Simultaneous quantification of intracellular concentrations of clinically important metabolites of folate-homocysteine cycle by LC-MS/MS

Golja, Maša Vidmar; Trontelj, Jurij; Geršak, Ksenija; Mlinarič-Raščan, Irena; Šmid, Alenka

doi:10.1016/j.ab.2020.113830

Cited by 8 publications

(3 citation statements)

References 36 publications

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“…By XRD characterization of solid BL-CQDs, the scanning range was set at 10°-60°, as shown in figure 2(c), and a wide diffraction peak was displayed at 2θ = 20.16°, indicating that the carbon atoms of BL-CQDs showed a highly disordered state. Compared with standard cards, it was found that the peak corresponded to the (002) plane of graphene [17] which was consistent with the HR-TEM results of BL-CQDs, confirming the graphene-like carbon structure of BL-CQDs.…”

Section: Bl-cqds Characterizationsupporting

confidence: 82%

Ratiometric fluorescence detection of the angiotensin-converting enzyme via single-excitation and double-emission biomass-derived carbon quantum dots

Zhan,

Mao,

Xue

et al. 2023

Methods Appl. Fluoresc.

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Efficient and rapid detection of angiotensin-converting enzyme (ACE) activity is important for preventing hypertension and the discovery of new angiotensin-converting enzyme inhibitors (ACEI). In this work, a single-excitation and double-emission biomass-derived carbon quantum dots (CQDs) was prepared and applied for ratiometric fluorescence detection of ACE. Fresh banyan leaves were extracted with ethanol and acetone, and the extracted solution was used as the precursor to produce the carbon quantum dots (BL-CQDs) with single-excitation and double-emission properties. The synthesized BL-CQDs is about 1.7 nm, has a graphene-like structure, contains a variety of hydrophilic functional groups on the surface, and has good fluorescence properties. Its fluorescence intensity ratio (I677/I460) is linear with ACE activity in the range of 0.02-0.8 U/L. The regression equation is △F=2.5371 CACE-0.0311. The method was successfully applied to the determination of ACE activity in pig lung and human serum, and the inhibitory efficiency of the flavonoid extract and captopril tablets on ACE activity was also investigated, which can be applied to the screening of ACEI. The survival rate and fluorescence imaging of Bel-7404 cells under the condition of high concentration BL-CQDs showed BL-CQDs had low cytotoxicity and good biocompatibility. These results indicate that the BL-CQDs can be used as an excellent fluorescent probe, providing a new method for screening ACE activity and plant-derived ACEI.

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Section: Bl-cqds Characterizationsupporting

confidence: 82%

Ratiometric fluorescence detection of the angiotensin-converting enzyme via single-excitation and double-emission biomass-derived carbon quantum dots

Zhan,

Mao,

Xue

et al. 2023

Methods Appl. Fluoresc.

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“…However, folate deficiency is widespread and occurs worldwide, both in developing and developed countries [ 3 ]. It is considered as one of the most common nutritional deficiencies which can lead to a variety of disorders including neural tube defects, megaloblastic or macrocytic anemia, occlusive vascular disease, and others [ 4 , 5 ]. On the other hand, excessive folate intake has also been associated with adverse health effects, such as autism, negative neurocognitive development, and some cancers [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…In folate metabolism, there are five one-carbon substituted folate derivatives in vivo, and these derivatives carry one-carbon units ranging from formate to methanol and serve unique one-carbon transfer reactions. The predominant folate in blood is 5-CH 3 THF, and it delivers its own methyl groups to Hcy to form Met and THF [ 5 , 17 ]. Furthermore, some of the Met is then transformed to form SAM under catalysis by Met adenosyltransferase.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of One-Carbon Folate Metabolites and One-Carbon-Related Amino Acids in Biological Samples Using a UHPLC–MS/MS Method

Ling,

Tan,

Wang

et al. 2024

IJMS

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One-carbon folate metabolites and one-carbon-related amino acids play an important role in human physiology, and their detection in biological samples is essential. However, poor stability as well as low concentrations and occurrence in different species in various biological samples make their quantification very challenging. The aim of this study was to develop a simple, fast, and sensitive ultra-high-performance liquid chromatography MS/MS (UHPLC–MS/MS) method for the simultaneous quantification of various one-carbon folate metabolites (folic acid (FA), tetrahydrofolic acid (THF), p-aminobenzoyl-L-glutamic acid (pABG), 5-formyltetrahydrofolic acid (5-CHOTHF), 5-methyltetrahydrofolic acid (5-CH3THF), 10-formylfolic acid (10-CHOFA), 5,10-methenyl-5,6,7,8-tetrahydrofolic acid (5,10-CH+-THF), and 4-α-hydroxy-5-methyltetrahydrofolate (hmTHF)) and one-carbon-related amino acids (homocysteine (Hcy), methionine (Met), S-ade-L-homocysteine (SAH), and S-ade-L-methionine (SAM)). The method was standardized and validated by determining the selectivity, carryover, limits of detection, limits of quantitation, linearity, precision, accuracy, recovery, and matrix effects. The extraction methods were optimized with respect to several factors: protease–amylase treatment on embryos, deconjugation time, methanol precipitation, and proteins’ isoelectric point precipitation on the folate recovery. Ten one-carbon folate metabolites and four one-carbon-related amino acids were detected using the UHPLC–MS/MS technique in various biological samples. The measured values of folate in human plasma, serum, and whole blood (WB) lay within the concentration range for normal donors. The contents of each analyte in mouse plasma were as follows: pABG (864.0 nmol/L), 5-CH3THF (202.2 nmol/L), hmTHF (122.2 nmol/L), Met (8.63 μmol/L), and SAH (0.06 μmol/L). The concentration of each analyte in mouse embryos were as follows: SAM (1.09 μg/g), SAH (0.13 μg/g), Met (16.5 μg/g), 5,10-CH+THF (74.3 ng/g), pABG (20.6 ng/g), and 5-CH3THF (185.4 ng/g). A simple and rapid sample preparation and UHPLC–MS/MS method was developed and validated for the simultaneous determination of the one-carbon-related folate metabolites and one-carbon-related amino acids in different biological samples.

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Preparation of thiol-free amino acid plasma matrix for quantification of thiol amino acids and their oxidized forms in human plasma by UPLC-MS/MS

Tao,

Cao,

Zhang

et al. 2024

Journal of Chromatography A

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Simultaneous quantification of intracellular concentrations of clinically important metabolites of folate-homocysteine cycle by LC-MS/MS

Cited by 8 publications

References 36 publications

Ratiometric fluorescence detection of the angiotensin-converting enzyme via single-excitation and double-emission biomass-derived carbon quantum dots

Ratiometric fluorescence detection of the angiotensin-converting enzyme via single-excitation and double-emission biomass-derived carbon quantum dots

Simultaneous Determination of One-Carbon Folate Metabolites and One-Carbon-Related Amino Acids in Biological Samples Using a UHPLC–MS/MS Method

Preparation of thiol-free amino acid plasma matrix for quantification of thiol amino acids and their oxidized forms in human plasma by UPLC-MS/MS

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