Simultaneous Quantification of Oxcarbazepine and Its Active Metabolite in Spiked Human Plasma Using Ultra Performance Liquid Chromatography–MS/MS

Rajendran, Karthik; Chekraverthy, Bhadram Kalyan; Devendran, Rajendran Sankham; Sajini, R Jasmin; Nagappan, Krishnaveni

doi:10.4155/bio-2021-0125

Cited by 3 publications

(1 citation statement)

References 30 publications

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“…The experimental protocol for the pharmacokinetic study was carried out according to our previous publication [28]. In brief, six healthy human adult male volunteers were administrated with a single dosage of 25 mg/5 mL spironolactone oral suspension, either test product or reference product, through a 5‐day washout period.…”

Section: Methodsmentioning

confidence: 99%

Biochemical assessment of spironolactone oral suspension in human plasma using ultra‐performance liquid chromatography‐tandem mass spectrometry: Application toward pharmacokinetic study

Rajendran,

Chekraverthy,

Peraman

et al. 2024

Separation Science Plus

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Spironolactone is one of the non‐selective mineralocorticoid receptor antagonists acting as anti‐diuretic drugs. Spironolactone is reported to possess mild to severe hyperkalemia which could often result in fatal conditions. Although several analytical methods for evaluating spironolactone as tablet dosage forms have been established in human biological systems, it is crucial to highlight that these approaches are still limited to the suspension dosage form. In this study, we have quantified spironolactone suspension in human plasma with an efficient, sensitive, and optimized ultra‐performance liquid chromatography (UPLC)‐tandem mass spectrometry‐based bioanalytical method using a deuterated internal standard method. A reverse phase UPLC analysis and mass spectrometric detection was performed using electrospray ionization in positive ion mode as an interface, and multiple reaction monitoring as a mode of acquisition. The retention of Spironolactone (SL) and Spironolactone d7 (SL‐d7) was found to be around 6.15 and 6.07 min, respectively. The linearity range was 1.007–100.224 ng/mL for SL with 0.1 mL sample volume. The method was successfully applied to the pharmacokinetic study of spironolactone in healthy male volunteers by administrating 25 mg/5 mL oral suspension and is in accordance with bioanalytical guidelines.

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Section: Methodsmentioning

confidence: 99%