidence: 99%

“…Source Target Conditions compound (i) Ultrasound-assisted solvent extraction Tecomell undulate bark Stigmasterol T = 50 • C, t = 46 min, sample/solvent 52.8 mg/ml, n-hexane, yield = 2.45% (Soxhlet = 1.93% [38]. Sea red rice bran Ceremides T = 51 • C, t = 46 min, sample/solvent = 5 g/ml, 95% (v/v) ethanol, P = 360 W, f = 40 kHz [42].Ginkgo bilboa L. leaf Terpene lactones T = 70 • C, t = 30 min, sample/solvent 0.1 g/ml, methanol[41].Stevia rebaudiana leaf Stevioside T = 70 • C, t = 60 min, sample/solvent 0.33 g/ml, glycerol, particle size 0.075 mm, P = 200 W, f = 20 kHz, yield = 8.81 mg/g (Soxhlet = 11.12 mg/g)[27].Saffron Crocin T = r. t., t = 15 min, sample/solvent = 100 mg/ml, methanol-water (21:1), f = kHz[39].Magnolia bark Biphenyl T = r. t., t = 10 min, sample/solvent = 1 capsule/ml, neolignans methanol, P = 120 W, f = 40 kHz[23].Justicia adhatoda L. leaf Vasicine Vasicinone T = 30 • C, t = 60 min, sample/solvent = 0.025 g/ml, methanol, P = 120 W, f = 40 kHz[23]…”

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confidence: 99%

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Sample preparation for planar chromatography

Poole

2023

J of Separation Science

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High‐performance thin‐layer chromatography has favorable properties for high‐throughput separations with a high matrix tolerance. Sample preparation, however, is sometimes required to control specific matrix interferences and to enhance the detectability of target compounds. Trends in contemporary applications have shifted from absorbance and fluorescence detection to methods employing bioassays and mass spectrometry. Traditional methods (shake‐flask, heat at reflux, Soxhlet, and hydrodistillation) are being challenged by automated instrumental approaches (ultrasound‐assisted and microwave‐assisted solvent extraction, pressurized liquid extraction, and supercritical fluid extraction) and the quick, easy cheap, efficient, rugged, and safe extraction method for faster and streamlined sample processing. Liquid‐liquid extraction remains the most widely used approach for sample clean‐up with increasing competition from solid‐phase extraction. On‐layer sample, clean‐up by planar solid‐phase extraction is increasingly used for complex samples and in combination with heart‐cut multimodal systems. The automated spray‐on sample applicator, the elution head interface, biological detection of target and non‐target compounds, and straightforward mass spectrometric detection are highlighted as the main factors directing current interest toward faster and simpler sample workflows, analysis of more complex samples, and the determination of minor contaminants requiring high concentration factors.

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“…Justicia adhatoda L. leaf Vasicine Vasicinone P = 1200 W, t = 6 min, sample/solvent = 0.025 g/ml, methanol (domestic microwave oven) [23].…”

Section: Paniculatementioning

confidence: 99%

Section: Solvent Extraction (Solids)mentioning

confidence: 99%