Simultaneous Quantification of Multiple Cancer Biomarkers in Blood Samples through DNA‐Assisted Nanopore Sensing

Liu, Lei; Li, Ting; Zhang, Shouwen; Song, Peng; Guo, Bingyuan; Zhao, Yuliang; Wu, Hai‐Chen

doi:10.1002/anie.201803324

Cited by 78 publications

(57 citation statements)

References 50 publications

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“…[39][40][41][42][43][44][45][46][47][48][49][50][51][52] For example, pores made of proteins (bio-pores) have shown extremely high resolution in analysing targets with small diameters. [53][54][55][56][57][58] The most representative success is the invention of the 4thgeneration of gene sequencing. 40,51,52 For comparison, the other class of pores fabricated using solid-state materials (such as silicon nitride [59][60][61][62][63][64][65] ) offer several potential points of superiority over bio-pores in terms of chemical, mechanical and thermal robustness and better exibility for detecting huge target molecules, which could not pass through bio-pores.…”

Section: Introductionmentioning

confidence: 99%

Exploration of solid-state nanopores in characterizing reaction mixtures generated from a catalytic DNA assembly circuit

Zhu

2019

Chem. Sci.

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We adapt a solid-state nanopore for analyzing DNA assembly mixtures, which is usually a tougher task for either traditional characterization methods or nanopores themselves. A trigger induced nucleic acid amplifier, SP-CHA, is designed as a model. We propose an electrophoresis-gel like, but homogeneous, quantitative method that can comprehensively profile the “base-pair distribution” of SP-CHA concatemer mixtures.

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Section: Introductionmentioning

confidence: 99%

Exploration of solid-state nanopores in characterizing reaction mixtures generated from a catalytic DNA assembly circuit

Zhu

2019

Chem. Sci.

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“…Only a few studies have been published on the detection of biomarkers model using protein nanopores. Most of those studies indirectly detect biomarkers by DNA assisted sensing [126][127][128] . To our knowledge, there are only a few articles on direct detection of biomarkers from serum for normal biological process 107,129 and human disease 34 .…”

Section: Discussion and Perspectivesmentioning

confidence: 99%

Aerolysin, a Powerful Protein Sensor for Fundamental Studies and Development of Upcoming Applications

et al. 2019

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Nanopore electrical approach is a breakthrough in single molecular level detection of particles as small as ions, and complex as biomolecules. This technique can be used for molecule analysis, and characterization as well as for the understanding of confined medium dynamics in chemical or biological reactions. Altogether, the information obtained from these kinds of experiments will allow to address challenges in a variety of biological fields. The sensing, design and manufacture of nanopores is crucial to obtain these objectives. For some time now, aerolysin, a pore forming toxin, and its mutants have shown high potential in real time analytical chemistry, size discrimination of neutral polymers, oligosaccharides, oligonucleotides and peptides at monomeric resolution, sequence identification, chemical modification on DNA, potential biomarkers detection and protein folding analysis. This review focuses, on the results obtained with aerolysin nanopores on the fields of chemistry, biology, physics and biotechnology. We discuss and compare as well the results obtained with other protein channel sensors.For the past two decades, the number of papers and the citations in the field of nanopores has increased exponentially. Nanopore technology has become a sensitive, selective, low cost, label-free, real-time and transportable tool for sensing a wide variety of molecules, including ions, polymers, polyelectrolytes, viruses, ligand-molecule complexes and biomolecules. It allows for the analysis of transport properties, conformations, folding, size, sequence or chemical modifications 1-6 . At first, the protein channels were the main sensors used to perform numerous studies 7-9 . Thanks to material science, chemistry, nanoscience and molecular biology, it is now possible to design and manufacture new classes of nanopores: solid-state sensors 10,11 , DNA origami nanopores 12,13 , carbon 14,15 or cyclodextrin nanotubes [16][17][18] , hybrid nanopores 19-21 and glass 22,23 or quartz nanopores 24 with DNA aptamers 25 . The increased interest in nanopore research has been mainly associated to the ultra-fast DNA sequencing challenge, recently achieved by Oxford Nanopore Technology [26][27][28][29] . The objectives at the horizon of this field comprise of proteomic sequencing 6,30-33 , biomarker detection (of micro-RNAs 34 as well as infinitesimal peptides and proteins quantities) and single-molecule mass spectrometry 32,[35][36][37][38][39][40][41][42] . Up to now, the best sensitivity for biotechnological or heath applications is obtained through biological channels.A member of the pore-forming toxin (PFTs) 43 family, aerolysin is a beta structure toxin that has recently been at the center of extensive fundamental studies and some biotechnology applications 32,38,39,[44][45][46][47][48] . In this review we first introduce the molecular mechanism of channel formation, from soluble inactive monomers to a functional pore into a lipid membrane (figure 1); we delve into the structure of monomeric and heptameric aerolysin along with...

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“…Liu et al fabricated a DNA-assisted nanopore sensor for multiplex biomarker simultaneous detection. In their work, some biomarkers such as PSA, CEA, and AFP linked with different barcodes of DNA to exhibit current blockages [ 175 ].…”

Section: Microstructure-based Biomarker Sensorsmentioning

confidence: 99%

Recent Progress of Biomarker Detection Sensors

Liu

Cui

2020

Research

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Early cancer diagnosis and treatment are crucial research fields of human health. One method that has proven efficient is biomarker detection which can provide real-time and accurate biological information for early diagnosis. This review presents several biomarker sensors based on electrochemistry, surface plasmon resonance (SPR), nanowires, other nanostructures, and, most recently, metamaterials which have also shown their mechanisms and prospects in application in recent years. Compared with previous reviews, electrochemistry-based biomarker sensors have been classified into three strategies according to their optimizing methods in this review. This makes it more convenient for researchers to find a specific fabrication method to improve the performance of their sensors. Besides that, as microfabrication technologies have improved and novel materials are explored, some novel biomarker sensors—such as nanowire-based and metamaterial-based biomarker sensors—have also been investigated and summarized in this review, which can exhibit ultrahigh resolution, sensitivity, and limit of detection (LoD) in a more complex detection environment. The purpose of this review is to understand the present by reviewing the past. Researchers can break through bottlenecks of existing biomarker sensors by reviewing previous works and finally meet the various complex detection needs for the early diagnosis of human cancer.

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Simultaneous Quantification of Multiple Cancer Biomarkers in Blood Samples through DNA‐Assisted Nanopore Sensing

Cited by 78 publications

References 50 publications

Exploration of solid-state nanopores in characterizing reaction mixtures generated from a catalytic DNA assembly circuit

Exploration of solid-state nanopores in characterizing reaction mixtures generated from a catalytic DNA assembly circuit

Aerolysin, a Powerful Protein Sensor for Fundamental Studies and Development of Upcoming Applications

Recent Progress of Biomarker Detection Sensors

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