Simultaneous quantification of major phytohormones and related compounds in crude plant extracts by liquid chromatography–electrospray tandem mass spectrometry

Pan, Xiangqing; Welti, Ruth; Wang, Xuemin

doi:10.1016/j.phytochem.2008.02.008

Cited by 254 publications

(202 citation statements)

References 30 publications

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“…The samples were vortexed and centrifuged at 11,300g for 1 min. The lower phase was collected, and ABA was quantified by MS as described by Pan et al (2008).…”

Section: Aba Contentmentioning

confidence: 99%

Nonspecific Phospholipase C NPC4 Promotes Responses to Abscisic Acid and Tolerance to Hyperosmotic Stress inArabidopsis

et al. 2010

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Diacyglycerol (DAG) is an important class of cellular lipid messengers, but its function in plants remains elusive. Here, we show that knockout of the Arabidopsis thaliana nonspecific phospholipase C (NPC4) results in a decrease in DAG levels and compromises plant response to abscisic acid (ABA) and hyperosmotic stresses. NPC4 hydrolyzes various phospholipids in a calcium-independent manner, producing DAG and a phosphorylated head group. NPC4 knockout (KO) plants display decreased ABA sensitivity in seed germination, root elongation, and stomatal movement and had decreased tolerance to high salinity and water deficiency. Overexpression of NPC4 renders plants more sensitive to ABA and more tolerant to hyperosmotic stress than wild-type plants. Addition of a short-chain DAG or a short-chain phosphatidic acid (PA) restores the ABA response of NPC4-KO to that of the wild type, but the addition of DAG together with a DAG kinase inhibitor does not result in a wild-type phenotype. These data suggest that NPC4-produced DAG is converted to PA and that NPC4 and its derived lipids positively modulate ABA response and promote plant tolerance to drought and salt stresses.

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“…The samples were vortexed and centrifuged at 11,300g for 1 min. The lower phase was collected, and ABA was quantified by MS as described by Pan et al (2008).…”

Section: Aba Contentmentioning

confidence: 99%

Nonspecific Phospholipase C NPC4 Promotes Responses to Abscisic Acid and Tolerance to Hyperosmotic Stress inArabidopsis

et al. 2010

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“…For this purpose, we adopted a recently described protocol to measure phytohormones (Pan et al, 2008) but used ultra performance liquid chromatography Fourier transformion cyclotron resonance mass spectrometry for the determinations. By comparison with spectra obtained from authentic chemical standards, we were able to quantify the relative levels of ABA, JA, and the GAs GA1 and GA3 (Fig.…”

Section: Evaluation Of Hormone Metabolismmentioning

confidence: 99%

“…Extraction of hormones from young fruit was carried out as described by Pan et al (2008), with the exception that 10 g of tissue and a lengthened inhibition period were used, as described by van der Merwe et al (2009). In brief, the liquid nitrogen-frozen pericarp material was ground into a fine powder.…”

Section: Hormone Analysismentioning

confidence: 99%

RNA Interference of LIN5 in Tomato Confirms Its Role in Controlling Brix Content, Uncovers the Influence of Sugars on the Levels of Fruit Hormones, and Demonstrates the Importance of Sucrose Cleavage for Normal Fruit Development and Fertility

et al. 2009

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It has been previously demonstrated, utilizing intraspecific introgression lines, that Lycopersicum Invertase5 (LIN5), which encodes a cell wall invertase, controls total soluble solids content in tomato (Solanum lycopersicum). The physiological role of this protein, however, has not yet been directly studied, since evaluation of data obtained from the introgression lines is complicated by the fact that they additionally harbor many other wild species alleles. To allow a more precise comparison, we generated transgenic tomato in which we silenced the expression of LIN5 using the RNA interference approach. The transformants were characterized by an altered flower and fruit morphology, displaying increased numbers of petals and sepals per flower, an increased rate of fruit abortion, and a reduction in fruit size. Evaluation of the mature fruit revealed that the transformants were characterized by a reduction of seed number per plant. Furthermore, detailed physiological analysis revealed that the transformants displayed aberrant pollen morphology and a reduction in the rate of pollen tube elongation. Metabolite profiling of ovaries and green and red fruit revealed that metabolic changes in the transformants were largely confined to sugar metabolism, whereas transcript and hormone profiling revealed broad changes both in the hormones themselves and in transcripts encoding their biosynthetic enzymes and response elements. These results are discussed in the context of current understanding of the role of sugar during the development of tomato fruit, with particular focus given to its impact on hormone levels and organ morphology.The importance of the supply to, and the subsequent mobilization of Suc in, plant heterotrophic organs has been the subject of intensive research effort over many

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“…Both wound and pathogen Botrytis cinerea treatment induce biosynthesis of ABA, JA, and SA (Pan et al, 2008) suggesting synergistic or antagonistic interactions among these induced hormone signaling to generate defense responses. MeJA (methyl jasmonate) treatment induces stomatal closing through a CORONATINE INSENSITIVE1 (COI1)-and JASMO-NATE RESISTANT1 (JAR1)-dependent signaling pathway (Munemasa et al, 2007;Suhita et al, 2004).…”

Section: Interaction Of Aba Signaling With Biotic Stress Response Patmentioning

confidence: 99%

Plant Stress Surveillance Monitored by ABA and Disease Signaling Interactions

Kim

2012

Molecules and Cells

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Simultaneous quantification of major phytohormones and related compounds in crude plant extracts by liquid chromatography–electrospray tandem mass spectrometry

Cited by 254 publications

References 30 publications

Nonspecific Phospholipase C NPC4 Promotes Responses to Abscisic Acid and Tolerance to Hyperosmotic Stress inArabidopsis

Nonspecific Phospholipase C NPC4 Promotes Responses to Abscisic Acid and Tolerance to Hyperosmotic Stress inArabidopsis

RNA Interference of LIN5 in Tomato Confirms Its Role in Controlling Brix Content, Uncovers the Influence of Sugars on the Levels of Fruit Hormones, and Demonstrates the Importance of Sucrose Cleavage for Normal Fruit Development and Fertility

Plant Stress Surveillance Monitored by ABA and Disease Signaling Interactions

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