Simultaneous quantification of the food allergens soy bean, celery, white mustard and brown mustard via combination of tetraplex real-time PCR and standard addition

Luber, Florian; Demmel, Anja; Pankofer, Katrin; Busch, Ulrich; Engel, Karl‐Heinz

doi:10.1016/j.foodcont.2014.06.047

Cited by 12 publications

(7 citation statements)

References 18 publications

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“…qPCR followed by HRM analysis is the most commonly used method for detection of celery [ 9 , 10 , 11 , 12 ]. In general, HRM analysis is performed on double-stranded PCR-amplified DNA samples by heating the DNA between 60 °C and 95 °C.…”

Section: Resultsmentioning

confidence: 99%

“…At present, available ELISAs for the specific detection of celery show cross-reactivity with closely related members of the Apiaceae family, such as carrots [ 2 , 6 , 7 ] or parsley [ 8 ]. Therefore, several real-time (i.e., quantitative) PCR (qPCR) assays have been developed as a more specific alternative [ 9 , 10 , 11 , 12 ], targeting sequences from the Api g 1 or the mannitol dehydrogenase ( Mtd ) gene. Although Api g 1 codes for the major allergenic protein of celery, Mtd is more widely recommended for use in these assays due to the lower risk of cross-reactivity with sequences of other (closely) related species [ 10 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Identification and Quantification of Celery Allergens Using Fiber Optic Surface Plasmon Resonance PCR

Daems

Peeters

Delport

et al. 2017

Sensors

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Accurate identification and quantification of allergens is key in healthcare, biotechnology and food quality and safety. Celery (Apium graveolens) is one of the most important elicitors of food allergic reactions in Europe. Currently, the golden standards to identify, quantify and discriminate celery in a biological sample are immunoassays and two-step molecular detection assays in which quantitative PCR (qPCR) is followed by a high-resolution melting analysis (HRM). In order to provide a DNA-based, rapid and simple detection method suitable for one-step quantification, a fiber optic PCR melting assay (FO-PCR-MA) was developed to determine different concentrations of celery DNA (1 pM–0.1 fM). The presented method is based on the hybridization and melting of DNA-coated gold nanoparticles to the FO sensor surface in the presence of the target gene (mannitol dehydrogenase, Mtd). The concept was not only able to reveal the presence of celery DNA, but also allowed for the cycle-to-cycle quantification of the target sequence through melting analysis. Furthermore, the developed bioassay was benchmarked against qPCR followed by HRM, showing excellent agreement (R2 = 0.96). In conclusion, this innovative and sensitive diagnostic test could further improve food quality control and thus have a large impact on allergen induced healthcare problems.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification and Quantification of Celery Allergens Using Fiber Optic Surface Plasmon Resonance PCR

Daems

Peeters

Delport

et al. 2017

Sensors

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“…The sample curve plotted was dependent on the initial amount of target in sample, which could be calculated when compared to the external standard curve of target. The ‘modified standard addition’ was also successfully used in quantitative tetraplex real-time PCR of soybean, celery, white mustard and brown mustard (Luber et al 2014b ). Costa et al ( 2012 ) successfully used RT-PCR to detect almond in a commercial food product and to distinguish almond from related species by amplifying a target gene encoding the Pru du 5 allergen in almond.…”

Section: Advances In Rt-pcr Applicationsmentioning

confidence: 99%

Trends and advances in food analysis by real-time polymerase chain reaction

et al. 2016

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Analyses to ensure food safety and quality are more relevant now because of rapid changes in the quantity, diversity and mobility of food. Food-contamination must be determined to maintain health and up-hold laws, as well as for ethical and cultural concerns. Real-time polymerase chain reaction (RT-PCR), a rapid and inexpensive quantitative method to detect the presence of targeted DNA-segments in samples, helps in determining both accidental and intentional adulterations of foods by biological contaminants. This review presents recent developments in theory, techniques, and applications of RT-PCR in food analyses, RT-PCR addresses the limitations of traditional food analyses in terms of sensitivity, range of analytes, multiplexing ability, cost, time, and pointof-care applications. A range of targets, including species of plants or animals which are used as food ingredients, food-borne bacteria or viruses, genetically modified organisms, and allergens, even in highly processed foods can be identified by RT-PCR, even at very low concentrations. Microfluidic RT-PCR eliminates the separate sample-processing step to create opportunities for point-of-care analyses. We also cover the challenges related to using RT-PCR for food analyses, such as the need to further improve sample handling.

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“…However, legislation does not consider the wider need for warnings with regard to traces of allergenic material that at some point in the food supply chain may cross-contaminate and form an inclusion in what otherwise is considered in the legislation context as a non-allergenic food product. This argument was first highlighted more than a decade ago by Anandan and Sheikh (2005) and Said and Weiner (2004) leading to food manufacturers using an "alibi" or precautionary allergen labelling (PAL) on their packaging via statements such as "may contain" where there is a potential for crosscontamination (Luber et al 2015). The aim of this paper is to review the development and use of PAL, then consider the proliferation of various types of PAL descriptors.…”

Section: Introductionmentioning

confidence: 99%

“May Contain” Allergen Statements: Facilitating or Frustrating Consumers?

Soon

Manning

2017

J Consum Policy

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As a result of mandatory labelling legislation, major food allergens that commonly cause allergic reactions are declared on packaging. The usage of precautionary allergen labelling (PAL) on packaging is not regulated in all countries, and the food industry uses various forms of "may contain" labelling which firstly is often inconsistent and secondly over time may diminish the value of such advisory statements. Hence, the aims of this paper are to review the current industry usage of PAL and to provide recommendations on future use that are of value to academics, policy makers, food industry, and consumers. A case study example is used to illustrate the likely costs and benefits of improving the current PAL status by considering a "peanut-free" product and calculation using the Voluntary Incidental Trace Allergen Labelling (VITAL) calculator. Governance such as addressing the inconsistent usage of PALs, promoting the harmonization of language used in PALs, and improving PAL status to quantified PAL statements would be helpful in communicating risks to consumers, so they can make informed choices when purchasing food products.

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Simultaneous quantification of the food allergens soy bean, celery, white mustard and brown mustard via combination of tetraplex real-time PCR and standard addition

Cited by 12 publications

References 18 publications

Identification and Quantification of Celery Allergens Using Fiber Optic Surface Plasmon Resonance PCR

Identification and Quantification of Celery Allergens Using Fiber Optic Surface Plasmon Resonance PCR

Trends and advances in food analysis by real-time polymerase chain reaction

“May Contain” Allergen Statements: Facilitating or Frustrating Consumers?

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